ISfinder Mirror Site

ISAbc1

Family IS1595
Group ISSod11

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
MNKO01000038	ND	Acidobacteria bacterium	Acidobacteria bacterium 13_1_20CM_2_57_8

DNA section

IS Length : 1056 bp

Ends

IR Length : 30/34

IRL : GTTGGCTTATGCTTATACGGTTGTTTCAGCAAACCACGACCGGTAGTATG
IRR : GTCGCCTTCTGCTTATACGGTTGTTTCAGCAGACCCCAATAGATTGCCGC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GCGAACACGATAA		TAGAGCGAGGGT	0

DNA sequence

GTTGGCTTATGCTTATACGGTTGTTTCAGCAAACCACGACCGGTAGTATGAGCACGTGTCTCGCCCGCCGTTTCCGAAGACGTTGCGCGAATTCCAGACG
AAGTTCGCCAGCGATGAAGCGTGCCAGCAATACCTGGCCGCGTGTCGCTGGCCCGATGGCTTCGTTTGTCCACGATGCGGACAACGGCGAGCATACGAGT
TGGTGCAGCTGCGGCGTTGGCAGTGCGCCGGTTGTCGGCATCAAGTTTCGCTGACGGCAGGAACGATCCTGCATAACACGAAAATTGCGCTGACGGTTTG
GTTCTGGGCCGCCTACCTGATGACGACGGATAAGCGCGGCCTCTCCGCCCTGCTCTTGCAACGGCAGCTTGCCTTGGGACGTTATGAGACGGCCTGGATG
ATGCTCCATAAATTCCGGCGCGCAATGGTCAATCTGGTACGGGAGCCACTGCAAGGCGAAGTGGAAGTTGATGATACGTGGATTGGAGGTGAGCAGGCAG
GCTTGCGCGGGAGCCGGCAACTGAAAGGTCGGAAAGCGGCGCTCGTCCTTGTTGCAGTGGAGAAACGAGGCCGCGCAACCGGCCGCGCTCGGATGGCCGT
CATCCCGGATTTCAAGAGCGCCACTCTACTCGCTTTCCTCAAACAAAACGTGGCACCAGGTGCAACCGTATACACCGATGGCCTCAAAAGCTTCACGGGA
TTACAGGAAGCTGGATTCGAGCACGTGCCTCGAATTCAACCGCTGCGTATTGATCTGCGTAAAGGCGCAAAATCCGTGGTGCCCTTAGCTGACCGCGCAA
TTGGCAATCTGCAACAATGGCTGATCGGAACCCATCACGGCGTGAGCCGCGGTCAGCTTCCGGTTTATCTCGACGAATTCGTCTTCCGGCATAATCGCCG
AAAGCACCCCATGGCTGCATTCCAGACCTTGCTCGGTCTGGGAACCGCGCGCAATCCAACGCCTTACGATAAAATACGCGGCGCGGCTGATCTTTCTGCA
AACTAAGCGGCAATCTATTGGGGTCTGCTGAAACAACCGTATAAGCAGAAGGCGAC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
951 bp	316 aa	56	1006	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSRPPFPKTLREFQTKFASDEACQQYLAACRWPDGFVCPRCGQRRAYELVQLRRWQCAGCRHQVSLTAGTILHNTKIALTVWFWAAYLMTTDKRGLSALL
LQRQLALGRYETAWMMLHKFRRAMVNLVREPLQGEVEVDDTWIGGEQAGLRGSRQLKGRKAALVLVAVEKRGRATGRARMAVIPDFKSATLLAFLKQNVA
PGATVYTDGLKSFTGLQEAGFEHVPRIQPLRIDLRKGAKSVVPLADRAIGNLQQWLIGTHHGVSRGQLPVYLDEFVFRHNRRKHPMAAFQTLLGLGTARN
PTPYDKIRGAADLSAN

Blast result :

Comments

ISAbc1 is 65% aa similar to ISRop3.

References

1] ISfinder annotation (2017)
2] Butterfield,C.N., Li,Z., Andeer,P.F., Spaulding,S., Thomas,B.C., Singh,A., Hettich,R.L., Suttle,K.B., Probst,A.J., Tringe,S.G., Northen,T., Pan,C. and Banfield,J.F. ( 2016) PeerJ 4, E2687.