ISAcp1

  • Family IS5
  • Group IS5
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_012483 ND Acidobacterium capsulatum
Acidobacterium capsulatum ATCC 51196
DNA section
IS Length : 1483 bp

Ends


IR Length : 12/14

IRL : GTTGTTCCTGAAAAACCAGGAAAAGGTGCATAAAAACAGGAAAAGGCGGG
IRR : GTCGATCCTGAAAAGTGGATTTCAGGTTGTTAGGGATGAGATCGGCGGGT

Insertion site


Left flankDirect repeatRight flankDR Length
GATCGGTAGCTACGCTAACCATCTATT7

DNA sequence

GTTGTTCCTGAAAAACCAGGAAAAGGTGCATAAAAACAGGAAAAGGCGGGCAAACAGGGGCATAGAATTTCCCCATAAGAAGCAGTCGTGAGGGGAGAAG
CTGGGGAAAACGATGCGACCGAAGAGCGAGCCGAAGCAGGTGCAGCGAGAGTTGTTCCAGGTGGAGCTGGAGCAGATCATCGACATGAATCACGCCCTGG
TGCGGCTGGGACAGAGCATCGAATGGGCAGAGTTTGAGGAGGCGCTGGGAGCGACGTATCACCCGACCCAGGGAGCCCCCGGCATTTCCACGCGCCTGAT
GGTGGCACTGCATTATCTGAAGTACCAGCACGATCTGAGCGATGAAGGGGTGCTGGCGCATTGGGTGGAGAATCCCTACTGGCAGCATTTCAGCGGAGGG
CGGTACTTTCAGCATCGTCTGCCCATGGACGCGTCGAGCATGACGCGCTGGCGCAGTCGGCTGGGCGAGGCCGGAGCCGAACAGATGCTGCGCGCGACCA
TCACGGCGGGGATCAGGATGGGTGCGATTCGCAGCTCTGAGCTGAAGCGGATCAACGTGGATACGACGGTGCAGAGCAAGGCGATCCGTTATCCGACTGA
CGCGCGGTTGTATCACCGCTGCCGGGAGCGGCTGGTGAAGGCGGCGCGGCAGGAAGGAGTCAAGATCAAGCAGAGTTATCAGCATGTGGGCAGGAAGCTG
TTGATGCAATCCAGCCGCTATGCCCATGCCCGGCAGATGCAGCGGGCGCGGGCCTGCACACGCAAGCTGCGTACCCAGTTAGGACGGGTGATACGCGAGA
TAGAACGGCAAGTGACAGAGCCTTCCGATACGTTATCGAAGTTGCTGGAAACGGCGCATCAGATCCACGCCCAGCAAAGGCATGACAAGAACAAGGTGTA
CAGCGTGCATGAGCCCGAGGTGGCCTGCATCGCCAAGGGCAAGGCGGGCAAGCCGTATGAGTTTGGCAACAAGGTCAGTGTGGCTTCCACCAGCCGGGGC
GGATGGCTGGTAGGCGCAAAAAGCTTCACCGGCAATCCCTATGATGGTCACACGCTGGCCGCGCAGATGAAGCAGGTCAGGAGCATGATCGGCGAGCGGG
TGCGCGAGGCGCATGTGGACATGGGCTATCGCGGTCACAACTACCAGGGCCGGATTACGGTGCATTTGGACAAGCGAAGAAGAGGCCGCACGCCGCGACC
GCTGTGGCGCTGGATGAAACGCCGCGCCGCCATCGAGCCCAGCATCGGCCATCTGAAGAACGAACACCGACTGGACAGGAACCGCTTGAAGGGTGTTGCC
GGTGATGCGATCAACGCCCTGCTCGCGGCCGCAGCAATGAACTTCCAGAAGCTCCTCGGAGTTTTTTGGCTCTATTTTCTGCGCGCTCTGCTGAACGTGC
TGTCGCGATTGCGGCTCCTCACCGCGCCAACCAACCCGCCGATCTCATCCCTAACAACCTGAAATCCACTTTTCAGGATCGAC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1350 bp449 aa1131462+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRPKSEPKQVQRELFQVELEQIIDMNHALVRLGQSIEWAEFEEALGATYHPTQGAPGISTRLMVALHYLKYQHDLSDEGVLAHWVENPYWQHFSGGRYFQ
HRLPMDASSMTRWRSRLGEAGAEQMLRATITAGIRMGAIRSSELKRINVDTTVQSKAIRYPTDARLYHRCRERLVKAARQEGVKIKQSYQHVGRKLLMQS
SRYAHARQMQRARACTRKLRTQLGRVIREIERQVTEPSDTLSKLLETAHQIHAQQRHDKNKVYSVHEPEVACIAKGKAGKPYEFGNKVSVASTSRGGWLV
GAKSFTGNPYDGHTLAAQMKQVRSMIGERVREAHVDMGYRGHNYQGRITVHLDKRRRGRTPRPLWRWMKRRAAIEPSIGHLKNEHRLDRNRLKGVAGDAI
NALLAAAAMNFQKLLGVFWLYFLRALLNVLSRLRLLTAPTNPPISSLTT

 

Blast result :
Comments
ISAcp1 is 78% aa similar to ISOpb2.
References
1] ISfinder annotation (2010)
2] Ward,N.L., Challacombe,J.F., Janssen,P.H., Henrissat,B., Coutinho,P.M., Wu,M., Xie,G., Haft,D.H., Sait,M., Badger,J., Barabote,R.D., Bradley,B., Brettin,T.S., Brinkac,L.M., Bruce,D., Creasy,T., Daugherty,S.C., Davidsen,T.M., DeBoy,R.T., Detter,J.C., Dodson,R.J., Durkin,A.S., Ganapathy,A., Gwinn-Giglio,M., Han,C.S., Khouri,H., Kiss,H., Kothari,S.P., Madupu,R., Nelson,K.E., Nelson,W.C., Paulsen,I., Penn,K., Ren,Q., Rosovitz,M.J., Selengut,J.D., Shrivastava,S., Sullivan,S.A., Tapia,R., Thompson,L.S., Watkins,K.L., Yang,Q., Yu,C., Zafar,N., Zhou,L. and Kuske,C.R. (2009) Appl. Environ. Microbiol. 75 (7), 2046-2056