ISAzs12
- Family ISAs1
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_013854 | ND | Azospirillum sp. | Azospirillum sp. B510 plasmid pAB510b Azospirillum sp. B510 plasmid pAB510f Azospirillum sp. B510 Azospirillum sp. B510 plasmid pAB510d Azospirillum sp. B510 plasmid pAB510c |
DNA section
IS Length : 1196 bp
Ends
IR Length : 14/15
IRL : CAGGGGAATCGCATACGGAAAAGCCTTGCCATTCCTATCGGATTTGATTC
IRR : CAGGGCAATCGCATATCAGATTTGGGCGAGCAGTTTTCGGAGGAAGGCGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACCGGGCATT | GGAACCGGGA | TCACTTCAGC | 10 |
| AGGCGGGAGG | ATCGCACGCC | TCCTTCTTCG | 10 |
| AGAGCGGAGG | GGGCCGGGCG | GCATCGCCCG | 10 |
| CTGGAATTCA | AGGCTTACCC | GATCATGACG | 10 |
| CCCAATCAGG | CAAGGCGGAC | GGAAGGCACC | 10 |
DNA sequence
CAGGGGAATCGCATACGGAAAAGCCTTGCCATTCCTATCGGATTTGATTCTACCCGGCGGACCGATTTGGGCGTTGGGGGAACGAGATGGAAGGGTTCAA
AGCGTGCTTTGGAGCGCTGGACGATCCACGACGGGGCAATGCTCGTCAACACGACCTTCATGAAATGCTGATGATCGCTCTCTGCACGTTCCTGTGCGGT
GGGCGAACCTGCATCGACATGGCGGAGTTCGCTGACGAACGTCAGGAGTTTCTGGGCGAATTCCTCACCTTAAAGGGCGGTCCGCCATCGCACGATACCT
TCAGCCGGCTGTTTCGTCTCCTTGATCCCAAGCAGTTTCAGGCCTGTTTCCGCACCTTCATGGACCGCTTTGCCGAGACCTGCCAGGGCGTCATCGCCAT
CGACGGCAAGGCGCTGCGGCGCTCCTTCGATACGGCGAGTGCTAAGTCGCCCCTGCACATGGTCAGCGCCTGGGGGTGCGAGCAGCGTCTGGTGCTCGCT
CAGATCGCCACCGACGCCAAGTCCAACGAAATCACCGCCGTGCCCACGTTGTTGGAGATGCTGGCGCTCAAAGGGACCATCGTGACCGTCGACGCGCTCA
ACTGCCAGCGCGACATCGCCCAGAAAATCATCGATCGGAAGGGCGACTACGCCTTGGCGCTCAAAGGCAATCAGGGCACGCTCCATGCTGACGTGAGCCT
GTTTCTCGACGATCCCGAGACCACGACCACCACCAGCCACACCACCGTTGATGGCGATCATGGCCGCATTGAGACCCGTACCAGCGTCGTCTCCACCGAC
ATTACCTGGCTCCAGGCCATTCACCAATGGCCTGGGCTGGCGGCCATCGGCAAGGTGGTCCGTACCCGCGAAACCCCGGCCAAGACGACGACGGAGACCG
CCTACTACCTGTTCAGCACGCCGCTCTCCGCCGAACGCTGCGGTGAGGTCGTCCGCGCCCATTGGGGCGTGGAGAACAGCCTGCATTGGCGCCTCGACGT
CACCATGAACGAGGATCAAGCCCGTAACCGCAAGGACAACGGCCCCGAAAACCTCGCCGTCCTCCGGCACCTCGCCATCAACCTCGTCTCAAAGGATGCT
TCCAAGGGGTCTACCCGCGTCAAGCTGCTGCGAGCCGCCTGGAATTCCGCCTTCCTCCGAAAACTGCTCGCCCAAATCTGATATGCGATTGCCCTG
AGCGTGCTTTGGAGCGCTGGACGATCCACGACGGGGCAATGCTCGTCAACACGACCTTCATGAAATGCTGATGATCGCTCTCTGCACGTTCCTGTGCGGT
GGGCGAACCTGCATCGACATGGCGGAGTTCGCTGACGAACGTCAGGAGTTTCTGGGCGAATTCCTCACCTTAAAGGGCGGTCCGCCATCGCACGATACCT
TCAGCCGGCTGTTTCGTCTCCTTGATCCCAAGCAGTTTCAGGCCTGTTTCCGCACCTTCATGGACCGCTTTGCCGAGACCTGCCAGGGCGTCATCGCCAT
CGACGGCAAGGCGCTGCGGCGCTCCTTCGATACGGCGAGTGCTAAGTCGCCCCTGCACATGGTCAGCGCCTGGGGGTGCGAGCAGCGTCTGGTGCTCGCT
CAGATCGCCACCGACGCCAAGTCCAACGAAATCACCGCCGTGCCCACGTTGTTGGAGATGCTGGCGCTCAAAGGGACCATCGTGACCGTCGACGCGCTCA
ACTGCCAGCGCGACATCGCCCAGAAAATCATCGATCGGAAGGGCGACTACGCCTTGGCGCTCAAAGGCAATCAGGGCACGCTCCATGCTGACGTGAGCCT
GTTTCTCGACGATCCCGAGACCACGACCACCACCAGCCACACCACCGTTGATGGCGATCATGGCCGCATTGAGACCCGTACCAGCGTCGTCTCCACCGAC
ATTACCTGGCTCCAGGCCATTCACCAATGGCCTGGGCTGGCGGCCATCGGCAAGGTGGTCCGTACCCGCGAAACCCCGGCCAAGACGACGACGGAGACCG
CCTACTACCTGTTCAGCACGCCGCTCTCCGCCGAACGCTGCGGTGAGGTCGTCCGCGCCCATTGGGGCGTGGAGAACAGCCTGCATTGGCGCCTCGACGT
CACCATGAACGAGGATCAAGCCCGTAACCGCAAGGACAACGGCCCCGAAAACCTCGCCGTCCTCCGGCACCTCGCCATCAACCTCGTCTCAAAGGATGCT
TCCAAGGGGTCTACCCGCGTCAAGCTGCTGCGAGCCGCCTGGAATTCCGCCTTCCTCCGAAAACTGCTCGCCCAAATCTGATATGCGATTGCCCTG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1095 bp | 364 aa | 87 | 1181 | + | No |
Chemistry : Unknow
ORF sequence :
MEGFKACFGALDDPRRGNARQHDLHEMLMIALCTFLCGGRTCIDMAEFADERQEFLGEFLTLKGGPPSHDTFSRLFRLLDPKQFQACFRTFMDRFAETCQ
GVIAIDGKALRRSFDTASAKSPLHMVSAWGCEQRLVLAQIATDAKSNEITAVPTLLEMLALKGTIVTVDALNCQRDIAQKIIDRKGDYALALKGNQGTLH
ADVSLFLDDPETTTTTSHTTVDGDHGRIETRTSVVSTDITWLQAIHQWPGLAAIGKVVRTRETPAKTTTETAYYLFSTPLSAERCGEVVRAHWGVENSLH
WRLDVTMNEDQARNRKDNGPENLAVLRHLAINLVSKDASKGSTRVKLLRAAWNSAFLRKLLAQI
GVIAIDGKALRRSFDTASAKSPLHMVSAWGCEQRLVLAQIATDAKSNEITAVPTLLEMLALKGTIVTVDALNCQRDIAQKIIDRKGDYALALKGNQGTLH
ADVSLFLDDPETTTTTSHTTVDGDHGRIETRTSVVSTDITWLQAIHQWPGLAAIGKVVRTRETPAKTTTETAYYLFSTPLSAERCGEVVRAHWGVENSLH
WRLDVTMNEDQARNRKDNGPENLAVLRHLAINLVSKDASKGSTRVKLLRAAWNSAFLRKLLAQI
Blast result :
Comments
ISAzs12 is 57% aa similar to ISOan1.
References
1] ISfinder annotation (2010).
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.