ISAzo1

  • Family IS1182
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_006513 ND Azoarcus sp.
Azoarcus sp. EbN1
DNA section
IS Length : 1545 bp

Ends


IR Length : 14

IRL : CAGCGTGTCGGACTATCCCCTGTAAAATGACGCGTCGCCCCTGGAACCCG
IRR : CAGCGTGTCGGACTTGAGCACCGCCGGCGCTGTATCCGGGGGCTTGATTT

Insertion site


Left flankDirect repeatRight flankDR Length
NNNNNNNNNNCTAGNNNNNNNNNN4

DNA sequence

CAGCGTGTCGGACTATCCCCTGTAAAATGACGCGTCGCCCCTGGAACCCGAGAGCATGAGCCGCTTCCGCCCGATCGATCGCCAAACGGACTACCTGCTA
CCACCGTCGGTGCAGGACTGGCTGCCCGAATCGCACTTGGCGCGCTACGTGGTCGATGTGGTGGAAGGGCTGGACCTGTCGGCATTGGAGCGCGCCTACG
CGGGTCGCGGTAGCGATGCCTACCACCCGGCGCTGCTGCTGTCGCTGCTGATCTACGGCTACGCGACGGGAACCCATTCGAGCCGCAAGATCGAGCGGGC
CACGTACGACTCGCTGGCCTTCCGCTTCATCGCGTGCGACCAGCATCCGGACCACGACACGCTGGCGACGTTCCGCCGCCGTTTCGGCGATCAGTTCGCC
GATGCCTTCGTGCAGGTGCTGCAAGTCGCCCGCGAGAACCAGCTCTCGCGCTTTGGCACCGTGAGCCTGGACGGTACCAAGATCCACGCCAACGCCAGCC
GACACAGCGCGCTCTCGTATGGGCACGCCGAGAAGATCGAGGCCCAGCTCAAGGCCGAAGTGCAGGAGATGCTCGCGCTGGCCGAAGCGGCCGATCGGAG
CTGCGTGCCCGAGGGCGTGGATCTGCCCGCGGAGATCCAGCGCCGAGAAGACCGGCTGGCCGCCATCGCGGCCGCCAAGGCCAAGATCGAAGCGCGCGCC
AAGGAGCGCTTCGAGCGCGAACAGGCCGAGTTTGATGCCAAGCTGGCCAAGCGTCAGGCCAAGGCGGCGGCCACCGGCAAGAAGCCCGGCGGCAAGCCGC
CGACCCCGCCTGCCCCCGGTCCGCGCGCGGACGACCAGCTCAATCTGACCGACGAAGACTCGCGCATCATGAAAGTGACCGGCGGCGGCTTCGAGCAGTG
CTACAACGCGCAGGCGCTGGTCGATACGGAGTCGATGCTGGTGATGGTGCCCCACCTTACCCAGGCGGGCAACGACAAGGAGCAGGTCGAGCCGATGCTG
GCCCGCATCGCGGCCCTGCCCGAAGGGCTCAATCAGCCCGACCAACTGCTGGCCGACACCGGTTTCTTCAGCGAGCGCAACGTCGAGCGCTGCCAGGCCG
CCGGGATCGAACCGCTGATCGCGGTCGGGCGCGACGAGCACCATCCGGATTGGCGCCGCCGCTTCGAAGAGCCCGCCCCGCTCGAGCAGCCCGCCAGCCC
CGTCGAACAGATGAAGCACGCCCTCAAGACCCGGGCCGGCCGCGCCGCCTACGCGCTCCGGAAACAGACCGTGGAACCGGTGTTCGGCATCATCAAGTCG
GTGATGGGATTTCGCCAGTTCCTGCTGCGCGGGCTCGACAACGTGCGCGCCGAGTGGACCCTGGTGTGCTTGGCGTGGAACTTGAAGCGCATGGCCGTAT
TGCGTCCGCAGTGAGAAAAAAGGACAGGGGCATTGCGATTTCGGACAGGAAACCGCTGATTGGAACCTGAAATGCCGACCGTTTCTCACATTGTGAAATC
AAGCCCCCGGATACAGCGCCGGCGGTGCTCAAGTCCGACACGCTG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1359 bp452 aa561414+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSRFRPIDRQTDYLLPPSVQDWLPESHLARYVVDVVEGLDLSALERAYAGRGSDAYHPALLLSLLIYGYATGTHSSRKIERATYDSLAFRFIACDQHPDH
DTLATFRRRFGDQFADAFVQVLQVARENQLSRFGTVSLDGTKIHANASRHSALSYGHAEKIEAQLKAEVQEMLALAEAADRSCVPEGVDLPAEIQRREDR
LAAIAAAKAKIEARAKERFEREQAEFDAKLAKRQAKAAATGKKPGGKPPTPPAPGPRADDQLNLTDEDSRIMKVTGGGFEQCYNAQALVDTESMLVMVPH
LTQAGNDKEQVEPMLARIAALPEGLNQPDQLLADTGFFSERNVERCQAAGIEPLIAVGRDEHHPDWRRRFEEPAPLEQPASPVEQMKHALKTRAGRAAYA
LRKQTVEPVFGIIKSVMGFRQFLLRGLDNVRAEWTLVCLAWNLKRMAVLRPQ

 

Blast result :
Comments
ISAzo1 is 40% aa similar to ISPsy6.
There are 5 copies in Azoarcus sp. EbN1 genome and one copy in plasmid 2.
References
1] ISfinder annotation (2005)
2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36
3] Kube,M., Heider,J., Amann,J., Hufnagel,P., Kuhner,S., Beck,A., Reinhardt,R. and Rabus,R.(2004) Arch. Microbiol. 181 (3), 182-194
4] Rabus,R., Kube,M., Beck,A., Widdel,F. and Reinhardt,R. (2002) Arch. Microbiol. 178 (6), 506-516