ISAsp3

  • Family IS4
  • Group ISPepr1
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Anabaena sp.
Anabaena sp. 90
DNA section
IS Length : 1452 bp

Ends


IR Length : 21/24

IRL : CAATACTGTAGCCAAAAAAAGAGGATGAAGAGAGAGGGCTGATGTAGTAG
IRR : CAATACTGTAGCCAAATTACGAGGGTTCAACGGCTGTAGGAGCATTGTGA

Insertion site


Left flankDirect repeatRight flankDR Length
CGGGAATATCACCAATGGAA0
AAACCCTAATACTATACTTGTTTTA5
TTGCAATTTCACTTCTAAAATGAC4
AATTTCACTTACTATTACTA0
AATATATTCAGTTAACTAACACCCA5

DNA sequence

CAATACTGTAGCCAAAAAAAGAGGATGAAGAGAGAGGGCTGATGTAGTAGAGTTATAGTCTTCCAAAACAAAAACTCGAAAACCACATACAGCCCATGTT
TGATATCCTAACACTCCTGCAATGCCTGCTACCAGAAATAAAAGTGACGACTATGCGGCAATTGAGTCAGATCATCATGGCCATGTTAGCAATGAGCGGA
CGAGTAACGATGTTGGGAATTTCGCGTTGGACGCTTAGTGGTGGTAGTTATCGGACGGTAATCAGATTCTTTCGGACAGTCATACCGTGGGCAACGGTGT
TCTGGGTGTTTTTTCGGCGGCATTTGTTTTGCCCAAATGATGTTTATTTGTTGGCAGGAGATGAAGTAGTAATAAGCAAAGCCGGGAAAAAAACCTATGG
ATTAGATAGGTTCTTCTCAAGTCTAACAAGCAAACCAATATCGGGGCTATCTTTCTTTACCTTATCATTAGTAAGTGTTCAACAAAGACACTCATTTCCG
ATTCAGATAGAACAGGTAATCAAGAGCGATGTAGAAAAAAGTATTGTCTCACCAATTCCAGAAGTAAAACCTCAAGAAAAACCTGGACGGGGACGACCAA
AAGGGAGTAAGAACAAAAATAAACAGGAGGTGATTTTTACATCTGAACTACTAAGAATTCAGAAAATGATTAATGAGCTATTTAAGTTAATAGCTAACTT
TATCCCCCTCACTTACTTGGTTGTAGATGGTCACTTTGGGAACAATAATGCTTTGCAAATGGCTAGACAGGTAAAGTTACACATAATTTCCAAATTACGC
CACGATTCGGCGTTATACATACCTTACCAACATCCTGACCCTAATCATCGTTCTCGTCGTAAATACGGAGATAAGCTGGACTGGCGTAATATTGCTGGGG
AATATTTACGTCAAAGCAGTATTGACGAAGATATCAAAACTGATATTTATCAAATGACTCTACTGCACAAAGAATTTGCTCAATCCTTGAATGTAGTGAT
TTTGGTAAAAACAAATATCAAGACGAATGCTGTTAGTCATGTGATTTTATTTTCCAGTGACTTAGATTTGTCTTATGAGAAAATAATCGACTACTATAGA
CTCCGCTTTCAAATCGAATTTAATTTTCGTGATGCCAAGCAGTTCTGGGGATTGGAAGATTTTATGAATCGGGGTCAAACTGCGGTGACTAATGCGGCCA
ATCTTTCTTTTTTTATGGTCAACTTATCTCATTATCTTTTAGCTCAATTCCGTCAAGATAATCCTGGTGCTGGAATTATTGATCTTAAGGCTTACTGTCG
TGGTTTTCGATATGTTCGTGAAATGTTAAAAATGCTTCCGGAACAACCAGAGCCTATTTTATTAGCCCAGATTTTTGCCAAGCTTACCTCTCTTGGTCGT
GTTCACAATGCTCCTACAGCCGTTGAACCCTCGTAATTTGGCTACAGTATTG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1341 bp446 aa961436+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MFDILTLLQCLLPEIKVTTMRQLSQIIMAMLAMSGRVTMLGISRWTLSGGSYRTVIRFFRTVIPWATVFWVFFRRHLFCPNDVYLLAGDEVVISKAGKKT
YGLDRFFSSLTSKPISGLSFFTLSLVSVQQRHSFPIQIEQVIKSDVEKSIVSPIPEVKPQEKPGRGRPKGSKNKNKQEVIFTSELLRIQKMINELFKLIA
NFIPLTYLVVDGHFGNNNALQMARQVKLHIISKLRHDSALYIPYQHPDPNHRSRRKYGDKLDWRNIAGEYLRQSSIDEDIKTDIYQMTLLHKEFAQSLNV
VILVKTNIKTNAVSHVILFSSDLDLSYEKIIDYYRLRFQIEFNFRDAKQFWGLEDFMNRGQTAVTNAANLSFFMVNLSHYLLAQFRQDNPGAGIIDLKAY
CRGFRYVREMLKMLPEQPEPILLAQIFAKLTSLGRVHNAPTAVEPS

 

Blast result :
Comments
ISPepr1 is 41% aa similiar to ISDra7. There are 4 complete copies and 1 frameshifted copy on the genome.
References
1] Hao Wang (2009) Direct submission