ISAli10

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
FQ311874 ND Azospirillum lipoferum
Azospirillum lipoferum plasmid p6AZO533
DNA section
IS Length : 2584 bp

Ends


IR Length : 25/31

IRL : GTACGCCTCCGGTGAGTGCCGCCTTGGCAAAGTAGGGTCCGGCGTCTGAG
IRR : GTACGCATCCGGTGAATGCCGCGGTTAGGCAGCTTTCGTCTTCTCGCGGA

Insertion site


Left flankDirect repeatRight flankDR Length
TCAGTCCGCCGCGAGAGCGGCCAATGGCCTG8
CTGCTCACGCGACCAGGCCACATATCCTC8

DNA sequence

GTACGCCTCCGGTGAGTGCCGCCTTGGCAAAGTAGGGTCCGGCGTCTGAGTTTTGCGTTGCGCGTCATCGCGCGGACGCAAACGAGTTGCAAACGTCATG
GCTTACCAGCGTATCGAGGTTTTGACGGGCCGGGAACGGCGGCGGAGCTACACGCCGGCGGAGAAGGTGCGGATGGTTGAGGAGGCGTTCCGGCCCGGCG
TGGTGGTGACGGAGGCGGCCCGTCGGCTGGGCGTGCACGAAAGCCTGCTGTATCGCTGGCGGGACCTGATGAAGGTCGGCGGGACCTCCGTGGCCGAACC
GCCCAGCTTTGTCGCGGTGACCATCGCGCCGGAGCCGAGCGTGATGGAACCGCCGGTCGCGGAGCCTCATACGCCATTGCCGCCTCCGGCGGCGCCGGAG
ATGAGCCCAGCGATCCTCGAGGTCATCCTGCCCAGCGGGGCACGTCTGCGTCTGGAAGGGCCGGTCGACCCGGCCCTGGCGGCGGCCGTTGTCGGTGCCC
TGGCATGATTCCGGTGCCGAGTGGCGTGCGGGTGTGGCTGGCCAGCGGGCACACCGACATGCGCAAGGGCTGGGCAAGCTTGGCGCTGCTGGTGCAGGAA
CGCTTCGCCCAAGACCCGCACAGCGGTCATCTTTTCATTTTCCGCGGGCGCCGGGGCGATCTGGTGAAGATCATCTGGTATGACGGCCAGGGCAGTTGCC
TGTTCATGAAGAGGCTGGAGCGGGGCCGGTTCATCTGGCCGAGGCCGGCGGACGGTGCGGTGTCCATCTCGGTTGGACAGATGGGCTATCTGCTCGAGGG
CATCGACTGGCGCAACCCGCAGAAGACCTGGCGCCCCGAGGCCGCGGGGTGACTGCGACACGGTGAATCGACGCACCGGTTCAGGGGCGATTGCGGCGGC
TCAACGGCGCGTTTCCAGGTAAACTGGCGCCATGACCGCCGCCCCGCCCGACGACATTGCCAGCCTGCGCGCCGCCTTGGCGCAGGCCGAGGCGCGGGCG
GACGCGGCGGAAGCCGACGCGGCCCGGGCCAGGGCGATGGCATCCAACACCGAGGCGCTGATCGCCAGCCTGAAGCTGGAAATCGAGAAGCTCCGGCGCG
AGCTCTACGGCACGCGTTCCGAGCGCAAGGCGCGTCTTCTGGACCAGTTGGAGTTCCAACTCGAAGAGCTGGAAGCGACGGCCAGCGAGGACGAGCTGGC
GGCCGAGCAGGCTGCGGCCAAAACTACCGCGGTGGCGGCCTTCACCCGCAAGCGGCCGTCGCGCCAGCCCTTTCCCGAGCATCTGCCGCGCGAGCGCGTC
GTTGTGCCGGCCCCGGCGAGCTGCCCGTGCTGCGGCTCGGACAAGCTGTGCCGGCTGGGCGAAACGATCACCGAGACGCTGGAGGTGATCCCGCGCCAGT
GGAAGGTGATCCAGACGGTGCGCGAGCGGTTCTCTTGCCGGGCCTGCGAGACGATCAGCCAGCCACCGGCGCCGTTCCACGCCACCCCGCGCGGCTGGGC
CGGCCCCAACCTGCTGGCCACCCTGCTGTTCGAGAAGTTCGGCCAGCATCAGCCGCTGAACCGGCAGGCCGAACGCTTCGCACGGGAGGGTGTGCCGCTC
AGCCTGTCCACCCTGGCCGACCAAGTGGGCACCGCCGCGGCGGTGCTGAAGCCGCTGCACGACCTGATCGCCGCGCATGTACTGGCAGCCGAGCGGCTGC
ATGGGGACGACACGCCGGTGCCCGTGCTGGCCAAGGGCAAGACAGACACCGGGCGGTTGTGGGTGTATGTGCGTGATGACCGGCCATTTGCCGGCCAAGC
CCCACCGGCAGCGCTGTTCCACTATTCGCGAGACCGCAAGGGCGAACATCCCGAACGGCATCTGGCCGGCTTCACAGGCTGGCTGCAGGCCGATGCGTTC
GCCGGCTATAACCGGCTGTACGAACCCGACCGCCAGCCGGGGCCGATCAGCGACGTGCTGTGCTGGGCGCATGCCCGGCGCGGCTTCTTCAAGCTGGCCG
ACATCGCCGCGAACACCAGACGTGGCAAGGACGCCCCGCCGATCTCACCGCTGGCCCTGGAAGCCGTAAGGCGCATCGACGCCCTCTTCGATCTCGAGCG
CGCCCTGAACGGCAAGCCAGCGGCCGAGCGGCTGGCGGCCCGCCGGGAACACGGAGTCACCCTGGTAGCCGCGCTGGAGGACTGGATGCGGACGGAACGC
GCCCGGCTCTCCCGCCATGCCCCCGTGGCCAAGGCGATGGACTACATGCTGACCCGCTGGGACGGCTTCACCAAGTTCCTCAGCGACGGCCGGCTGTGCC
TGACAAACAACGCAGCCGAACGTAGCCTGCGCGGGATCGCTTTGGGCAGAAAAGCGTGGCTGTTCTGCGGCTCCGATCGCGGCGGGCAGCGAGCGGCGAT
CATGTACGGCCTGATCATCACGGCGAAGCTCAACGACGTCGATCCCCAGGCGTGGCTCGCCGACGTGCTCGCCCGCATCAACGACATCCCCCAGACCCGC
CTGCACAAACTACTGCCCTGGAACTGGAAGGCAATCCGCGAGAAGACGAAAGCTGCCTAACCGCGGCATTCACCGGATGCGTAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
411 bp136 aa98508+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MAYQRIEVLTGRERRRSYTPAEKVRMVEEAFRPGVVVTEAARRLGVHESLLYRWRDLMKVGGTSVAEPPSFVAVTIAPEPSVMEPPVAEPHTPLPPPAAP
EMSPAILEVILPSGARLRLEGPVDPALAAAVVGALA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
348 bp115 aa505852+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MIPVPSGVRVWLASGHTDMRKGWASLALLVQERFAQDPHSGHLFIFRGRRGDLVKIIWYDGQGSCLFMKRLERGRFIWPRPADGAVSISVGQMGYLLEGI
DWRNPQKTWRPEAAG

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1629 bp542 aa9322560+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTAAPPDDIASLRAALAQAEARADAAEADAARARAMASNTEALIASLKLEIEKLRRELYGTRSERKARLLDQLEFQLEELEATASEDELAAEQAAAKTTA
VAAFTRKRPSRQPFPEHLPRERVVVPAPASCPCCGSDKLCRLGETITETLEVIPRQWKVIQTVRERFSCRACETISQPPAPFHATPRGWAGPNLLATLLF
EKFGQHQPLNRQAERFAREGVPLSLSTLADQVGTAAAVLKPLHDLIAAHVLAAERLHGDDTPVPVLAKGKTDTGRLWVYVRDDRPFAGQAPPAALFHYSR
DRKGEHPERHLAGFTGWLQADAFAGYNRLYEPDRQPGPISDVLCWAHARRGFFKLADIAANTRRGKDAPPISPLALEAVRRIDALFDLERALNGKPAAER
LAARREHGVTLVAALEDWMRTERARLSRHAPVAKAMDYMLTRWDGFTKFLSDGRLCLTNNAAERSLRGIALGRKAWLFCGSDRGGQRAAIMYGLIITAKL
NDVDPQAWLADVLARINDIPQTRLHKLLPWNWKAIREKTKAA

 

Blast result :
Comments
ISAli10 is 48% (ORFA), 84% (ORFB) aa similar to IS66 and 59% (ORFC) to ISPre3.
References
1] ISfinder annotation (2008)