ISAme1

  • Family IS4
  • Group IS231
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Alkaliphilus metalliredigenes
Alkaliphilus metalliredigenes QYMF
DNA section
IS Length : 1488 bp

Ends


IR Length : 16/17

IRL : CATAGGCGTTAACTTAACCCATGTTATTCCAAGGTAAAAATTACATTTGA
IRR : CATAGGCGTTAACCTAAGCAATATTTTGGAAATTAATTTCGAGAAAAAAG

Insertion site


Left flankDirect repeatRight flankDR Length
ATTGGGGAATACTTGTTAAT0

DNA sequence

CATAGGCGTTAACTTAACCCATGTTATTCCAAGGTAAAAATTACATTTGAAAAAAAGCATGAAAAATGGGAATCTCAAAGTGACCATCACATCACAAAGG
AGAGATTCCCATGAAGATATCTTCATCACTTATTATACAATTTATTCGGTTGTTTGATAACAATAAAATTATGGAAATTGCCATAGGCACAGGTTTATTG
AAGCGTCAAAAGGGCATGTTGCCTGATACAATTCTTAAGGTTTTCACATTCGGGTTGCTAAATATAGCAAACCCCTCATTAAACCAAATTGCATCTAAAT
GCCAAGCATTTCAACCAGGCTTAACAATCTCCAAAGAAGCAGTGTATAAAAGACTGAAAAAATCTTCTTTATTTTTACAGGAAACATTTAAACATATGAT
GCAAAAATCTATGAATAGTGTAATCCCTGTAAAAACTGCCGCTATTCTTGAGCAGTTTAAGGATGTAAAGATATGTGATAGTACCAAAATTACTTTGCCG
GATAAGCTGGTGGCTCTGTACCCCGGATTAGGTGGACGCAATGCTAAATCTTCTTTAAAAGTCCAAGGCATTTATAGTTTGATTCCTGCTAGATTTTCAA
GCCTTGAAATAACAAAGGCTCCTGGCGCTGATACTACCTATAACGATAAGTTACTTGCCATGGTTAATCCAGGTGAATTGCTAATAACAGACCTAGGATA
TTTTAGCAAAGCATTTTTTGAAAAACTATCAACAAAAGGTAGTTACTATCTCACTAGAATCAAGAAAAACTCTATTGTTTACGTAGAAAAGTCAGGACAA
CTAACTAAGGTGGATTTGACTGATTTACTAAAGGGTACTGTGGTTGATACAGAAGTTTTTCTGGGAATAGCCCATAAAAAACAACTTAAATGTCGTTTTG
TAGCCATTCGCCTGCCAGAAAAAGTGGTGAATCAACGTAGACGCAAGGCTAACCAACAGGCTAAAGCCCAAGGCAAACAACTTAGTGCGAAAGAAACTGA
ATTGCTGGCATGGAATATTATTGTTACAAATGTTACAAAAGATAAGTTATCTCCTGAAGCTGCTTGTGATTTATATAGAGCAAGATGGCAAATTGAACTC
GTATTTAAATCATTAAAAAGCTATTTAAATATTGATAAAATAGGCTCTTGTGGTAAATACCAGCTAGAGTGTTTGATATATGGGCGCTTAATAGCAGTAG
TTGCCATGTTTTCTTTATACAATGTTCTATATATACCAGCAAATCAACATTTCACAAGAAGTTTAAGTATGTTGCGATTTGTAAGTATTTTTGCAATTCA
TGCTAATGAGATAGCCCTAAAGCTTCAATTAACAATACCAAATATTCATTTTCTTGAAAGACTTTTCAAGAAAATGAGTAAAAAAAGTTTGCATGATAAG
CGTCAGAGAAAAACAACGTTTGAAATACTACAGGAATACTTTTTTCTCGAAATTAATTTCCAAAATATTGCTTAGGTTAACGCCTATG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1362 bp454 aa1111472+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKISSSLIIQFIRLFDNNKIMEIAIGTGLLKRQKGMLPDTILKVFTFGLLNIANPSLNQIASKCQAFQPGLTISKEAVYKRLKKSSLFLQETFKHMMQKS
MNSVIPVKTAAILEQFKDVKICDSTKITLPDKLVALYPGLGGRNAKSSLKVQGIYSLIPARFSSLEITKAPGADTTYNDKLLAMVNPGELLITDLGYFSK
AFFEKLSTKGSYYLTRIKKNSIVYVEKSGQLTKVDLTDLLKGTVVDTEVFLGIAHKKQLKCRFVAIRLPEKVVNQRRRKANQQAKAQGKQLSAKETELLA
WNIIVTNVTKDKLSPEAACDLYRARWQIELVFKSLKSYLNIDKIGSCGKYQLECLIYGRLIAVVAMFSLYNVLYIPANQHFTRSLSMLRFVSIFAIHANE
IALKLQLTIPNIHFLERLFKKMSKKSLHDKRQRKTTFEILQEYFFLEINFQNIA

 

Blast result :
Comments
ISAme1 is 49% aa similar to IS231O. ISAme1 was found by screening completely sequenced genomes for sequences homologous to the ISPa12 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-70-D(N3)-134-E(C1). The sequencing of the Alkaliphilus metalliredigenes QYMF chromosome is currently in progress. Accession number: NZ_AAKU00000000.
References
1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina,T., Hammon,N., Israni,S., Pitluck,S. and Richardson,P.(2005)Unpublished