ISfinder Mirror Site

ISAzca2

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_009937	ND	Azorhizobium caulinodans	Azorhizobium caulinodans ORS 571

DNA section

IS Length : 2458 bp

Ends

IR Length : 19/25

IRL : GTAAGCGTCGTCCTACCCCCACCTTTCGCCGGCGAGGCTGAGGGGGCGAT
IRR : GTAAGCGTCGTCTTCAGGCCACGTTGCTGAGGACCGGCTGTGCTGGGTAG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GTCACAAACG	GC	TTTTAGGGGG	2

DNA sequence

GTAAGCGTCGTCCTACCCCCACCTTTCGCCGGCGAGGCTGAGGGGGCGATGTTCCGAGCCTGGAGGGCTTGGACATATGACGATTTCAGAGCTTACGCTT
AAATCTAGCGCCGAGGAGCCGGTGCGGCGGTTCGAGGTTTTCACCGGAGCCGGGCGTCGCCGAGAGTGGGCTCCCGAGGAGAAGGCGCGGATTGTCACCG
AGAGCTTTGAGCCTGGAGCCACGGTGAGTGCAGTGGCGCGCCGCCACGGACTGTCGCCGCAGCAGTTGTTCACGTGGCGCCGTGAGGCGCGGAAAGCGAG
CGAGACGATCCCGGCTTTCGTGCCGGCGGTGGTCGCTCCGGAACCGGTTCCCGCATCTGAGCCGTCCGCCCAACCGTCACGGCCGAAGCCGCGTGATCGG
CAACGTCGCGCGGCAGCCATCGAGGTCGACGTCGCCGGGGTCAGGGTGACGATTGAGAACGGGGCCTCGCCCGCCACCATCGCAGCGGTGCTCGGCGCGC
TGAAGGCCGGATCGTGATCGGCCCGAGCGGCGCGGTCCGGGTCATGGTGGCGACACGGCCGGTGGACTTCCGCAAGGGGGCGGAGGGTCTCGCCGCTTTG
GTGCGCGAGACCATGGGCGCCGATCCGTTCTCCGGCACCGTGTACGTGTTCCGGGCCAAGCGAGCGGACCGGGTGAAGCTGGTGTTCTGGGACGGCACCG
GCGTCGTGCTGGTGGCGAAGCGGCTTGAGGACGGGGAGTTCCGCTGGCCGAAGATCGAGGACGGCACCCTGCAGCTGACGTCGGCCCAGCTCCAGGCCCT
GCTCGAGGGCCTCGACTGGCGGCGAGTGCATGAGGCGCAGCGCACGATGACACCGGTCACCGCCAGCTGAAGAGGCTCGCAGGGGCCTGGCGAATGTGAT
TCACTTCCGGTCATGGCCTCGCTCTCCACCGAGCCCCCCCGTGACCCCGAGACGCTCCAGGCGATGCTGATCGCGCGGGACACGGAGATCGAGCGCCTGC
GCCAGATCATCAAGGAGTTGCAGCGCCACCGCTTCGGACGGCGGGCGGAGAGCCTGCCCGAGGACCAACTGCTGCTGGCGCTCGAAGAGGCCGAACAGGT
CGAGGCTGCCTACTTCGCCGCGTCCGATGAACAGGTGCCGGCCGAGAAGGCCGAGCGGGTGGCCAAACGCCGTGCGAACCGCGGTGCTTTGCCCGCCCAT
CTGCCGCGGATCGAGACCGTGATCGATATCGAGAGTGACATCTGCCCCTGCTGTTCCGGCAAGCTGCACCGCATCGGCGAGGATGTGGCGGAACGGCTCG
ACATGGTGCCTGCCCAGTTCCGCGTCCTGGTGGTGCGGCGGCCGAAATATGCCTGCCGGTCCTGCGCGGACGTGGTGGTGCAGGCCCCCGCTTCGGCTCG
GTTGATCGAAGGTGGCATCCCGACCGAGGCAACCGTCGCCCATGTGCTGGTCTCGAAATACGCCGACCATCTGCCCCTGTATCGCCAAGTCCAGATCTAT
GCGCGGCAGGGCATCGCGCTCGACCGCTCGACGCTGGCGGACTGGGTCGGGCGCGCCGCCTTCCTGCTGCGACCTGTCCACGAGCGGCTGCTGGCGATGC
TAAGGGCCTCGACCAAGCTGTTCGCCGACGAGACCACGGCGCCGGTGCTCGATCCCGGCCGTGGCCGCACCAAGACCGGGCAGCTCTTCGCCTATGCCCG
TGATGACCGTCCGTGGGGCGGAGCCGATCCGCCCGCCGTCGCTTATGTCTATGCCCCCGATCGTAAGAGCGAGCGGCCGGTCGCCCACCTCGACGGCTTC
AGCGGCATCCTGCAGGTCGATGGCTATGGCGGCTACAAGGCGCTAGCCGGACGCAACGCCGTGAATCTGGCGTTCTGCTGGGCCCACGTGCGCCGCCGCT
TCTACGAGCTCGCTCAGAGCGGTCCCGCGCCGATCGCCAGCGAGGCCCTGCAGCGCATCGCCGAGCTCTATCGCCTCGAGGCTGCGATCCACGGCCGAAC
CCCAGATGAGCGCCGAGCCTCGCGCCAAGAGAAGAGCAGGCCGATCATCGAGGCCATGGAGCCATGGCTGCGCGAGAAGCTGGCTCTGATCAGCCAGAAG
ACCAAGCTGGCCGAGGCGATCCGCTATGCGCTCTCGCGCTGGCAGGGGCTGACCCCGTTCCTCGACGACGGCCGCGTCGAGTTGGACAACAACATCGTCG
AGCGCTCCATCCGACCCCTGGCCCTGACCCGGAAGAATGCGCTCTTCGCCGGCTCCGACGGCGGCGCCGAGCACTGGGCTGTCATGGCTTCGCGGGTCGA
GACCTGCAAGCTCAACGACATCGACCCCCAGGCCTACCTCGCGGACGTCATCACCCGCATCGTCAACAGCCACCCCAACAGCCGCATCGACGACCTCATG
CCATGGGCCTACCCAGCACAGCCGGTCCTCAGCAACGTGGCCTGAAGACGACGCTTAC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
441 bp	146 aa	77	517	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MTISELTLKSSAEEPVRRFEVFTGAGRRREWAPEEKARIVTESFEPGATVSAVARRHGLSPQQLFTWRREARKASETIPAFVPAVVAPEPVPASEPSAQP
SRPKPRDRQRRAAAIEVDVAGVRVTIENGASPATIAAVLGALKAGS

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
357 bp	118 aa	514	870	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

VIGPSGAVRVMVATRPVDFRKGAEGLAALVRETMGADPFSGTVYVFRAKRADRVKLVFWDGTGVVLVAKRLEDGEFRWPKIEDGTLQLTSAQLQALLEGL
DWRRVHEAQRTMTPVTAS

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1533 bp	510 aa	913	2445	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MASLSTEPPRDPETLQAMLIARDTEIERLRQIIKELQRHRFGRRAESLPEDQLLLALEEAEQVEAAYFAASDEQVPAEKAERVAKRRANRGALPAHLPRI
ETVIDIESDICPCCSGKLHRIGEDVAERLDMVPAQFRVLVVRRPKYACRSCADVVVQAPASARLIEGGIPTEATVAHVLVSKYADHLPLYRQVQIYARQG
IALDRSTLADWVGRAAFLLRPVHERLLAMLRASTKLFADETTAPVLDPGRGRTKTGQLFAYARDDRPWGGADPPAVAYVYAPDRKSERPVAHLDGFSGIL
QVDGYGGYKALAGRNAVNLAFCWAHVRRRFYELAQSGPAPIASEALQRIAELYRLEAAIHGRTPDERRASRQEKSRPIIEAMEPWLREKLALISQKTKLA
EAIRYALSRWQGLTPFLDDGRVELDNNIVERSIRPLALTRKNALFAGSDGGAEHWAVMASRVETCKLNDIDPQAYLADVITRIVNSHPNSRIDDLMPWAY
PAQPVLSNVA

Blast result :

Comments

orfA, orfB and orfC of ISAzca2 are respectively 62%, 87% and 82% aa similar to orfA, orfB and orfC of ISFpe1.

References

1] Suzuki,S., Aono,T., Lee,K.B., Suzuki,T., Liu,C.T., Miwa,H., Wakao,S., Iki,T. and Oyaizu,H. (2007) Appl. Environ. Microbiol. 73 (20), 6650-6659.
2] Lee,K.B., Aono,T., Kaneko,T. and Oyaizu,H. (2007) Direct Submission GenBank.
3] ISfinder annotation (2008)