ISAba16

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
JN415682 ND Acinetobacter baumannii
Acinetobacter baumannii
DNA section
IS Length : 2552 bp

Ends


IR Length : 12/16

IRL : GTAAGCATCCGGCTAACACAGCCTTACCAAGCGATCCTATAAGCCTTAAT
IRR : GTAAGCGTCCGCTGAATCCCATACCAATTTTAATTCGATTTAGGTTTCCA

Insertion site


Left flankDirect repeatRight flankDR Length
CATTAAAACACTCTTACTGTAAGCATCC8
GGACGCTTACCTCTTACTTATAACAAGC8

DNA sequence

GTAAGCATCCGGCTAACACAGCCTTACCAAGCGATCCTATAAGCCTTAATTTAGTTCCCACCTAAAAACAAGTGGGGACTTAATCCATGAACATGGATAT
ATATTCAGCAACACCTCCTGTTGCTAAAAAACGCAGAACATACAGCAAAGAATTCAAACTCAGTATTGTCAATGCCTGTAAAAATCCTAATACCTCGATC
GCTTCGGTCGCACTGCAACATAGTATTAATGCCAACCTTGTCAGTCGTTGGATCAGGATCTTCAGCCATCATGAGGGTGCCGTGCAGGATCCTACTCATG
TGAATCCAGCATTTATTGCTTTGCCTTACACTGCTGCAATCAGCCAACCTATTGATGAGAGGATCACGTTGTGTATCACCGTGCCTCATACGAATAATGA
TATTCAGCTAAAATGGCAGACATCAGAAATACCTGCCTTGGCAGAATTACTCAAGGCACTTGCAACATGATCCGCATTGATGAAATCTGGTTGTCTACTC
AGCCCATGGACATGCGTGCAGGTATGGATACGACCATGGCTCAGGTGGTGAGAGCCTTTGGCTACATCAAACCGCATTGTGCTTACCTGTTCTGTAATAA
ACGTGGCCATCGCATGAAAGTACTGGTACATGATGGACTGGGCATCTGGCTGTGTGCCCGGCGGCTGGAACAGGGCAAATTTCACTGGGCTCAAGTTCAC
CAAGGTGAAACCGTGGCCCTCAGCCCGGAACAGTTACAGGCACTGATCCAAGGTTTGCCCTGGCAGCGCATTGGACGACAGCAGGTGGTGACGATGCTTT
AAACCAGGCTGTTCCATTCTGCTATTCTCCAAACGTTCTATTTCATTCTTCTCATGACCTCAGGCATACTGCGGTCATGAATACGCTGCCTGACTTAAGC
CAACTGACCCATGAACAACTGCTGGAATTCACCAGACAGTTGGCGATGCAGCATCAGTCTCTAGCACAATCAAATCAAGAATTAGAAAAATCAAACCAGC
AATTGGATGCCAAAGTTCAACATCTTGAAGTCACCAATCAGCAATTAGATTCTAAAGTTCAACATCTTTCTATTCTCAATCAAAAATACGAGCATGAACT
CGCACTATTTAAACAGCACAAATTCGGCAGTAAAAACGAACATCTCACTGCAAAACAAATCCACCTGTGGGATGAAGCGGTTGAAGAAGATATTGCTGCG
GTTGATCTAGAACTGGAACGGCTAAATGCAGATAAAACCGATGCAGCGACACAGAAAGCCAAAACCAATAAACCTAAACGTCGACCACTGCCAGATCATC
TACACACCATCCGTATTGAGCATGAACCTGCATCAACCCAATGCGCTTGTGGCTGCCAACTCCGTCGTATCGGCGAAGATGTCAGTGAAAAACTGCATTT
CAGACCGGCACAGTTCTATAAGGAACAGCATGTGCGTGGTAAATGGGTCTGTGATCAGTGTGACACCCTGACTCAGCAAGCGATGCCCGCCTATGTGATT
GATAAAGGCATTGCTTCACCTGAACTGCTCAGCCATGTTTTGGTATCGAAATATGCCGATCATTTGCCGCTGTACCGTCAACGTCTGATCTATCAGCGGG
CGGGAATCGAACTTTCTAGATCAACTTTATCTGACTGGATAGGTCGCTGCGGTGTAGAACTGGAACCTCTAGCCAATGCCTTAAAAGAGGTGGTACTACA
ACAGCAGGTGCTGCATGCAGATGAAACACCGGTCACCATCATGCGGATGGGTGAGAATGATAAAAAACCGAAGAAAGGTTATGTCTGGGCCTATGCCACT
ACACAGTACAATCCAGTTCAGGCGGTGATCTATGACTTTCAGGATAGTCGTTCAGGCCAGCATGCTGCAGAGTTCTTGAAAGGCTGGCAGGGTTATCTAG
TCTGTGATGATTACAGTGGTTATAAAGCACGCTTTAAATCAGGCCAGGTCATTGAGGTGGGCTGCATGGCCCATGCACGTCGTAAATTCCATGAGCTACA
TGTGACTGGGAAAAGTCAGATCGCTGAACAGGCATTACTGATGATTCAGAAACTGTATGCGATAGAAGCAGAACTCAGGAAAAAGACCGATAGTACAGCA
GAACACCGCCGCGAATACCGACAACAGCATAGTCAACCAGTGATGCAACAACTATATGAATGGCTCAACCAACATCATCTGACAGTGCCATCGAGTTCTC
CCACCGCCAAGGCCATCAATTACACTCTGAAGCGTTGGCCAGCTTTAAGCCGCTATCTGGATGATGGCAATCTACCTATTTGCAATAATTGGGTCGAGAA
TCAGATGCGTCCCTGGGCGTTGGGGCGCAAGAACTGGCTGTTTGCAGGTTCGCTGCGCAGTGGTCAGCGAGCGGCAAACATCATGACGTTAATCCAGTCA
GCAAAGCTGAATGGCTTGGATCCGTATGCCTATTTAAGTGATGTGCTGAAAAGGCTGCCGACACATAAAGTGACCCAGATTGAAGAGTTACTGCCACACT
GCTGGAAACCTAAATCGAATTAAAATTGGTATGGGATTCAGCGGACGCTTAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
384 bp127 aa87470+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MNMDIYSATPPVAKKRRTYSKEFKLSIVNACKNPNTSIASVALQHSINANLVSRWIRIFSHHEGAVQDPTHVNPAFIALPYTAAISQPIDERITLCITVP
HTNNDIQLKWQTSEIPALAELLKALAT

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
336 bp111 aa467802+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MIRIDEIWLSTQPMDMRAGMDTTMAQVVRAFGYIKPHCAYLFCNKRGHRMKVLVHDGLGIWLCARRLEQGKFHWAQVHQGETVALSPEQLQALIQGLPWQ
RIGRQQVVTML

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1647 bp548 aa8772523+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNTLPDLSQLTHEQLLEFTRQLAMQHQSLAQSNQELEKSNQQLDAKVQHLEVTNQQLDSKVQHLSILNQKYEHELALFKQHKFGSKNEHLTAKQIHLWDE
AVEEDIAAVDLELERLNADKTDAATQKAKTNKPKRRPLPDHLHTIRIEHEPASTQCACGCQLRRIGEDVSEKLHFRPAQFYKEQHVRGKWVCDQCDTLTQ
QAMPAYVIDKGIASPELLSHVLVSKYADHLPLYRQRLIYQRAGIELSRSTLSDWIGRCGVELEPLANALKEVVLQQQVLHADETPVTIMRMGENDKKPKK
GYVWAYATTQYNPVQAVIYDFQDSRSGQHAAEFLKGWQGYLVCDDYSGYKARFKSGQVIEVGCMAHARRKFHELHVTGKSQIAEQALLMIQKLYAIEAEL
RKKTDSTAEHRREYRQQHSQPVMQQLYEWLNQHHLTVPSSSPTAKAINYTLKRWPALSRYLDDGNLPICNNWVENQMRPWALGRKNWLFAGSLRSGQRAA
NIMTLIQSAKLNGLDPYAYLSDVLKRLPTHKVTQIEELLPHCWKPKSN

 

Blast result :
Comments
ISAba16 is 65% (orfA), 98% (orfB) and 96% (orfC) aa similar to ISAba17.
References
1] Lopes et al.(2011). Journal of Antimicrobial Chemotherapy. Accepted for publication.