ISfinder Mirror Site

ISAzo9

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_006513	ND	Azoarcus sp.	Azoarcus sp. EbN1

DNA section

IS Length : 1483 bp

Ends

IR Length : 26/30

IRL : GTACCTCTGCACAGAGGATTTGACGGGTTGATGGTCGGGTGAGCGCAGGA
IRR : GTACCTCTGCACAGAGGTTATGACTGTTTGGCTGGGTGCGGGTAAGCGCG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GTGAAGAAGC	TA	GCGGTATCAC	2

DNA sequence

GTACCTCTGCACAGAGGATTTGACGGGTTGATGGTCGGGTGAGCGCAGGAGGGGCAGGGCTTCGGGCGGGGCGAGGAGTCGGATGCTGCGCGCGACTCCA
GGTTGTCGGCTGAGGAGTCCCAGGCGCTCGAGCGTGAGCACCATCTGATGCACCGAGGGGGGCGTGACGTGGAAGTAGCGTTGCAGGTCGGCTTCGGCCG
GCGGCCGGCCGAGCACTTGGGTGTAAGCGTAGATGAACGCGAGGTACTGCCCTTGCACCGGTGTGAAGCGCGGTGACGAAGGGGTGAGTTCCGCGTCGGA
GTTCTGGCTCATTTGTTGTCTCTCCAGATTTTGGAGGGGCCGCGATGAATGTACGCTACCGGGTCGAATTGAGCCAAGCCGAACGCGACGAATTGAGGAT
GCTGCTGGGCGGCGGCAAGGCAGCGGTGCGCAAGGTGAAACGCGCGCAGATCCTGCTGGCGGCCGATGCCGGAGCCGGCGACGAGGAGATTGCCACGAAC
GTCGGAGTGGGCAGCTCGACGGTCTATCGGACCAAGCGCCGCTTCGTGCTGGGCAACGTCGAGGCGGCGCTCAGCGACGAGCCGCGCCCCGGGGCGGAGC
GCAAGCTCACCGGCAAGGAAGAGGCGCTGCTGGTCGCCACCGCCTGCACCAAGCCGCCAACGGGGCGGTCGCGCTGGACGCTCGAATTGCTGGCGGGGGA
GATAGTCAAGCTCACCGAGCACGCGAGCCTGTCACGCGAGACGGTGCGCCGCCGCCTGGCGGAGAACGAGCTCAAGCCTTGGCGCCAGAAGATGTGGTGC
ATCCCGCAGGTCGACGGTCACTACGTTGCCCGGATGGAGGACGTGCTCGACCTCTACGCCGAAGCCCCCGATCCCGAGCGCCCGGTGGTCTGCTTCGATG
AAAGTCCCACCCAGCTCATCGGCGAGGTGCGCCAGCCCATCCCGGCGATGCCCGGACAGCTCGAGCGCTACGACTACGAGTATCGCCGCAACGGGACCGC
CAACCTGTTCGTCTTCCTCGACGCGCACCGGTCCTGGCGCCGGGTCAAGGTAACCGAGCAGCGCACTGCGCTCGACTTCGCCGAATGCATGCGCGAACTG
GTCGACTGCCACTATCCGCAGGCCGAGCGTATCCGTGTTGTCCTCGACAACCTGTCGACCCATACGCCCGGCGCACTCTATGAAACCTTCCCGGCGCCCG
AAGCGCATCGGTTGCTGCATCGTCTGGAATTCCATTACACCCCCAAGCACGCCAGCTGGCTCAACATGGTCGAGATCGAAATCGGGGTGCTGCGCAGCCA
GTGCCTCGACCGGCGCATCGGCGACCGCGACCGACTCGTCAGCGAAATTGCAGCCTGGGAGCAGCAGCGCAACGCCATCGGCGCTCGCATCCACTGGATG
TTCACCACCGACCGCGCCCGGAGCAAACTGGCCCGCGCTTACCCGCACCCAGCCAAACAGTCATAACCTCTGTGCAGAGGTAC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
312 bp	104 aa	1	312	-	No

ORF function : Passenger Gene
Annotation : Helix-turn-helix diphteria tox regulatory ElementDescription :

ORF sequence :

MSQNSDAELTPSSPRFTPVQGQYLAFIYAYTQVLGRPPAEADLQRYFHVTPPSVHQMVLTLERLGLLSRQPGVARSIRLLAPPEALPLLRSPDHQPVKSS
VQRY

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1122 bp	373 aa	345	1466	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNVRYRVELSQAERDELRMLLGGGKAAVRKVKRAQILLAADAGAGDEEIATNVGVGSSTVYRTKRRFVLGNVEAALSDEPRPGAERKLTGKEEALLVATA
CTKPPTGRSRWTLELLAGEIVKLTEHASLSRETVRRRLAENELKPWRQKMWCIPQVDGHYVARMEDVLDLYAEAPDPERPVVCFDESPTQLIGEVRQPIP
AMPGQLERYDYEYRRNGTANLFVFLDAHRSWRRVKVTEQRTALDFAECMRELVDCHYPQAERIRVVLDNLSTHTPGALYETFPAPEAHRLLHRLEFHYTP
KHASWLNMVEIEIGVLRSQCLDRRIGDRDRLVSEIAAWEQQRNAIGARIHWMFTTDRARSKLARAYPHPAKQS

Blast result :

Comments

The first ORF is a passenger gene, the second is the transposase. ISAzo9 is 63% aa similar to ISMac17.

References

1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.

2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.

3] PROSCIENCE (2004) Direct Submission GenBank.

4] ISfinder annotation (2008).