ISAzo34

  • Family IS256
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_006824 ND Azoarcus sp.
Aromatoleum aromaticum EbN1
Azoarcus sp. EbN1 plasmid 2
DNA section
IS Length : 1439 bp

Ends


IR Length : 25/34

IRL : GAGATTTAGTCAAGACTGGTGTATGACCAGATCCTGATAGAAGGGCTTGA
IRR : GAGCTAAAGTCAATTCTGGTGTATGGACCCATCCGGCATGGCTGATCAGG

Insertion site


Left flankDirect repeatRight flankDR Length
GGTCAGGGAGACGACCCCGCCCTTGGATG9

DNA sequence

GAGATTTAGTCAAGACTGGTGTATGACCAGATCCTGATAGAAGGGCTTGATGAGGCGGCGTGCCTCTGCTGAGAATTGAGTTGTCGAGACCAAATTTTCA
GCAACCACGGAGGCGCGCCTTGAAGAAGGATACGAGTGAGCAGTCGGTTGAGCAATCGGAAGTGGGGCTGTCCCTGGAGGAGCTGATCCGGCGCGGCGCG
CGGGATCTCATCGAGCGGGCGATCGAGGTGGAGGTGCAGCAGTTGTTGGCCGAGTACGAGAACGTGCGGATGCTGGGCGGGAGCCGAGCGGTGGTCAGAA
ATGGCTACCTGCCGGCACGCGAGGTGCTCACGGCGGTCGGCAACGTCGAGGTGCGGGTACCCAAGGTGCGTGACCGCTCGGGCGCTGGGGTGAAGTTCAA
TTCCGCCTTGGTGCCGCCGTATGTTCGCCGCTCGGCGCGGGTGTCGGCGGCGTTGCCGTGGCTGTACCTGAAGGGCATCTCGACGGGCGACATGCGCGAG
GCGCTCACCGTGCTGCTCGGCGATGAGGCCAAGGGCCTGTCCCCCAACGTGGTGAGCCGCCTGAAGACCGAGTGGGCGAGCGACTACGCCGGCTGGATGA
AGCGGGATTTGTCCGGCAGCCGCTACGTCTATTGGTGGGCCGACGGCGTGCACACCAGCCTGCGCGGCGAGGACGATGCCCGCCAGTGTCTGCTGGTCGT
CATCGGCGTGCGGCTCGACGGCACCAAGGAACTGGTCACGGTCGGCGACGGGCACCGCGAATCGAAGGCGTCCTGGCTCGAGTTGCTGCGCGACCTCAAG
GCTCGTGGCCTCGAAGTCGGTCCGCGCCTGGCAGTAGGCGACGGGGCGCTGGGCTTCTGGGGCGCGCTCGACGAGCTCTACCCCGAAACCCGCCGGCAGC
GCTGCTGGGTGCACAAGAGCGCCAACGTGCTCAACGCGCTCAACGCGCTGCCCAAGAGCCTGCAGGCCAAGGCGAAGGCGCAGCTCCACGAGATTTGGAT
GGCCCCGACGCGCGCGGCCGCCGTGAGCGCCTTCGAGCGCTTCGTCGAGTGCTACCAGGCGAAGTACCCGAAGGCGGCCGACAAGCTCGTCAGGGATCGC
GACGCGCTGCTCGCCTTCTACGACTTCCCGGCCGAGCACTGGATTCACCTGCGCACGACCAACCCGATCGAGTCGACCTTCGCCACCGTGCGTCACCGCA
CCACCCGCACGAAGAACTGCGTGTCGCGCTCGAGCTTCCTCGGTCTCGCCTTCAAGCTCGTGCAGGAAGCCGAGAAATCCTGGCGGCGGATTCGCGGCGT
TGAGCGCATTGCCGAACTGATGGGCGGCGTCGTCTTCAAGGACGGCGAACCCGTGAAAGACGAAGAGCACGAACAGCAGCGGCTCGCCGCCTGATCAGCC
ATGCCGGATGGGTCCATACACCAGAATTGACTTTAGCTC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1275 bp424 aa1201394+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

LKKDTSEQSVEQSEVGLSLEELIRRGARDLIERAIEVEVQQLLAEYENVRMLGGSRAVVRNGYLPAREVLTAVGNVEVRVPKVRDRSGAGVKFNSALVPP
YVRRSARVSAALPWLYLKGISTGDMREALTVLLGDEAKGLSPNVVSRLKTEWASDYAGWMKRDLSGSRYVYWWADGVHTSLRGEDDARQCLLVVIGVRLD
GTKELVTVGDGHRESKASWLELLRDLKARGLEVGPRLAVGDGALGFWGALDELYPETRRQRCWVHKSANVLNALNALPKSLQAKAKAQLHEIWMAPTRAA
AVSAFERFVECYQAKYPKAADKLVRDRDALLAFYDFPAEHWIHLRTTNPIESTFATVRHRTTRTKNCVSRSSFLGLAFKLVQEAEKSWRRIRGVERIAEL
MGGVVFKDGEPVKDEEHEQQRLAA

 

Blast result :
Comments
ISAzo34 is 81% aa similar to IS1490.
References
1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.

2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.

3] PROSCIENCE (2004) Direct Submission GenBank.

4] ISfinder annotation (2008).