ISAeme12

  • Family IS4
  • Group IS4
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP007567 ND Aeromonas media
Aeromonas media WS
DNA section
IS Length : 1426 bp

Ends


IR Length : 16/18

IRL : TAATGCCGATCAGTTAAGGATCCATTGACCGATCCTCCTGATGTGTAAAA
IRR : TAATGCCAGTCAGTTAAGCCTGACTGGCATTGTTTTTATTGGCTTTTTTC

Insertion site


Left flankDirect repeatRight flankDR Length
TGAAAAACCCGCCACCCTGGCGGGTTTTTCTC12
AAAAAGAGAGCGCCCTTGGGCGCTCTCTTGCA12

DNA sequence

TAATGCCGATCAGTTAAGGATCCATTGACCGATCCTCCTGATGTGTAAAAATCCGAAACCTGTTGATTTGGTTTCGGATTTTTTATGCGTATCGAACAGG
CTCTCGACTTCCTCCACGCCAGCAATATGGCCCAGTTTGAGTCACTCTCTGACCTCATTCCCCCCGCGCTCATCACCTCCCTGCTCGGCGAAGAGGGCGT
CGCCACTCTGCGCCGTCGCCGCATGCCCATGGAGCGCCTGGTCTGGGCCATCATCGGCATGGCCATCTTCCGCAACGTCCCCATGACCCAACTGGTCAAT
CAGCTCGATATCCTGCTGCCCGGCGACCGCCCCTTCGTCGCCCCCAGCGCCTTCCTGCAAGCCCGCCAAAAGCTTGGCCACAAGAGCATCGCGCGCCTGT
TCCACGACACTGCCACCCGCTGGCATCAACAGGCCAACCACCCCGGCTGGGCCGGACTGCAACTGCTCGCCGTCGATGGCGTGATGTGGCGCACACCCGA
TACACCGGACAATGCCGCCGCCTTTGCCAAGCCCGGCACCCAGCATGGCGAGACCGCCTATCCCCAGGTGCGCATGCTCTGTCAGATGGAGCTGACCAGC
CATCTGCTGGTTCAGGCCGTGATGGAGAGTTGTGCCGTCAACGAGATGGTGCTGGCCGAGCAACTTATTGCACGCACACCGGACCACTCGCTGACCCTGT
TCGACAAAGGCTTTTACTCCCTTGGCCTGCTCCACGCCTGGCAGCGTGCTGGCAGTGAGCGGCACTGGCTGCTGCCGCTCAAGAAGGGCACCCAATACGA
GGTGGTGCGCAAGCTGGGGCGCCAGGATGCGCTGGTGCAGCTCAAGACCAGTCCGCAGGCGAGGAAGAAGTGGCCGCAGTTGCCAGAGGCAATAGAAGCC
CGCTTGCTGACGCGCACTATCAACGGCAAGGAGCGGCAGGTGCTGACCTCGATGGTCGACCCGATGCGCTTCCCGGGGGCTGACATCGTGGAGCTGTATG
GCCATCGCTGGGAAATCGAACTGGGGTATCGGGAGATGAAACACAGCCTGCAGCAGCACAGACTGACCCTGCGCAGCAAGAAGGCGACGGGGATACGGCA
AGAGCTGTGGGGCGTGCTGCTGGCCTATAACCTGCTGCGTAGCCAGATGGTGAAGATGGCGGCGAGCCTGAAGGGATACACGGCGAGCCAGCTGAGTTTT
CACATGGCGTCGGTGTACCTCATCCATGAACTGAGCTGCATGCCGTATGTGTCACCGGGGAATATCCCGGGGCGGGTGGCGGAGCTGGAGAAGCAGGCGG
GGCAGTTTGTGCTGCCGGCACGAAGAGAGCGAAGTTACCCTCGCTGCGTGAAGCCAAGACCACAGAAGTACAGTGTGAAAAAAGCCAATAAAAACAATGC
CAGTCAGGCTTAACTGACTGGCATTA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1329 bp442 aa851413+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRIEQALDFLHASNMAQFESLSDLIPPALITSLLGEEGVATLRRRRMPMERLVWAIIGMAIFRNVPMTQLVNQLDILLPGDRPFVAPSAFLQARQKLGHK
SIARLFHDTATRWHQQANHPGWAGLQLLAVDGVMWRTPDTPDNAAAFAKPGTQHGETAYPQVRMLCQMELTSHLLVQAVMESCAVNEMVLAEQLIARTPD
HSLTLFDKGFYSLGLLHAWQRAGSERHWLLPLKKGTQYEVVRKLGRQDALVQLKTSPQARKKWPQLPEAIEARLLTRTINGKERQVLTSMVDPMRFPGAD
IVELYGHRWEIELGYREMKHSLQQHRLTLRSKKATGIRQELWGVLLAYNLLRSQMVKMAASLKGYTASQLSFHMASVYLIHELSCMPYVSPGNIPGRVAE
LEKQAGQFVLPARRERSYPRCVKPRPQKYSVKKANKNNASQA

 

Blast result :
Comments
ISAeme12 is 96% aa similar to ISAs30.
IS_pep_1 is B224_1280.
References
1] Pfeiffer F. (2015) Direct submission
2] Chai B., Wang H. and Chen X. (2012) J. Bacteriol. 194 (23), 6693-6694