ISAzba7
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Azospirillum brasilense | Azospirillum brasilense |
DNA section
IS Length : 852 bp
Ends
IR Length : 15/17
IRL : GAGTGCGTAACAAAACCGGCCAGGAACGGTTGGTAGGGGCATGGCAGCCC
IRR : GAGCGCCTAACAGAACCATCTTTGGACGAAGCGCCAGCAGATGAGGCTGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTATGCCTTATAA | TTAGGCACTCTAA | 0 | |
| TGGAACGGCAGTTGGGT | TAA | GAGTGCAGATCAAAACC | 3 |
| GTTCCTTCCGATGAGCATAA | TGAAACGCTCTCAGGGGAGT | 0 |
DNA sequence
GAGTGCGTAACAAAACCGGCCAGGAACGGTTGGTAGGGGCATGGCAGCCCCTCTTGTTTCCGACGCCTTGTGGGCGATCATCGAACCGCTGATCCCGCCG
GAGCCGCCCAAGCCAAAAGGCGGACGGCCCCGGTTGGACGATCGTGCGGCGCTGACCGGCATCCTGTTCGTGCTGCGCACGGGCATCCCTTGGGAGCTTC
TGCCGGTGGAGATGGGCTGCGGCTCGGGGATGACCTGCTGGCGGCGTCTGCACGAGTGGCATCGGGCCGGGGTATGGGAGCGACTGCATCGCGTGCTGCT
CGACCGGCTCGGCTATGCCAACGCCATCAACTGGGATCGGGCGGCGGTGGACAGCGCCAGCGTTCCAGCAAAAAGGGGGGCGAGGAGACTGGCCCGAACC
CGACGGATCGCGGCAAGCCGGGCTCCAAGCGTCACATCCTCGTCGATGCCAATGGCATCCCACTCGCCCTGAAGATTTCGCCGGCCAACCGGCACGATAG
CAAGCTGCTGGAGACGCTGGTCGATGCCGTGCCGGCGATCCGCCAGTGTGCGGGCCGGCCACGGCGCCGCCCGGCCAAGTTGCACGCCGACAAGGGCTAC
GACTTTGCACACTGCCGGCGGGCGCTGCGCCAACGAGCGATCATTCCGCGGATCGCCCGGCGTGGCGTCGAGAGCAGCGAGCGTCTTGGCCGACACCGAT
GGGTGGTCGAGCGGACGCTCGCATGGTTCGCCCGCTTCCGCCGCATCGCCGTCCGATACGAACGGCGCGCCGACATCTTCACCGCCTTTCACCACATCGC
CGCCAGCCTCATCTGCTGGCGCTTCGTCCAAAGATGGTTCTGTTAGGCGCTC
GAGCCGCCCAAGCCAAAAGGCGGACGGCCCCGGTTGGACGATCGTGCGGCGCTGACCGGCATCCTGTTCGTGCTGCGCACGGGCATCCCTTGGGAGCTTC
TGCCGGTGGAGATGGGCTGCGGCTCGGGGATGACCTGCTGGCGGCGTCTGCACGAGTGGCATCGGGCCGGGGTATGGGAGCGACTGCATCGCGTGCTGCT
CGACCGGCTCGGCTATGCCAACGCCATCAACTGGGATCGGGCGGCGGTGGACAGCGCCAGCGTTCCAGCAAAAAGGGGGGCGAGGAGACTGGCCCGAACC
CGACGGATCGCGGCAAGCCGGGCTCCAAGCGTCACATCCTCGTCGATGCCAATGGCATCCCACTCGCCCTGAAGATTTCGCCGGCCAACCGGCACGATAG
CAAGCTGCTGGAGACGCTGGTCGATGCCGTGCCGGCGATCCGCCAGTGTGCGGGCCGGCCACGGCGCCGCCCGGCCAAGTTGCACGCCGACAAGGGCTAC
GACTTTGCACACTGCCGGCGGGCGCTGCGCCAACGAGCGATCATTCCGCGGATCGCCCGGCGTGGCGTCGAGAGCAGCGAGCGTCTTGGCCGACACCGAT
GGGTGGTCGAGCGGACGCTCGCATGGTTCGCCCGCTTCCGCCGCATCGCCGTCCGATACGAACGGCGCGCCGACATCTTCACCGCCTTTCACCACATCGC
CGCCAGCCTCATCTGCTGGCGCTTCGTCCAAAGATGGTTCTGTTAGGCGCTC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 432 bp | 143 aa | 41 | 472 | + | No |
Description : First part of the transposase
ORF sequence :
MAAPLVSDALWAIIEPLIPPEPPKPKGGRPRLDDRAALTGILFVLRTGIPWELLPVEMGCGSGMTCWRRLHEWHRAGVWERLHRVLLDRLGYANAINWDR
AAVDSASVPAKRGARRLARTRRIAASRAPSVTSSSMPMASHSP
AAVDSASVPAKRGARRLARTRRIAASRAPSVTSSSMPMASHSP
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 777 bp | 258 aa | 70 | 846 | + | No |
Description : Second part of the transposase
ORF sequence :
VGDHRTADPAGAAQAKRRTAPVGRSCGADRHPVRAAHGHPLGASAGGDGLRLGDDLLAASARVASGRGMGATASRAARPARLCQRHQLGSGGGGQRQRSS
KKGGEETGPNPTDRGKPGSKRHILVDANGIPLALKISPANRHDSKLLETLVDAVPAIRQCAGRPRRRPAKLHADKGYDFAHCRRALRQRAIIPRIARRGV
ESSERLGRHRWVVERTLAWFARFRRIAVRYERRADIFTAFHHIAASLICWRFVQRWFC
KKGGEETGPNPTDRGKPGSKRHILVDANGIPLALKISPANRHDSKLLETLVDAVPAIRQCAGRPRRRPAKLHADKGYDFAHCRRALRQRAIIPRIARRGV
ESSERLGRHRWVVERTLAWFARFRRIAVRYERRADIFTAFHHIAASLICWRFVQRWFC
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 806 bp | 268 aa | 41 | 846 | + | Yes |
Chemistry : DDE
ORF sequence :
MAAPLVSDALWAIIEPLIPPEPPKPKGGRPRLDDRAALTGILFVLRTGIPWELLPVEMGCGSGMTCWRRLHEWHRAGVWERLHRVLLDRLGYANAINWDR
AAVDSASVPAKKGGEETGPNPTDRGKPGSKRHILVDANGIPLALKISPANRHDSKLLETLVDAVPAIRQCAGRPRRRPAKLHADKGYDFAHCRRALRQRA
IIPRIARRGVESSERLGRHRWVVERTLAWFARFRRIAVRYERRADIFTAFHHIAASLICWRFVQRWFC
AAVDSASVPAKKGGEETGPNPTDRGKPGSKRHILVDANGIPLALKISPANRHDSKLLETLVDAVPAIRQCAGRPRRRPAKLHADKGYDFAHCRRALRQRA
IIPRIARRGVESSERLGRHRWVVERTLAWFARFRRIAVRYERRADIFTAFHHIAASLICWRFVQRWFC
Blast result :
Comments
ISAzba7 is 77% aa similar to ISMch2. The tird ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2009)