ISAni1
- Family IS481
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| X97015 | ND | Arthrobacter nicotinovorans | Arthrobacter nicotinovorans plasmid pAO1 |
DNA section
IS Length : 1075 bp
Ends
IR Length : 23/27
IRL : TGTATTGACCACGGACGTTAGTGACGCTCGGCGTGGTTTGGTGACATGAA
IRR : TGTATTGACCTGAGACGTTGGTGACGCGGCTGGCTGGCGTCTGACCTTTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGTGGATCTT | ACTAGT | TGCTGCCGGG | 6 |
DNA sequence
TGTATTGACCACGGACGTTAGTGACGCTCGGCGTGGTTTGGTGACATGAAGAAGACCTCCGGGTGGAGTGGGGCTTGTCTAGAGTCCTTTTCCACACACG
GAGGTCTTCGTGTCCCACCCTAACGCTTTCCTCACTCCTCGTGGCCGGTTGCAGCTCGCGCAGTGCGTTGTTGATCAGTGCTGGAGCCTGCGCCGTGCCG
GAGCGGTTTCAGGGGTCTCGATCCCGACCGCTGAACGTTGGGCGAAGCGCTACCTCGAGGAGGGACCCGAAGGAATGGAGGACCGTTCGAGTCGGCCACA
TTCCTCGCCCCGCGGGACTGCTACGCGTACCGAGCGGAGGGTCATCGCCGTGCGCGTGAACCGCCGGTGGGGCCCTGCCAGGATCGCGGCTATTTGCTGG
GCCTGCACCCCTCAACCGTCCACCGGGTACTGTCCCGCCACCGGCTGGCGAAACTAACCTGGCTCGATCGCGGCACAGGACGAGTGATCCATCGCTATGA
ACACGACAGGCCCGGGGATTTGGTCCACCTGGACATCAAGAAGCTGGGCCGGATCCCCGACGGCGGCGGACACCGAGTCATGGGCAGGACCGCCGGCTGG
AAGAACAAGACCGGCACCAAGACCAACCGCCGGCCTGGCTACCACTACCTCCACAATGCCGAGGATGACCACTCACGACTGGCTTACACCGAGATCCTCA
CTGACGAAACCAAGGAAACAGCGGCAGACTTCTGGGTGCGGGCAAACGCCTGGTTCAAAGAACACGGAGTTACCGTCCAGCGTGTTCTGGCTGACAACGG
GAACTGCTACCGGTCTCATGCTTACGCCGAAGCGCTAGGGCCGGACATGAAACACAAACGCACCCGCCCCTACAGGCCACAAACCAACGGCAAAGTCGAA
CGGTCCAACGGCACCATGCTCGAGGAATGGGCCTACATCCGCCCCTACCGCTCAGAAGCCGAGCGGGTTGCTGCTTTCCCCGACTGGCTCCATGCCTACA
ATCACCATCGAGCCAACACTGCGCTCAAAGGTCAGACGCCAGCCAGCCGCGTCACCAACGTCTCAGGTCAATACA
GAGGTCTTCGTGTCCCACCCTAACGCTTTCCTCACTCCTCGTGGCCGGTTGCAGCTCGCGCAGTGCGTTGTTGATCAGTGCTGGAGCCTGCGCCGTGCCG
GAGCGGTTTCAGGGGTCTCGATCCCGACCGCTGAACGTTGGGCGAAGCGCTACCTCGAGGAGGGACCCGAAGGAATGGAGGACCGTTCGAGTCGGCCACA
TTCCTCGCCCCGCGGGACTGCTACGCGTACCGAGCGGAGGGTCATCGCCGTGCGCGTGAACCGCCGGTGGGGCCCTGCCAGGATCGCGGCTATTTGCTGG
GCCTGCACCCCTCAACCGTCCACCGGGTACTGTCCCGCCACCGGCTGGCGAAACTAACCTGGCTCGATCGCGGCACAGGACGAGTGATCCATCGCTATGA
ACACGACAGGCCCGGGGATTTGGTCCACCTGGACATCAAGAAGCTGGGCCGGATCCCCGACGGCGGCGGACACCGAGTCATGGGCAGGACCGCCGGCTGG
AAGAACAAGACCGGCACCAAGACCAACCGCCGGCCTGGCTACCACTACCTCCACAATGCCGAGGATGACCACTCACGACTGGCTTACACCGAGATCCTCA
CTGACGAAACCAAGGAAACAGCGGCAGACTTCTGGGTGCGGGCAAACGCCTGGTTCAAAGAACACGGAGTTACCGTCCAGCGTGTTCTGGCTGACAACGG
GAACTGCTACCGGTCTCATGCTTACGCCGAAGCGCTAGGGCCGGACATGAAACACAAACGCACCCGCCCCTACAGGCCACAAACCAACGGCAAAGTCGAA
CGGTCCAACGGCACCATGCTCGAGGAATGGGCCTACATCCGCCCCTACCGCTCAGAAGCCGAGCGGGTTGCTGCTTTCCCCGACTGGCTCCATGCCTACA
ATCACCATCGAGCCAACACTGCGCTCAAAGGTCAGACGCCAGCCAGCCGCGTCACCAACGTCTCAGGTCAATACA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 971 bp | 323 aa | 110 | 1080 | + | No |
Chemistry : DDE
ORF sequence :
MSHPNAFLTPRGRLQLAQCVVDQCWSLRRAGAVSGVSIPTAERWAKRYLEEGPEGMEDRSSRPHSSPRGTATRTERRVIAVRVNRRWGPCQDRGYLLGLH
PSTVHRVLSRHRLAKLTWLDRGTGRVIHRYEHDRPGDLVHLDIKKLGRIPDGGGHRVMGRTAGWKNKTGTKTNRRPGYHYLHNAEDDHSRLAYTEILTDE
TKETAADFWVRANAWFKEHGVTVQRVLADNGNCYRSHAYAEALGPDMKHKRTRPYRPQTNGKVERSNGTMLEEWAYIRPYRSEAERVAAFPDWLHAYNHH
RANTALKGQTPASRVTNVSGQYN
PSTVHRVLSRHRLAKLTWLDRGTGRVIHRYEHDRPGDLVHLDIKKLGRIPDGGGHRVMGRTAGWKNKTGTKTNRRPGYHYLHNAEDDHSRLAYTEILTDE
TKETAADFWVRANAWFKEHGVTVQRVLADNGNCYRSHAYAEALGPDMKHKRTRPYRPQTNGKVERSNGTMLEEWAYIRPYRSEAERVAAFPDWLHAYNHH
RANTALKGQTPASRVTNVSGQYN
Blast result :
Comments
ISAni1 is 63% similar to IS1652 and IS1649, 64% to IS5564, 61% to ISSco1and 57% to U85507.
There is a -1 frameshift at position 375 which has been "corrected" in the translation of the transposase.
Note that the transposase termination codon is generated by sequence specific insertion.
There is a -1 frameshift at position 375 which has been "corrected" in the translation of the transposase.
Note that the transposase termination codon is generated by sequence specific insertion.
References
1] Menendez,C.U. (1996) Direct Submission GenBank.