ISAzvi7

  • Family IS4
  • Group IS50
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP001157 ND Azotobacter vinelandii
Azotobacter vinelandii AvOP
DNA section
IS Length : 1450 bp

Ends


IR Length : 16/17

IRL : CTCGTCGTTATACACAACTCCAGTCCCCACAGGTCTATACCCAAAAGGGA
IRR : CTCGTCGTTATACATAAGTTCCGGCGAGCGCATCGCGTGCTTCCAGTGCA

Insertion site


Left flankDirect repeatRight flankDR Length
CCCCCTCGCGCGCGAGGGCG0

DNA sequence

CTCGTCGTTATACACAACTCCAGTCCCCACAGGTCTATACCCAAAAGGGATTCGTGGAGAATGACGATGAACTGGGCAGAGGAAGAAATGCAGACCGCCG
ACCTGGGCGATGAGCGCCTGAATGTACGGGTAGCCAAGGTCCTGGAGCGGTTGGGAGCGCACCCGGGGAGCAGCATTCCCGCGGCCTGCCGGGGCTGGGC
GGAAACGATGGCGGCCTATCGGTTCTTCGACCACGAGAAGGCAACATTCGAGACGGTGCTGACACCGCATCGGGATGCCACGCTGCAGCGCATGGAGTGT
TGCCCGGTGGTGTTGCTGGTACAGGACACCACCGAGCTCGACAAGTGGGTGAGCCTGGGCCCCAAGGGCGTGGGCACGCTCAAGGAGCAGCAGAAATACC
CGCGTCGGTTGCATCCGACCGTGGCCTTTACCCCAGAACGCATCTGCCTTGGGGTCGTGGAGGCCTTGTGGTGGAGCCGCGACGAACCGAGTCCGCGCAA
GGCGCGTCGTGGCAAGGGTGTGGACGAGAAGGAGAGCCGGCGCTGGATCGACAGTTACCAGGCTAGCTGCGCGTTGCAGGGGCAGATCCCCAAAACGCAA
TTGGTGAACCTCGCCGATGCGGAAGGGGATCTGTATGAATGGTTCACCGAGTATGCGGAAGTCGCACCGTCCACGCGGGCGCAGTGGATCGTGCGTGCGG
CACAGGATCGACGCGTGCTGACGGGCGATGCCGACAAGCTATGGGCATCGCTGGCCATGGCGCCGGGTCTGGGGCAGCTCGCCGTCGAGGTCCGGGCACG
CCCGAAGCGCCCGGCCCGGCAGGCCCGGGTCACGTTGCGTTCGGCCACTGTCGTGCTGCGTCCCCCCGCTCGAATCGGTCGCCACTTGCCGGAAGTCTCC
GTCAATGCCGTGCTGGCCCGCGAAGAGAATCCTCCGGAGGGCGTCGAGCCGCTGGAGTGGCTGTTGCTGACCAGCTTGCCGGTGGGCAGCCTGGAACAGG
CCTCTACGATTGTCGCCTGGTATGCCGTGCGCTGGTATATCGAGATTTACTTTCATGTCCTGAAAAACGGCTGTCAGATCAATTGCCTGCAACTGGAAAC
CGAGGAGCGGCTACTGCCTTGCATCGGGCTTTACCTGGTGGTTGCCTGGCGAGTGCTGTACAGCCTGATGCTCGGACGTGCCTGCCCGGAGCTGAATTGC
GAGTTGATTTTCGAAGCCCGGGAGTGGCGGGCCGCCTACCTCGTGATCAAGCGCTGCCCACCGCCGCCGGTGCCACCTCGACTGGGCGAGATGGTTGAGT
GGGTGGCCGGTCTGGGCGGCTATCTGGGGCGCAAACACGATGGCCCACCCGGCCCCAAGGCCATGTGGATCGGATTGCAGCGATTGCGGGAGTTTGTCAT
TGCACTGGAAGCACGCGATGCGCTCGCCGGAACTTATGTATAACGACGAG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1380 bp460 aa611440+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTMNWAEEEMQTADLGDERLNVRVAKVLERLGAHPGSSIPAACRGWAETMAAYRFFDHEKATFETVLTPHRDATLQRMECCPVVLLVQDTTELDKWVSLG
PKGVGTLKEQQKYPRRLHPTVAFTPERICLGVVEALWWSRDEPSPRKARRGKGVDEKESRRWIDSYQASCALQGQIPKTQLVNLADAEGDLYEWFTEYAE
VAPSTRAQWIVRAAQDRRVLTGDADKLWASLAMAPGLGQLAVEVRARPKRPARQARVTLRSATVVLRPPARIGRHLPEVSVNAVLAREENPPEGVEPLEW
LLLTSLPVGSLEQASTIVAWYAVRWYIEIYFHVLKNGCQINCLQLETEERLLPCIGLYLVVAWRVLYSLMLGRACPELNCELIFEAREWRAAYLVIKRCP
PPPVPPRLGEMVEWVAGLGGYLGRKHDGPPGPKAMWIGLQRLREFVIALEARDALAGTYV

 

Blast result :
Comments
ISAzvi7 is 56% aa similar to ISAzo5. ISAzvi7 was found by screening completely sequenced genomes for sequences homologous to the IS50R transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-96-D(N3)-141-E(C1). Genome sequencing of Azotobacter vinelandii AvOP is currently in progress. Accession number: NZ_AAAU00000000.
References
1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,C., Glavina,T., Hammon,N., Israni,S., Pitluck,S. and Richardson,P. (2006) Unpublished