ISfinder Mirror Site

ISAcma28

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_009925	ND	Acaryochloris marina	Acaryochloris marina MBIC11017

DNA section

IS Length : 1130 bp

Ends

IR Length : 20/30

IRL : CCAATTCCATATATGTGTGACTTGTATTATGGGAGTAGCGCGTTGCTGAT
IRR : CCAATTCACTTATTGAGTGACCTTTATAGTCAAAGCATCCAAGATATAAG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CCACCATCAAACCCAATCGAGTGA	TA	TGACACCGAGGATTGCCGACTTTA	2
TTAACTCAGGCTATTCATGACTTA	TA	TTACGACTACTTTGACAAAGCATG	2
AGCGTCCCTTGCAGACCCTTGTCA	TA	TATCAGGTTACAACACGACACACA	2
GGGTTGACCCAAGAAATTTTCTAA	TA	TGACGCAGCATCAGAAACAGGTTG	2
AGTCAGCTTTCTTCTTCTGAGTAA	TA	TAAGACCCATGAAACTCTAAACGA	2

DNA sequence

CCAATTCCATATATGTGTGACTTGTATTATGGGAGTAGCGCGTTGCTGATACTTATCCCTCATGCCCAGACCCTTACGAATTGATATCTGTGAAAGTGAA
GACACACTCAAACGTTTATTACGTCAACAATCCTCCGGTCGTCGCAAAGAACGCTTGCAAGCCCTCTACTGGCTCAAAAGTGGTCAAGTCACCACTCGCA
TGGAGTTGGCAACCCTGACCGGACGAGGGGAATCCACGGTGTATCGCTGGATCAAACTCTATAAATCAGGGGGATTACAGGCAATGCTGGAGATTCCTAC
CTCTCCTGGGAAGCCCCCCATGATTGGTGGAGAGGTCCTCGAAAAGCTGAAAGCTCGTCTAGCTGACCCCAGGGGCTTTGAGAGCTACGGGGCGATTCAA
CAATGGCTTTTAGATGAGTGTGGATTAGACGTACCCTACAAAACGGTTCATCAAACCGTGCATTATCGGCTCAAAGCCAAGCTCAAAGTTCCTCGACCTC
GCTCACGCAAGACAGAGGAAGCTCAACAACAAGCCTATTAAAAAACTTCCTCGTGTGATTGAGGTAATGACCCGCTATTTTGCCCAAGGACACCCAGTGC
GCTACTGGTGCATGGATGAAAGCCGCTGGGGACTCAAGACACTCACGGGTCGGGTTCTAACGTTGCGGGGTGTCAAACCTGTGGTGACGGTACAGTGGCC
CCGAGAGGCTTTCTGGCTCTATGGCGCTGTCGAACCTCTCACAGGAGACAGTTTCTTCTATCGGTTTTCTCATCTCGATAGTGTTTGTTTTGAGCGCTTC
CTGGAGCAATTCAGCTTGGCGTTTCCCCAGAGTCTCAATCTGATGCAAATAGATCAAGCCGCAGCCCATATGGCGACTTGGGTTCGATGGCCTGAGAATG
TGTTGCCGATTGTACAGCCGTCTCATAGTCCAGAACTCAATCCGGCAGAGCGATTATGGCAGGACCTAAGAAAGCCTTTCAAAGGCAAAAACTTTGATTC
TATTGGGGCACTGGAGACCGCTTTATTTGAGCATATCAATACCCTCTCCCAGAAGGTGGTGGCTTCTCTAACGGGATATTCTTATATCTTGGATGCTTTG
ACTATAAAGGTCACTCAATAAGTGAATTGG

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
480 bp	159 aa	62	541	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MPRPLRIDICESEDTLKRLLRQQSSGRRKERLQALYWLKSGQVTTRMELATLTGRGESTVYRWIKLYKSGGLQAMLEIPTSPGKPPMIGGEVLEKLKARL
ADPRGFESYGAIQQWLLDECGLDVPYKTVHQTVHYRLKAKLKVPRPRSRKTEEAQQQAY

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
555 bp	184 aa	567	1121	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

MTRYFAQGHPVRYWCMDESRWGLKTLTGRVLTLRGVKPVVTVQWPREAFWLYGAVEPLTGDSFFYRFSHLDSVCFERFLEQFSLAFPQSLNLMQIDQAAA
HMATWVRWPENVLPIVQPSHSPELNPAERLWQDLRKPFKGKNFDSIGALETALFEHINTLSQKVVASLTGYSYILDALTIKVTQ

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1060 bp	349 aa	62	1121	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPRPLRIDICESEDTLKRLLRQQSSGRRKERLQALYWLKSGQVTTRMELATLTGRGESTVYRWIKLYKSGGLQAMLEIPTSPGKPPMIGGEVLEKLKARL
ADPRGFESYGAIQQWLLDECGLDVPYKTVHQTVHYRLKAKLKVPRPRSRKTEEAQQQAYKKLPRVIEVMTRYFAQGHPVRYWCMDESRWGLKTLTGRVLT
LRGVKPVVTVQWPREAFWLYGAVEPLTGDSFFYRFSHLDSVCFERFLEQFSLAFPQSLNLMQIDQAAAHMATWVRWPENVLPIVQPSHSPELNPAERLWQ
DLRKPFKGKNFDSIGALETALFEHINTLSQKVVASLTGYSYILDALTIKVTQ

Blast result :

Comments

ISAcma28 is 50% aa similar to ISSpn2. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.

References

1] Swingley,W.D., Chen,M., Cheung,P.C., Conrad,A.L., Dejesa,L.C.,Hao,J., Honchak,B.M., Karbach,L.E., Kurdoglu,A., Lahiri,S.,Mastrian,S.D., Miyashita,H., Page,L., Ramakrishna,P., Satoh,S., Sattley,W.M., Shimada,Y., Taylor,H.L., Tomo,T., Tsuchiya,T., Wang,Z.T., Raymond,J., Mimuro,M., Blankenship,R.E. and Touchman,J.W. (2008) Proc. Natl. Acad. Sci. U.S.A.
2] ISfinder annotation (2009)