ISA1218

  • Family IS5
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_000917 ND Archaeoglobus fulgidus
Archaeoglobus fulgidus DSM 4304
DNA section
IS Length : 1218 bp

Ends


IR Length : 15/26

IRL : GGGTGTTCCTTCAACCCCTAACCCAATCTCCTTCCATTTACCTCTGTAAA
IRR : GGTCTTCTCTATATCACCACTCCGACAATCTCTGAATTACCTTCTTTTCC

Insertion site


Left flankDirect repeatRight flankDR Length
AAAGGTAAAGTTATATGTTTTGATT5

DNA sequence

GGGTGTTCCTTCAACCCCTAACCCAATCTCCTTCCATTTACCTCTGTAAAGCGAACAACGAGCTCTTCCACCTTCCCCTTCAACAGGACAAAACTACACA
ACCCTAAATCAAAAACAAAACCACCAAAATTCGAAAAATTAATCAATCTAACCGAATCATCCTTTTTCAGGTGATTCAAGTGAAACTACCAGTAATTCCT
GCCAGAGGAGACGAGAAATGGGAGCTCCTATCTCAAATCGTAAGCAAGCTTGAATCAAGAGAAGCAAAGAAATCGCTGGCGAGAAATGGAATATCTCCAG
TCAATAAAGCCGTAGAGTACCTCAAAGTAATGGCTATGTTCTTCGAACTGGAGATTTCCTATGCTGTAAGCGAATTGAATAAGCGGTCAGAACTGAGAAA
GTTCTTGAGGCTAGGAGAGGAAATAAAACTGAGATCTATCTACAGCTTCATGTCGAAATTTGAGGCTGAGCAGTTTATAAGCTTAGTTTTCTCCATCCTA
AACGTTAACGCAAAGAGAAGTAGAAAGAAATCATCAATTTTAATATTGGATTGGACAGATATATCACTCGACCTAAATCCCTTCAGGAGGAGAGAATTGA
GTAATAAGCCATACAAATGGGGTTATTCAACCAAGGGATTCTTTCTCGGCATGAAGATGATGATTTTAATCGACTACAATACTTTAACGCCTCTTTTCTT
CCACATTTACCCTGCTAACACTCACGAGAGCAGAATCTATCCGGTAATTCTGGAAATGCTCAAGAGGAGGAAGCTAATCAGGTTTGGAGACTCTATCATC
ATGGACAGAGGTTTCTACGCCTACAAAAACTATCTGATTGGACTGAGATACGGGATCATTCCCCTAATTATCCCAAGAAAGAATTTCAGAGAAGAGAGGC
TTAAAGGGATGGTTAGCTATCCTCTGAGCATATTTGCCTCTAAGAACGTTGAGAAGGAGAAAAAGAGATACAGGAAGCTTGTAAGAAAATTGTTTGAAGG
GTTGAAGCAAAATCTTAAGACTTTGAGGAGTATAATTGAAGACGTGATAAAGCTGGGGAAAGAAGCTTTCAGTTTGAGGGATTTGCACTGCTATACGGTC
GATTCTGTGAGAAAGAAGTGTGCTCTTTCTGTCCTGTTAGTGGGGATGACGGTTAAGCTGGGTTTTAGGGAAAAGAAGGTAATTCAGAGATTGTCGGAGT
GGTGATATAGAGAAGACC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1035 bp344 aa1711205+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MIQVKLPVIPARGDEKWELLSQIVSKLESREAKKSLARNGISPVNKAVEYLKVMAMFFELEISYAVSELNKRSELRKFLRLGEEIKLRSIYSFMSKFEAE
QFISLVFSILNVNAKRSRKKSSILILDWTDISLDLNPFRRRELSNKPYKWGYSTKGFFLGMKMMILIDYNTLTPLFFHIYPANTHESRIYPVILEMLKRR
KLIRFGDSIIMDRGFYAYKNYLIGLRYGIIPLIIPRKNFREERLKGMVSYPLSIFASKNVEKEKKRYRKLVRKLFEGLKQNLKTLRSIIEDVIKLGKEAF
SLRDLHCYTVDSVRKKCALSVLLVGMTVKLGFREKKVIQRLSEW

 

Blast result :
Comments
ISA1218 is 66% aa similar to ISMba15.
References
1] ISfinder annotation (2010)
2] Klenk,H.P., Clayton,R.A., Tomb,J.-F., White,O., Nelson,K.E., Ketchum,K.A., Dodson,R.J., Gwinn,M., Hickey,E.K., Peterson,J.D., Richardson,D.L., Kerlavage,A.R., Graham,D.E., Kyrpides,N.C., Fleischmann,R.D., Quackenbush,J., Lee,N.H., Sutton,G.G., Gill,S., Kirkness,E.F., Dougherty,B.A., McKenney,K., Adams,M.D., Loftus,B., Peterson,S., Reich,C.I., McNeil,L.K., Badger,J.H., Glodek,A., Zhou,L., Overbeek,R., Gocayne,J.D., Weidman,J.F., McDonald,L., Utterback,T., Cotton,M.D., Spriggs,T., Artiach,P., Kaine,B.P., Sykes,S.M., Sadow,P.W., D'Andrea,K.P., Bowman,C., Fujii,C., Garland,S.A., Mason,T.M., Olsen,G.J., Fraser,C.M., Smith,H.O., Woese,C.R. and Venter,J.C. (1997) Nature 390 (6658), 364-370.