ISAzs13

  • Family ISL3
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_013854 ND Azospirillum sp.
Azospirillum sp. B510
DNA section
IS Length : 1769 bp

Ends


IR Length : 21/23

IRL : GGTCCATCCGCATTCTCGGTGCATCCCCGATGCGGATGGGAGGCGAAATG
IRR : GGCCCATCCGCATTTTCGGTGCAGGGCAGCAGCCTGGGCGTGGGCATTCA

Insertion site


Left flankDirect repeatRight flankDR Length
GTCAGCGCCGGATGAATACTAAACTTCATCCGGCCTTTAC0

DNA sequence

GGTCCATCCGCATTCTCGGTGCATCCCCGATGCGGATGGGAGGCGAAATGAGCACATAAAGAGAATGATTTTCCTTTCAGAGTGCCAGCACCCTTGTCCA
AATTCCTGCTTTCGTTGCTCCCGGCCGGCCTTGCCGTCGATCAGGTTTCCACCGAGGCGGATCGTGTCGCCGTAACCGCTCACGTGCGGACTGCAAAGGC
GAGCTGTCCGCTGTGCCGTCGCCCCTCCGTGCGCGTCCACAGCCGGTACTTCCGACATCTGGGCGACCTGCCCTGGCAGGGCCGGATCGGCGAACTCCGT
CTGCAGGTCCGTCGCTTCCGCTGTGCGACGGCGGACTGTTCCCGCCGTATCTTCGCCGAACGCCTGCCCACCGTGGCGGCGCCTCGGGTCAGGCGCACCC
GGCGGCTTGCCGAGGCGCAGCGCACCATTGCGTTGAGCGCGGGCGGGGATCCCGGCGCCCGGCTGTCCACCCGGCTCGCCATGCCGGTCAGCGGCGACAC
GCTGCTGCGTTTGATCCGGGCGGAGCCGGTGCCGCCGGTCTGCGAAGCCCGCATCATCGGAATCGATGATTGGGCTTGGCGCCGGGGCAAGCGCTACGGC
ACCATCGTCGTCGATCTCGAGCGAAATCATCCCATCGACCTTCTGCCAGACCGCCAAGCCGACACCGTTGCCACATGGCTGAAAGCCCGTCCTGGCGTCG
AGATCGTCGCCCGTGACCGTGCCGGTGCTTACGCCGATGGCGTCCGCCAGGGTGCTCCCGCCGCCGTCCAGGTCGCCGACCGCTGGCATCTTCTGCACAA
TCTCACCGATGCGCTGCGCGAGGTCCTCACCGGCCATCACCGCGACCTTCGGGCCGCCGCCAGGCTGGCTGTCTCTCCCGTGGGAGAAGCCGGGCCAGTA
GGAGAAACCGTGCAGGAGCCGCGGTCGCCGCGGTCGGATGAGGAGTCCAGTCCACCAACCCGGCGGGAGCTGCGGAGCGCTGCCATTCATGCCGCTCGCC
ATGCCCGCTACGAAGAAGTCGTCGCGCTGAAAGCCCGCGGGTGGTCGCAAACGCGCATCGCTGAAACGCTCCACCTCGACCGCAAGACGGTGCGGGTGTG
GCTGCGATCCGGGCAGCCGCCGGCATGGCGCCAGCCGGCCAAGGGGAGCCGGCTTGATCCGTTCCTCGATCACCTGCAGCAACGCTGGGATGACGGCTGC
CACAACGCCGCTCAGCTCTGGCGGGAGATCAGGTCCCGCGGCTTCACCGGTCAGCGCAGCATAGTCCGGGACTGGGCAAGGCTTCTGCGACAGGCCGTAC
GAAGCGCCGCGTCCATGGCATCGACATCATGGCCGGTGCCGTCGCGCCGCCGGGCAGCATGGTTGGTAATCGCCGACGAGCAGGAGATCGATCCGACCGA
ACAGGCCTTCGTCTCGGCCCTGATCGCCCGATCACCAGAACTAGGACGAACCATCACCGTGACCCGTGCCTTTCGGGCGATGGTCCGAGGACAACGAGCC
GGAGAACTGGACAACTGGCTGCTGACGGCGGAGGGCACCCCCTTGGCGGGCTTTGCCAATGGCTTGAAGCGCGATCTTGCTGCGGTCCGGGCGGCTCTGT
CCCTGCCGTGGAGCACTGGGCCAGTGGAGGGGCAGATCAGCCGGCTGAAGACGATCAAGCGCATGATGAACGGCCGCGGCGGTTTCGACTTGCTGCGCCA
TCGCATGCTCGAGGCCGCCTGAATGCCCACGCCCAGGCTGCTGCCCTGCACCGAAAATGCGGATGGGCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1641 bp546 aa821722+No
ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MPAPLSKFLLSLLPAGLAVDQVSTEADRVAVTAHVRTAKASCPLCRRPSVRVHSRYFRHLGDLPWQGRIGELRLQVRRFRCATADCSRRIFAERLPTVAA
PRVRRTRRLAEAQRTIALSAGGDPGARLSTRLAMPVSGDTLLRLIRAEPVPPVCEARIIGIDDWAWRRGKRYGTIVVDLERNHPIDLLPDRQADTVATWL
KARPGVEIVARDRAGAYADGVRQGAPAAVQVADRWHLLHNLTDALREVLTGHHRDLRAAARLAVSPVGEAGPVGETVQEPRSPRSDEESSPPTRRELRSA
AIHAARHARYEEVVALKARGWSQTRIAETLHLDRKTVRVWLRSGQPPAWRQPAKGSRLDPFLDHLQQRWDDGCHNAAQLWREIRSRGFTGQRSIVRDWAR
LLRQAVRSAASMASTSWPVPSRRRAAWLVIADEQEIDPTEQAFVSALIARSPELGRTITVTRAFRAMVRGQRAGELDNWLLTAEGTPLAGFANGLKRDLA
AVRAALSLPWSTGPVEGQISRLKTIKRMMNGRGGFDLLRHRMLEAA

 

Blast result :
Comments
ISAzs13 is 91% aa similar to ISAli11.
References
1] ISfinder annotation (2010).
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.