ISfinder Mirror Site

ISAzo32

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_006824	ND	Azoarcus sp.	Aromatoleum aromaticum EbN1 Azoarcus sp. EbN1 Azoarcus sp. EbN1 plasmid 2

DNA section

IS Length : 1100 bp

Ends

IR Length : 18/24

IRL : CTAACATTCTCGTAAGTATTGTCACACCATGAGACAATACGCGGATGAAA
IRR : CTAACTTTTTAAAAAGTAATGTCAGATAAGTGCGTATTGGCACTCGGGCT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ACGAAAGTGA	TA	ATTCGTCCCG	2
ATTTGTCGCT	TA	CCACCGGCAA	2

DNA sequence

CTAACATTCTCGTAAGTATTGTCACACCATGAGACAATACGCGGATGAAATGCAAACGGAACTCGGACGGTCGTGCGATCGACCACCACTCGCTTCAGGT
GATGCGCCAGCAGGCGATCAAGGCGGTTCGGGAAGGCCAGACGGTGCAAAGCGTAGCGGCCGCCTTCGGCGTGAATGTGCGCAGCGTCTTTCGGTGGCTG
GCCGACTTCGCCCAGGGCGGGCAAAACGCGTTGCTCGCCAAGCCGATTCCCGGGCGCCCGTCGAAAGTGAGCACGGAGGAGATGCGCTGGCTCGCGCAGG
CGGTGCGGGACCACACGCCGCTGCAGTACAAGTTCGAGTTCGGGCTGTGGACGCTGTCGCTGATCCGCGCGCTGATTCATCGGCAGTTCGGCAAGGCGCT
GTCGATCGCCACCGTCAGCCGGCTCATGAAAGGACTCGGCTTCAGCGCGCAGAAGCCGCTCTACCAGGCATGGCAACAGGACGCCGCGCTGGTGCGGCAA
TGGGAGGCGGAGACCTATCCGGCGATCCGCTCCGAAGCGCGTGCGGCGGGCGCGACGATCTACTTCGCCGACGAGTCGGGCATCCGTTCCGACTACCACA
CCGGCACGACGTGGGCGCCGCGTGGGCAGACGCCGGTGGTCGAAGTGACCGGCCGGCGCTTCTCGCTGAACATGATCTCGGCGGTGAGCCCGCAGGGCGA
ATTTCGCTTCATGCTGCACGAAGGCTCGGTCACCGCCACGGTGTTTCGCGAGTTCCTCAAGCGGTTGATGATCGGCGCCGACAAACCGGTCTTCGTCATC
GTCGACGGGCACCCCATTCACAAGGCCAAGTTGGTTCGGGCCTACGTCGACAGCCTCGAAGGCCAACTCAGGCTCTTCTACCTGCCGCCGTACTCGCCGC
AACTGAACCCCGACGAACAGGTCTGGGCGCACGTGAAGCGTCAGGTCTCGAAGCGCCTGGTGCAGGACAAGGACGAAATGAAGCGCCTGGCCTTGGGCGC
GCTGCGCCGGATTCAACGACTGCCCAGCCTCGTCAAATCGTTCTTCCTTCAGCCCGAGTGCCAATACGCACTTATCTGACATTACTTTTTAAAAAGTTAG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1035 bp	344 aa	45	1079	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKCKRNSDGRAIDHHSLQVMRQQAIKAVREGQTVQSVAAAFGVNVRSVFRWLADFAQGGQNALLAKPIPGRPSKVSTEEMRWLAQAVRDHTPLQYKFEFG
LWTLSLIRALIIRQFGKALSIATVSRLMKGLGFSAQKPLYQAWQQDAALVRQWEAETYPAIRSEARATGATIYFADESGIRSDYHTGTTWAPRGQTPVVE
VTGRRFSLNMISAVSPQGEFRFMLHEGSVTATVFREFLKRLMIGADKPVFVIVDGHPIHKAKLVRAYVDSLEGQLRLFYLPPYSPQLNPDEQVWAHVKRQ
VSKRLVQDKDEMKRLALGALRRIQRLPSLVKSFFLQPECQYALI

Blast result :

Comments

ISAzo32 is 93% aa similar to IS885.

References

1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.

2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.

3] PROSCIENCE (2004) Direct Submission GenBank.

4] ISfinder annotation (2008).