ISAzs8

  • Family ISL3
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_013854 ND Azospirillum sp.
Azospirillum sp. B510
DNA section
IS Length : 1373 bp

Ends


IR Length : 9/11

IRL : GGCGGCAACCCTGGCGCCCGTCATGCCACGGCGATGGGAATGCCGATCAG
IRR : GGCGACATCCCAATTTGGGCTCTTGGAAATTTTGTGTGGATGAGTAGAAG

Insertion site


Left flankDirect repeatRight flankDR Length
CCGGCTGTAGTACTATGACAGGAACTGTTT0
ATCGGCTTGGCCCTATGACAGTAGAATGAC0

DNA sequence

GGCGGCAACCCTGGCGCCCGTCATGCCACGGCGATGGGAATGCCGATCAGCCGAACGACGCTGTTGCATCGCGTTCGCGCCGGCGGAACCAAGCCGATCC
CTCCGGTCACTGTGCTGGGCGTGGACGATTGGGCTTGGCGTAAGGGGCATCGCTACGGCACGATCCTGTGCGATCTGGAGCGTCGGCGGGTCATCGACCT
GTTGCCCGACCGCAGTGCCGACACCTTGGCGGCATGGCTCATGATGCACCCCGGCATCTCCGTCGTCGCCCGTGACCGCGCCGGCGCCTACGCCGAGGGG
GCCGCCCGTGGTGCTCCCGAAGCGATCCAGGTCGCGGACCGGTGGCATTTGCTGCGAAACGCGAGCGACGCCCTGCGCGGGGTTCTCGAGCATCATCACC
GCGATCTGCGCGAGGCCGCTCAAACGGCCGCCCTGAAACCGGAACCGCTGACGCTGGAGGAGAACAACTCGGGACCAGCCACTGCATCCGAAACCGAACG
GCCGTTGCGAGCCGCAGAGCGGCGGTCGCGGGCTGCGCAGGAGCGCCGCGACGCCCGCTTCGCCGAAGTTGCGCGCTTGCGTGAACAGGGCATGGCGCTG
AAAGCCATTGCCCGCACCACCGGGATCGAACGCAAGACGGTGCGCCGCTGGTTGCGGGCCGGCCATGCACCGACATGGCGCCATGCCGCTCGCGCGACCA
GCATCCTCGACCCGTATCGCGCCCATCTGGAGGAGCGTTGGCAAGCCGGGTGTCACAATGGGGCAGCGCTCTGGCGCGACCTCAAGGGCCAGGGCTTTGC
CGGACAATATTCCGTTGTGCGGAACTGGGCGATGCAGCGGCGGCGAGAGGACCCGAGCCTCAGAAAATCCACAGGACCGAGGCGTCACTCGGCGGCGACC
AGAGTTACGGAGCCACCGACGCCTCGGCGGGCAGTGCGCCTGCTAACCGTCGACCTCGACGTGCTTTCCGAGGAGGACCGCCAGTTCGTCGTCTCCTTGC
GGGACCGCTCGCCCGCCATCGCCACCGCCGCCGACCTGATCAGCCGCTTCACGACGATGGTGAAGGACAAGACGCCGACCATGTTCGACGGCTGGCTGCG
TGAGGCGGAGGCCAGTGCCCTGGGGCCGTTCGCCGCCGGCATCCGGCGCGACGAGGACGCCGTGCGCGCCGCGCTCACCGAGCCGTGGAGTAGCGGGCAA
GTCGAGGGCCACGTCAATCGGCTCAAGGTCATCAAGCGGGATATGTACGGCCGTGCCGGCTTCGATCTCCTACGCAGTCGGGTCTTGGCTCACGCCTAAC
GCGAACATCACATCCCCAAACACCTTCTACTCATCCACACAAAATTTCCAAGAGCCCAAATTGGGATGTCGCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1266 bp412 aa341299+No
ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MGMPISRTTLLHRVRAGGTKPIPPVTVLGVDDWAWRKGHRYGTILCDLERRRVIDLLPDRSADTLAAWLMMHPGISVVARDRAGAYAEGAARGAPEAIQV
ADRWHLLRNASDALRGVLEHHHRDLREAAQTAALKPEPLTLEENNSGPATASETERPLRAAERRSRAAQERRDARFAEVARLREQGMALKAIARTTGIER
KTVRRWLRAGHAPTWRHAARATSILDPYRAHLEERWQAGCHNGAALWRDLKGQGFAGQYSVVRNWAMQRRREDPSLRKSTGPRRHSAATRVTEPPTPRRA
VRLLTVDLDVLSEEDRQFVVSLRDRSPAIATAADLISRFTTMVKDKTPTMFDGWLREAEASALGPFAAGIRRDEDAVRAALTEPWSSGQVEGHVNRLKVI
KRDMYGRAGFDLLRSRVLAHA

 

Blast result :
Comments
ISAzs8 is 62% aa similar to ISAli18.
References
1] ISfinder annotation (2010)
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.