ISAsp2

  • Family ISAs1
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Anabaena sp.
Anabaena sp. 90
DNA section
IS Length : 1608 bp

Ends


IR Length : 30/34

IRL : CAGGGGAAACGCATCTAATTTTTTTTGACAAAATGTAACTTCTATGAATA
IRR : CAGGGGAAACGCATCTAAATTATCTTGACAGAATACTATATTAGTGCATC

Insertion site


Left flankDirect repeatRight flankDR Length
CATGGGGTTAGCTGATCAGGTAATTACAGT10
AGCAGTTCGCCCTATACGAC0
CCTCCATAGGAATATTCACC0
GATGGGCAAGTGTGGGTTATGACTACTTTA10
ATTTCACTTACTCCCTATTAGGGATTGAAA10
GACAGAATGTCTCCCTATTA0

DNA sequence

CAGGGGAAACGCATCTAATTTTTTTTGACAAAATGTAACTTCTATGAATAGATAAAAAGGGCAGTATTACCATGTTAATATCAAGCCAATAAATGAGTAT
GAAGAAAATTTGATGGTAAATTCGTTTCTATAAGAATCAATAAAATGAGTTGAAGTGGTATTCTCAATAAAACTAAAGAGACAAAATTCACAAAATGAAG
CTACCACCAAAAATCACAATAGTAGATCACTTTAAAGATTTAGAAGATAAAAGAGTTGAGAGAACAAAAAGGCATAAATTAATAGATATAGTAACCATTG
CGATTTGTGCAGTGATCTGTGGAGTAGATAGTTGGGTATTGATGGAGGCTTATGGAAAAAAGAAAGAAAAATGGCTAAAACAATTTTTAGAACTTCCAAA
CGGGATTCCATCTCATGATACATTCGCAAGAGTATTTGCGAGAATAGATCCGCAACAATTTCAGAATTGTTTTTTGAGTTGGATAAAATCTATCAATAAA
ATTACAGAAGGAGAAGTCATAGCAATAGATGGGAAAACATTAAGGCATTCATATGATAAAGGAAAGGATAAAGGTGCGATTCAGATGGTAAGTGCATGGG
CAACTAGTAATAAATTAGTATTAGGACAATGTAAAGTAGAAGAAAAGTCAAATGAAATAACAGCCATACCGGAATTAATTAAAGTATTAGATATAGCCGG
ATGTTTAGTAACGATTGATGCGATGGGGTGTCAAAAAGAGATAGTAAAATCAATTGCAGAAAAATCAGGCGAATATATTATCGCACTCAAAAAGAATCAA
GGTAATTTATATAAGAATGTAGAAGAAATCTTCAAAGAAGCTATATCTAAAGGGTTTGAGGGATTCAAATATAGTGAATTTCATACAAAAGAAGACAAAC
ATGGAAGAGAAGAGATTCGTCATTATCTCATGTTATCAGACATAGAAGAAAGAATAGATACTGATAAGAAATGGGTAAATCTTCAAAGTGTAGGAATGGT
AGAATATATACGAAAAGTTAATGGAAAAACGAAGGTTGAGACAGGCTATTATATAAGTAGTTTGACAAATAATGCGAAATTACTAGGAGAATCAGTCCGC
ACTCATTGGGGTATAGAGAATTCATTACACTGGGTTTTAGATGTAGCTTTTAGAGAAGATGATTGTCGGATAAGAAAGGATAATGCACCACAAAACTTTG
CAGTTATTCGTCATATAGCAGTTAATCTTTTAGGAAAAGAAAAAAGCCAAAAACTAGGAACTAAAAGTAAGCAGTTTTGTGCAGGATGGGATGATGAATA
TTTAGAGAAGATTTTAGAATGTATCTGATAAAAATCAGAAAATATAGACAAATATAAATATAACCAATTTATTTTTCTGTTGATTCTACAATAGAATCTA
TATTTTTGATGCCAAAAAATATAGATATTAATATATAGAACTGAACAAAATAAATCCATCATTAAAAATAAATAATTACTCTAAATTTATGAAATTTTTA
TAATTTTAATAAAGGAATTTATCAGATTTTGAATGAAGTTAGATATTTTCCCATATTGATGCACTAATATAGTATTCTGTCAAGATAATTTAGATGCGTT
TCCCCTG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1134 bp378 aa1951328+No
ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MKLPPKITIVDHFKDLEDKRVERTKRHKLIDIVTIAICAVICGVDSWVLMEAYGKKKEKWLKQFLELPNGIPSHDTFARVFARIDPQQFQNCFLSWIKSI
NKITEGEVIAIDGKTLRHSYDKGKDKGAIQMVSAWATSNKLVLGQCKVEEKSNEITAIPELIKVLDIAGCLVTIDAMGCQKEIVKSIAEKSGEYIIALKK
NQGNLYKNVEEIFKEAISKGFEGFKYSEFHTKEDKHGREEIRHYLMLSDIEERIDTDKKWVNLQSVGMVEYIRKVNGKTKVETGYYISSLTNNAKLLGES
VRTHWGIENSLHWVLDVAFREDDCRIRKDNAPQNFAVIRHIAVNLLGKEKSQKLGTKSKQFCAGWDDEYLEKILECI

 

Blast result :
Comments
ISAsp2 is 76% aa similar to ISCysp7. There are 6 copies on the genome.
References
1] Hao Wang (2009) Direct submission