ISAeme20

  • Family IS3
  • Group IS3
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP007567 ND Aeromonas media
Aeromonas media WS
DNA section
IS Length : 1264 bp

Ends


IR Length : 23/31

IRL : TGAGGTATCCTGATTTTCACAGACAGTCCCGATTGTTACGATGAGACAAT
IRR : TGAGGTGTCCTGACAAGATCGGACAGTCCCGGATCAAGCAGCCTGAGCAG

Insertion site


Left flankDirect repeatRight flankDR Length
AGTGAAGGCGAGTATCGCTT0
ACAACCACCCAACAGACGCA0

DNA sequence

TGAGGTATCCTGATTTTCACAGACAGTCCCGATTGTTACGATGAGACAATCGGGGGTATTGAATGAAATCGAGAGAACACTATTCACCAGAGTTTCGGGC
AGAGGCGGTTCGACTGGTGTTGGAGCAAGGCCTTGGCCAAGCTGAAGCCGCCAGAAGGTTGGGTATTGCCAAAGGCACACTGAGCAACTGGGTGGTCGCA
GCCAAAGGAGGAAAGGATACATCGGCCATTCCCGGAGCGCGTTCTGTCGCCGAGCTGGAGGCTGAAGTAGCCCGGCTACGCAAAGCTCTCGCACAAGCCA
ACATGGAGCGCGACATCATAAAAAAGGCCGCCGCGTACTTTGCTCAGGAGTCACTGCCAAGTACGCGTGGATAGCCAACCAACAGGACATCTATCCCATT
TCCGTTATCTGTCGTGTGCTGGAGGTCTCTCGTGCGGGCTTCTACCACTGGCGCCAGCGTCCACCGTCACAGCGCCAACAAGAAGATGAACGACTCAAGG
TCGCCATTCGGGCTGCTCACAGCAAGACGCGCCAGACCTATGGCGCATCGCGTTTACAAGCTGAGTTGGCCGCCGAAGGATTTTACGCTGGGCGGGACCG
GATTGGGCGATTGCGCCGTGAAATGGGGTTGTCTTGCAAACAAAAGCGCAAATTCAAGGCGACAACCCATTCTGCTCATTCCCTGCCCGTGGCGGAGAAT
CTCCTTGGTCAGGTCTTTGCGCCGACGAAGCCCAACGAAGTCTGGACCGGTGATATCACCTATATCGCCACCGACGAAGGCTGGCTTTATCTGGCTGGTC
TCAAGGATGTGTTTACCTGTGAGATAGTGGGTTATGCGATGGGAGAGCGAATGACCACCGGGTTGGTCAGTCAGGCGCTGTTTCGGGCTGTGCAGCAGAA
GAGGCCACCCGTTGGGCTTATTCATCACACAGACCGGGGCAGTCAGTACTGTGCCAAGGCTTACCGGGCTCTCCAGGTACAATTTGGCATGCAGACCTCC
ATGTCACGCAAGGGGAACTGCTTCGACAATGCCCCGATAGAAAGCTTCTGGGGGTCTCTTAAAAATGAACTGGTTCACCACCATAGATTCGAGACGAGAG
CGGAGGCCAAGGCAGCGATTCAGGAATACATAGAGATTTTTTACAACCGCCAGCGTCGGCATTCACGACTTGGCAATGTCAGCCCGGCGGAGTTTAACAA
GAGGTATTGGAGAACTGCTCAGGCTGCTTGATCCGGGACTGTCCGATCTTGTCAGGACACCTCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
312 bp103 aa63374+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MKSREHYSPEFRAEAVRLVLEQGLGQAEAARRLGIAKGTLSNWVVAAKGGKDTSAIPGARSVAELEAEVARLRKALAQANMERDIIKKAAAYFAQESLPS
TRG

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
966 bp321 aa2661231+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

SSPATQSSRTSQHGARHHKKGRRVLCSGVTAKYAWIANQQDIYPISVICRVLEVSRAGFYHWRQRPPSQRQQEDERLKVAIRAAHSKTRQTYGASRLQAE
LAAEGFYAGRDRIGRLRREMGLSCKQKRKFKATTHSAHSLPVAENLLGQVFAPTKPNEVWTGDITYIATDEGWLYLAGLKDVFTCEIVGYAMGERMTTGL
VSQALFRAVQQKRPPVGLIHHTDRGSQYCAKAYRALQVQFGMQTSMSRKGNCFDNAPIESFWGSLKNELVHHHRFETRAEAKAAIQEYIEIFYNRQRRHS
RLGNVSPAEFNKRYWRTAQAA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1169 bp389 aa631231+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKSREHYSPEFRAEAVRLVLEQGLGQAEAARRLGIAKGTLSNWVVAAKGGKDTSAIPGARSVAELEAEVARLRKALAQANMERDIIKKGRRVLCSGVTAK
YAWIANQQDIYPISVICRVLEVSRAGFYHWRQRPPSQRQQEDERLKVAIRAAHSKTRQTYGASRLQAELAAEGFYAGRDRIGRLRREMGLSCKQKRKFKA
TTHSAHSLPVAENLLGQVFAPTKPNEVWTGDITYIATDEGWLYLAGLKDVFTCEIVGYAMGERMTTGLVSQALFRAVQQKRPPVGLIHHTDRGSQYCAKA
YRALQVQFGMQTSMSRKGNCFDNAPIESFWGSLKNELVHHHRFETRAEAKAAIQEYIEIFYNRQRRHSRLGNVSPAEFNKRYWRTAQAA

 

Blast result :
Comments
ISAeme20 is 78% aa similar to ISGlo1.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Pfeiffer F. (2015) Direct submission
2] Chai B., Wang H. and Chen X. (2012) J. Bacteriol. 194 (23), 6693-6694