ISAcsp3

  • Family IS3
  • Group IS3
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Acinetobacter sp.
Acinetobacter sp.
DNA section
IS Length : 1237 bp

Ends


IR Length : 30/39

IRL : TGATCCTAGCCAAAATAATGGACAGCACTTCCCTCTAAAGATAACGTAAC
IRR : TGATCCTAGACAGTATTGTGGACAACCGATCCCTCTAAATTCTTAAAATA

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

TGATCCTAGCCAAAATAATGGACAGCACTTCCCTCTAAAGATAACGTAACGTTGACTTTGGAGATACAAGAAATGGCCAAACGTTTTAGTCCTGAATTTA
AACAGCAAGCAATTGATTATGCACTTTCAAACTCACACGAGTCTGTAGCTGCAGTCGCCCAGAAATTAGGTGTGGGTTATTCAACATTAGATAAATGGAT
TCGTGAAACCAATCCAGCAGGTTCAAGCAAACGTCAACTTTCACCAGAACAACAGCGGATCTTGGAATTAGAGAAAGAAGTCAAACAGCTCAGGGAAGCC
AATGACATCTTAAAAAAAGCGCATGTGTACTTTCTGACAGATCATGCCAAGAAAAGTACACGGTAATTCAAGATCTAGATATGAGCGAAGTCACTATCTC
TTCTGCCTGTAAATGCCTGGGTGTCAGCACTTCAGGCTATTATGCCTGGCGAAAACGCCAGGCCAATACAGCGCAGAAATATAATGATTTAAAAGCCGTA
TATTGGCAGCATCATGCACGATTGGGTGCACCTTCATTGGTACATGACATGCATGATTTAGGTTACAGCATGAGTGAACGCACTGTTGGAAGAATGCTGA
AAAAGCTGGGTTTACGCAGCAGGATTGCGCGTAAATACAAGCATACGACTGATTCAAACCATCGTTTGCCTACAGCACCCAATCTGTTAGAGCGTCAATT
TACAGTCATTCAGCCTAACAAGGTTTGGACAACGGATATTACCTATATCCGTACCAAGCAAGGTTGGCTATATTTATGTGTGATGCTGGATCTATTTAGT
CGTCGTATTGTGGGGTGGCAAACCAGCCATCGAATAGACCGTCAATTGGTGTGTGATGCGTTTCATTATGCAATGGCTCGTCAGGGGTATCCAATGGGTG
TCATGGTACATTCGGATCAAGGCTCACAGTACTGTAGTCGTGATTTTAGAACGCTATTATTAACGAATAACTGTATTCAAAGCATGTCACGCAGAGGGAA
CTGTTGGGATAATGCAGTGACCGAAAGCTTTTTTCATACATTGAAAGGTCATGTGGTACATGACAGTGTGTTTGCCACCCGAAAAGAGGCGAATGCTGTC
TTGTTTGACTATATTGAGATTTATTACAATCGGGTCAGAAGGCATTCTGCAAATGGTTGGTTAAGCCAAGAAGCTTTTGAACAGAAATATTTTAAGAATT
TAGAGGGATCGGTTGTCCACAATACTGTCTAGGATCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
294 bp97 aa73366+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MAKRFSPEFKQQAIDYALSNSHESVAAVAQKLGVGYSTLDKWIRETNPAGSSKRQLSPEQQRILELEKEVKQLREANDILKKAHVYFLTDHAKKSTR

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
927 bp308 aa3061232+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

HLKKSACVLSDRSCQEKYTVIQDLDMSEVTISSACKCLGVSTSGYYAWRKRQANTAQKYNDLKAVYWQHHARLGAPSLVHDMHDLGYSMSERTVGRMLKK
LGLRSRIARKYKHTTDSNHRLPTAPNLLERQFTVIQPNKVWTTDITYIRTKQGWLYLCVMLDLFSRRIVGWQTSHRIDRQLVCDAFHYAMARQGYPMGVM
VHSDQGSQYCSRDFRTLLLTNNCIQSMSRRGNCWDNAVTESFFHTLKGHVVHDSVFATRKEANAVLFDYIEIYYNRVRRHSANGWLSQEAFEQKYFKNLE
GSVVHNTV

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1160 bp386 aa731232+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAKRFSPEFKQQAIDYALSNSHESVAAVAQKLGVGYSTLDKWIRETNPAGSSKRQLSPEQQRILELEKEVKQLREANDILKKSACVLSDRSCQEKYTVIQ
DLDMSEVTISSACKCLGVSTSGYYAWRKRQANTAQKYNDLKAVYWQHHARLGAPSLVHDMHDLGYSMSERTVGRMLKKLGLRSRIARKYKHTTDSNHRLP
TAPNLLERQFTVIQPNKVWTTDITYIRTKQGWLYLCVMLDLFSRRIVGWQTSHRIDRQLVCDAFHYAMARQGYPMGVMVHSDQGSQYCSRDFRTLLLTNN
CIQSMSRRGNCWDNAVTESFFHTLKGHVVHDSVFATRKEANAVLFDYIEIYYNRVRRHSANGWLSQEAFEQKYFKNLEGSVVHNTV

 

Blast result :
Comments
ISAcsp3 is 97% aa similar to IS1236.
References
1] Lopes, B. S. (2015) Direct submission