ISAba34
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| KU744946 | ND | Acinetobacter baumannii | Acinetobacter baumannii strain A297 (RUH875) plasmid pA297-3 Acinetobacter sp. DUT-2 Acinetobacter sp. DUT-2 plasmid unnamed1 Acinetobacter baumannii strain AB031 |
DNA section
IS Length : 1309 bp
Ends
IR Length : 26
IRL : TGAACCGTACCGGGTTTGTCGGAGACTCAGTATTCTGAGAGAATGTTCCG
IRR : TGAACCGTACCGGGTTTGTCGGAGACATTTTTATTTAAGTTAGGCCACGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTTATTCGTT | AAG | TTTAGATAAG | 3 |
DNA sequence
TGAACCGTACCGGGTTTGTCGGAGACTCAGTATTCTGAGAGAATGTTCCGATGACCAAATTAAAATATACCCCTGAAATCAGAGAAAGAGCGGTTCAATT
ATTGATTGAATCTGAAAAAGACTATCCATCTAATTGGGCTGCGATCACAGCTATTGCGCCTAAGATTGGTTGTACTCCTGAAACACTTCGTGCTTGGCAT
CAAAAGCATTTAGATGAGCAAAATCCTATCAAAGTACAACAGATCTCTGATCAAGAAAAAATGAAGCAAATGGAACGTGAAATCAAAGAATTAAAGCGTG
CCAATGAAATTCTGCGTAAAGCAGCCGCTTTTTTCGCCCAGGCGGAGCTCGACCGCCCACACAAATAATGGTGGATTTCATCCATAACAATAAGGCGTTA
TATGGTGTTGAGGCGATTTGTAGGATTTTACCGATCTCAGCTTCTACCTATTATCGAACACTAGATATTGCTGAAAACCCAGAACATCGAGCAAAGCGAG
ATCTACATGATTTGCATCATGCTGAACAAATTAAACGAATTTGGAAAGAAAGTTCAGGTCGATATGGTGTACGTAAAGTTTGGCAACAACTGAAACGTGA
AGGTTATGTTATTGCACGCTGTACAGTGGTTCGCTTGATGAAGAAGCTAGGTATACAGGGTGTTTGGCGTGGGAAGAATAAATACACCACCCGTAGCCGA
GATGATCAAAAAAGAGCGGATGATTTAGTGAAACGTAATTTTACTGCTGATTATCCAAACCAACTATGGGTCGGTGACTTCACGTATATTCAAACTCATT
CAGGCTGGGTCTATACCGCCTTTATTATTGATGTGTTCTCACGAGCGATTGTTGGATGGAAAGTATCAACACGTATGAATACAGACATGGTGCTCGATGC
ACTAGAGCAAGCATTGCATGACCGAGGTATGCCTAAGAATGTGATTCATCATTCAGATAGAGGTGTTCAATATCTTTCAATTCGCTATACCAATCGTTTA
GAAGCAGCAAATTTACGAGCATCAGTCGGTACGACCGGTGATTCATACGATAACGCTTTGGCTGAAACAGTGAATGGGTTATACAAAACAGAGGTGATTG
AATATTTAAAAGCAGACTGGCAAGGTTTAGCGGATGTACAACTTGCGACATTAAATTGGGTAGATTGGTTCAACAAAAAGCGTGTACACAGCGCATTGGG
TTATGTATCGCCTTTTGAGTTTGAAGCAATGTACTATGATAAGATTAACCCGTTAGGTCACGTGGCCTAACTTAAATAAAAATGTCTCCGACAAACCCGG
TACGGTTCA
ATTGATTGAATCTGAAAAAGACTATCCATCTAATTGGGCTGCGATCACAGCTATTGCGCCTAAGATTGGTTGTACTCCTGAAACACTTCGTGCTTGGCAT
CAAAAGCATTTAGATGAGCAAAATCCTATCAAAGTACAACAGATCTCTGATCAAGAAAAAATGAAGCAAATGGAACGTGAAATCAAAGAATTAAAGCGTG
CCAATGAAATTCTGCGTAAAGCAGCCGCTTTTTTCGCCCAGGCGGAGCTCGACCGCCCACACAAATAATGGTGGATTTCATCCATAACAATAAGGCGTTA
TATGGTGTTGAGGCGATTTGTAGGATTTTACCGATCTCAGCTTCTACCTATTATCGAACACTAGATATTGCTGAAAACCCAGAACATCGAGCAAAGCGAG
ATCTACATGATTTGCATCATGCTGAACAAATTAAACGAATTTGGAAAGAAAGTTCAGGTCGATATGGTGTACGTAAAGTTTGGCAACAACTGAAACGTGA
AGGTTATGTTATTGCACGCTGTACAGTGGTTCGCTTGATGAAGAAGCTAGGTATACAGGGTGTTTGGCGTGGGAAGAATAAATACACCACCCGTAGCCGA
GATGATCAAAAAAGAGCGGATGATTTAGTGAAACGTAATTTTACTGCTGATTATCCAAACCAACTATGGGTCGGTGACTTCACGTATATTCAAACTCATT
CAGGCTGGGTCTATACCGCCTTTATTATTGATGTGTTCTCACGAGCGATTGTTGGATGGAAAGTATCAACACGTATGAATACAGACATGGTGCTCGATGC
ACTAGAGCAAGCATTGCATGACCGAGGTATGCCTAAGAATGTGATTCATCATTCAGATAGAGGTGTTCAATATCTTTCAATTCGCTATACCAATCGTTTA
GAAGCAGCAAATTTACGAGCATCAGTCGGTACGACCGGTGATTCATACGATAACGCTTTGGCTGAAACAGTGAATGGGTTATACAAAACAGAGGTGATTG
AATATTTAAAAGCAGACTGGCAAGGTTTAGCGGATGTACAACTTGCGACATTAAATTGGGTAGATTGGTTCAACAAAAAGCGTGTACACAGCGCATTGGG
TTATGTATCGCCTTTTGAGTTTGAAGCAATGTACTATGATAAGATTAACCCGTTAGGTCACGTGGCCTAACTTAAATAAAAATGTCTCCGACAAACCCGG
TACGGTTCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 318 bp | 105 aa | 51 | 368 | + | No |
Description : First part of the transposase
ORF sequence :
MTKLKYTPEIRERAVQLLIESEKDYPSNWAAITAIAPKIGCTPETLRAWHQKHLDEQNPIKVQQISDQEKMKQMEREIKELKRANEILRKAAAFFAQAEL
DRPHK
DRPHK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 951 bp | 316 aa | 320 | 1270 | + | No |
Description : Second part of the transposase
ORF sequence :
SSRFFRPGGARPPTQIMVDFIHNNKALYGVEAICRILPISASTYYRTLDIAENPEHRAKRDLHDLHHAEQIKRIWKESSGRYGVRKVWQQLKREGYVIAR
CTVVRLMKKLGIQGVWRGKNKYTTRSRDDQKRADDLVKRNFTADYPNQLWVGDFTYIQTHSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALH
DRGMPKNVIHHSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKKRVHSALGYVSPFE
FEAMYYDKINPLGHVA
CTVVRLMKKLGIQGVWRGKNKYTTRSRDDQKRADDLVKRNFTADYPNQLWVGDFTYIQTHSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALH
DRGMPKNVIHHSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKKRVHSALGYVSPFE
FEAMYYDKINPLGHVA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1220 bp | 406 aa | 51 | 1270 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKLKYTPEIRERAVQLLIESEKDYPSNWAAITAIAPKIGCTPETLRAWHQKHLDEQNPIKVQQISDQEKMKQMEREIKELKRANEILRKAAAFFRPGGA
RPPTQIMVDFIHNNKALYGVEAICRILPISASTYYRTLDIAENPEHRAKRDLHDLHHAEQIKRIWKESSGRYGVRKVWQQLKREGYVIARCTVVRLMKKL
GIQGVWRGKNKYTTRSRDDQKRADDLVKRNFTADYPNQLWVGDFTYIQTHSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALHDRGMPKNVIH
HSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKKRVHSALGYVSPFEFEAMYYDKIN
PLGHVA
RPPTQIMVDFIHNNKALYGVEAICRILPISASTYYRTLDIAENPEHRAKRDLHDLHHAEQIKRIWKESSGRYGVRKVWQQLKREGYVIARCTVVRLMKKL
GIQGVWRGKNKYTTRSRDDQKRADDLVKRNFTADYPNQLWVGDFTYIQTHSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALHDRGMPKNVIH
HSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKKRVHSALGYVSPFEFEAMYYDKIN
PLGHVA
Blast result :
Comments
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The transposase of ISAba34 is 96% aa similar to the transposase of ISAba19.
The transposase of ISAba34 is 96% aa similar to the transposase of ISAba19.
References
[1] Hamidian M., Ambrose S.J. and Hall R.M. (2015) Direct submission