ISfinder Mirror Site

ISAmu1

Family IS1595
Group ISNwi1

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_010655	ND	Akkermansia muciniphila	Akkermansia muciniphila ATCC BAA-835

DNA section

IS Length : 2422 bp

Ends

IR Length : 18/21

IRL : GGGATGATTCCAATGCTGGAAAATCATCTCCCGGGCCTGGAGGCGGTTAT
IRR : GGTATGATTCCAATTTTGGAATGAGCATTAGAGGAATGTACGGATTCCAT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TGCAAATGGA		GTGTTGAATA	0

DNA sequence

GGGATGATTCCAATGCTGGAAAATCATCTCCCGGGCCTGGAGGCGGTTATTCTCCCGGAACTCCGGCGTATCCCACACCAGGTTGACATTGGGGACCTCG
GACCAGACAATCAGCCCCGCCTTGTCGCACAGGTCGTAAATATTGGTGGATGCGGGATAGTGGCCCGTTCTAACCCCATCCGCCCCCATATCCGCAATCA
GCCTGATATCCCGCGCCTCATCTTCCGGGGAAAGGGCCCACCCCTTCCCCTTCATATCCTGATGGCGGCTGACCCCCTTGATCTGCATGGTCTTTCCGTT
CAGGACAAAACCTTCCTTGGGATTGACGGAAACCGTCCTCAGGCCCAGCGGCTGCACCAGCTCATCCGTCTGGCCATCCGCCGTCCTGATGGAAGTTTTC
ACCTGGTACAGGTAAGGATTCTTCACTCCGTTCCAGAGTACGGGATTGGCGATTGCCAGCGTTACGGGAACCTCCAGTCTATTCTTTTCCCCGGCGGCGG
CCTTTACCGTCTTCCCGGCTACCTTCCTGCCCTTCATGTCCAGGATTTCCACGGCAACATCCACATCCCCGGCCTTTCCGTCCGAATTGAGAAGGGTCTT
CACTTCCACCTCGGCCTTGGATTTGCTTAAAGACTTTGTGGTGATGAAAACGCCGGGAGAAGCATAAAAAAGAGGATCAATGCAGACACCGTCCGTCTCA
ATCAGTTCAATGGGCCGGTACAGCCCGCCGAACATGGAAAAATCCCCGCGCTGGGGGGCGATATGATTACGCTGCGTGTTGTCCACCAGAACGGAAATCG
TATTGGAGCCTTTTTTCAAAGGGCCTTATATACTTGACTTCGGTTTCTCCGTATGCTATTAATTAGCCATACTAAGGAGGCACCCATGAACGACCGCTTA
ACTATTTCACTTTTTGAGCTTTTTGAAAAATTCCCCAATGAGGAATCAGCTAAAAAGTATTTAGAGGAAAAGCGGTGGGGCGATAAGGTAGTTTGCCCGT
TCTGCGGGAAAAGCGAAAAACAGTATAGAGTAAAGAGGAACGGGGTTGAGGGGTATTTTGAATGCGGGGAGTGCGGGAAAGTTTATACTGTGAGGACTGG
AACTATTTTTGAGAGAAGCCATGTTCCACTTCATAAATGGATTTTTGCCTTCTATTTAGTAGTGACTTCCAGAAAGGGTATTAGCTCCATGCAACTTTCC
AAGGAAATTGGGGTTACGCAAAAAACGGCGTGGTTCATGCTTCAAAGGATTAGGGAAGCATGCAATTCCAACCATGATGACGATGACAATAACGGCTTTC
TATCTGGAATTGTTGAAGCTGATGGGGTGTATATTGGAGGCAAGGAATCCAATAAGCACGAATCTCAGAAATTGAAGGCTGGGCGCGGAATTGTTGGAAA
GACAGCGGTTTTAGGAATGAGAGCCAGAGCAGGAAAGGTTTTAGCAAGGGTTATTCCCGACACTTCAAGAGATACAGTTCACCAAGTTTTGAATAAGTAT
TTGAGCCGGGATTCCGTTCTGGTTACTGATGAACATGCTTCTTACAAAGAATCACAGTTCAATCATCAGGTGGTGAATCATAGTGCCAAGCAGTATGTTG
ACGGAATGGCCCATACTAACGGCATCGAGAGCGTATGGGCGGTTTTGAAGAGAGGATTCTATGGAATCTACCATTCATTCAGCACAAAACATTTACAGAG
ATATGTAGATGAATTTGCGTATCGGTTGAATGAGGGAAATGTGAAAATCCACACATGGGTAAGATTGGATTCTCTTATGGCTAAAGCATTTTCGACGAGA
ATTACTTACGCCGAATTGAAGAATTGACCGCCACCTTGCGAGCAAGGCGACGGCCTAAGTCATGCAAATCAAAGAATCGCCCCCTTCCCCGAAAGGAAGG
AGGCCAGTTAATTCACCGCCGAAAGTTAGAATGACCAGCTAAAGCCAACTGTGATAAAGTGGTATGGGTCAACATCTGACCAGTATTTCGTTATTTCATA
TCCAATAACGATGTCTGTTTTTTCTGCAACAGCAAATTTGAAACCGATTAATGCCGAGTAAGTTCCACTAGAATCAGACTCAGATACTTTTCCTCCACCT
CCAGAGAGTTCCTGTTTATAATAATGAAGGCCTATTTTGCCGCCTAAATAGAAAGTGGCAGAATCAGATAAGGGGACATTAAAAGAATATCCAGCGGTAA
ATGGGAATACCTGTTGTTTCCATTTCAACTCCCCTTCGTAGGTTTTAGAACCTGTAAGCGCGCCGAGATTGAAAGATAATTCATGGAAAACAGAACCCGC
TGGAATGTTAGCGTGAAGTCCTAATTGGGCACCGTACAGGTCTGGCATATCTATTCCATAATCATTCCCCGAATGGAATCCGTACATTCCTCTAATGCTC
ATTCCAAAATTGGAATCATACC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
942 bp	313 aa	886	1827	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNDRLTISLFELFEKFPNEESAKKYLEEKRWGDKVVCPFCGKSEKQYRVKRNGVEGYFECGECGKVYTVRTGTIFERSHVPLHKWIFAFYLVVTSRKGIS
SMQLSKEIGVTQKTAWFMLQRIREACNSNHDDDDNNGFLSGIVEADGVYIGGKESNKHESQKLKAGRGIVGKTAVLGMRARAGKVLARVIPDTSRDTVHQ
VLNKYLSRDSVLVTDEHASYKESQFNHQVVNHSAKQYVDGMAHTNGIESVWAVLKRGFYGIYHSFSTKHLQRYVDEFAYRLNEGNVKIHTWVRLDSLMAK
AFSTRITYAELKN

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
474 bp	157 aa	2399	1926	+	No

ORF function : Passenger Gene
Annotation : Description :

ORF sequence :

Blast result :

Comments

ISAmu1 is 63% aa similar to ISRhba1. The second ORF is a passenger gene annotated as hypothetical protein. Before the transposase, there is a pseudogene annotated as a possible beta-galactosidase.

References

1] ISfinder annotation (2009)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L., Lang,D., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Smidt,H., Derrien,M., Plugge,C.M., Zoetendal,E.G., de Vos,W.M. and Richardson,P. (2008) Direct submission GenBank.