ISAba43

  • Family ISL3
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Acinetobacter baumannii
Acinetobacter baumannii Ab825
Acinetobacter baumannii Ab242
DNA section
IS Length : 2655 bp

Ends


IR Length : 65

IRL : GTTCTTTGCACTTTAGCGTGAAGATTGTTACGATATGGACTCATCCATGA
IRR : GTTCATCGCACTTTGCCGGGAAATGCAGCACGGATCTTGAGGAAGAAGTA

Insertion site


Left flankDirect repeatRight flankDR Length
ttatttaatccttataaatttcgttactta10
tgggtgtctattaatttactagatttct8

DNA sequence

GTTCTTTGCACTTTAGCGTGAAGATTGTTACGATATGGACTCATCCATGAGATAACCCGCACATGAGCGCTACCAAGAAAGCAGACAAGTAAGCCGCAGC
AACCTTCGTTTTCGGTTGTTGCGGCGTTCTCAAGAACCTAGTTGCTCTGCAAGAGCGCCGCCAAATTCTTAACTTAAGGAGATGGCTTCGTGCGCTCAAA
AGATTTTTCTTGGCGGTATTCTCTTCCCGCCACGCTATTGCTGTTATCACCATTCGATTTGCTGGCGTCACTGGGCATGGATATGTACCTGCCCGTGGTG
CCTTTCATGGCGGATGCACTTGGGTCCGGTGCAGGGACGATCCAGCTGACGTTGACGGCATACCTGGTTTTGCTTGGAGCCGGACAGCTTCTCTTCGGCC
CACTGTCGGATCGGCTGGGTCGCCGCCCCGTTTTACTTGGTGGCGGGATTGCCTATATTGCGGCTTCATTCGGCCTCACCGTAGTTTCATCGCCAGAACT
TTTTCTGAGCTTCCGCGTTCTTCAGGCCTGCGGCGCTTCGGCATGTCTCGTTTCCACTTTCGCGACGGTACGCGACATCTATTCGGGCCGTGAGGAAAGC
AACGTCATCTATGGCTTGCTCGGCTCTATGCTTGCGATGGTTCCTGCGATAGGCCCACTGCTAGGAGCGCTGGTCGACGCGTGGCTCGGATGGCGCGCAA
TCTTCGGCTTGCTAGGGATGGCAATGATAGGCGCTGTTATCGCAGCCTGGCGACTCTGGCCAGAGACTCGGCGGCACCGGACGGCAGACCTACAGTGGTC
GCAGCTATTGACTCCTGTGAAACACCTGAACTTCTGGCTGTACACGCTCTGTTACAGCGCGGGGATGGGCAGCTTTTTCGTCTTTTTCTCGACCGCCCCC
TGGCTAATGATGGGGAGGCAAGGGTTATCGCAACTTAGCTTCAGCTTGCTATTTGCTACAGTGGCCATCGCTATGATGGCTACAGCGCGAATCATGGGAC
GACTGATTCCCCGGTGGGGAAGCCTGAAGACCTTACGAGTGGGAATGGGGTGTCTGATGGCGGGAGCGCTGTTGCTCGCTGTTGGCGAGACATTGGCACC
GGTCTCGGTGCTTGGCTTCATCGCCCCGATGTGGCTCGTGGGTGTTGGTATCGCCACTGCGGTATCGGTGGCACCCAATGCCGCTCTTCGAGGTTTCGAT
CATATCGCTGGGACCGCTACAGCAGTCTACTTCTGTTTGGGTGGACTACTGTTAGGGATTATCGGGACACTCATTATTACGCTTTTATCGACTGGTACCA
CCTGGCCGATCATCGCTTATTGCCTCATCCTCGCAACAGCAGTGCTTTGTCTGTCCTGCATCAATCCCAACAGACGCCATCTCAGCCAAGAAGAACATGA
TGCCTTGGCGCTACAAGGCACCGATAGCGCGCAATCGGTCCATGATCATGACTAGCGCTGATGTTTGGAGCCTGTTCTGGAAGGGCTTTTCGCCAGCGTC
TCATGGGCTTCTAGATGAACAGACTTTGCTGATCCGTCTTACACACGACGAGGAGCATACGCCAATTTGTAGCCGATGCGATTGCCCTTGTTTCCTGGTA
CATGACATCAATTGGCGCCGAGTTCGCGAGTCACCAATTATGCAGTATCGCGTCGAGTTGGATGTACCTGTGCGGCGGCTGCGTTGTCCACAATGCGGTC
CAACCAGAGAGCGGATCGATTGGCTACCACCGCGCTCCCGTATTACGCAATCATTGCGCAACTGGATTGAGAAATTAGTACAGATGCTGCCCGTCAGTCA
TGTTGCCAATCTTTTGGGCTTGCATTGGCATACAGTCAAGACTATTGACATGGAGCGGCTCAAACGTGATTTGCACGAGCCAGATCGAAGTCGATTGCGC
CGCCTGATGATGGACGAATTCGCCTTGCACAAGGGTCATCGCTACGCCACGGTGGTCGCCTGTGCCGACACCCAGCAAGTGGTGTGGATTGGCGAGGGCC
GCTCGCGTGAGGCGATCCGGCCGTTCTTCGAGTGGTTAGGCGATGCCCGGAACCAGATCGAGGCAGTGGCCATGGACATGAACTCAGCCTTCGATCTTGA
AGTGAAGGCCCAGTGCCCCAACGCGGTGGTGGTCTACGACCTGTTCCATGTGGTGGCTAAGTATGGCCGCGAAGTGATGGATAGAGTGCGAGTTGATCAG
GCCAATCAACTGCGAGATGACAAGGTCGCCCGCAAGGTGATCAAAAGCAGCCGTTGGATACTGTTACGCAATGCTGACAACCTTGAGCCCGAGCAGGCGG
TCAAGCTGGACGAACTCCTGGCCGCCAATGCGTTCTTGACTACGGTGTACGTGCTCAAGGACCAACTTAAGACGCTATGGTTTGCGGAAAGCGAGACGAC
GGCCAGAAGCGCCTGGCGGGAATGGGCCGGCATGGCCCTCGGTAGCGGAATTGATGCTTTGGTACGATTTGCCAAGAAGCTGGAGCCATATATGGAGGGT
ATCGTTTCCAGTGCCCTCCACCGGCTGAACACCAGCGTGCTGGAGGGCATGAACAACCGGATCAAAGTCATTAAGCGGATGGCCTATGGATACCGCGACA
CGGACTACTTCTTCCTCAAGATCCGTGCTGCATTTCCCGGCAAAGTGCGATGAAC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1266 bp421 aa1901455+No
ORF function : Passenger Gene
Annotation : Drug resistance transporter, Bcr/CflA subfamilyDescription : Antibiotic resistance

ORF sequence :

MRSKDFSWRYSLPATLLLLSPFDLLASLGMDMYLPVVPFMADALGSGAGTIQLTLTAYLVLLGAGQLLFGPLSDRLGRRPVLLGGGIAYIAASFGLTVVS
SPELFLSFRVLQACGASACLVSTFATVRDIYSGREESNVIYGLLGSMLAMVPAIGPLLGALVDAWLGWRAIFGLLGMAMIGAVIAAWRLWPETRRHRTAD
LQWSQLLTPVKHLNFWLYTLCYSAGMGSFFVFFSTAPWLMMGRQGLSQLSFSLLFATVAIAMMATARIMGRLIPRWGSLKTLRVGMGCLMAGALLLAVGE
TLAPVSVLGFIAPMWLVGVGIATAVSVAPNAALRGFDHIAGTATAVYFCLGGLLLGIIGTLIITLLSTGTTWPIIAYCLILATAVLCLSCINPNRRHLSQ
EEHDALALQGTDSAQSVHDHD

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1206 bp401 aa14482653+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTSADVWSLFWKGFSPASHGLLDEQTLLIRLTHDEEHTPICSRCDCPCFLVHDINWRRVRESPIMQYRVELDVPVRRLRCPQCGPTRERIDWLPPRSRIT
QSLRNWIEKLVQMLPVSHVANLLGLHWHTVKTIDMERLKRDLHEPDRSRLRRLMMDEFALHKGHRYATVVACADTQQVVWIGEGRSREAIRPFFEWLGDA
RNQIEAVAMDMNSAFDLEVKAQCPNAVVVYDLFHVVAKYGREVMDRVRVDQANQLRDDKVARKVIKSSRWILLRNADNLEPEQAVKLDELLAANAFLTTV
YVLKDQLKTLWFAESETTARSAWREWAGMALGSGIDALVRFAKKLEPYMEGIVSSALHRLNTSVLEGMNNRIKVIKRMAYGYRDTDYFFLKIRAAFPGKV
R

 

Blast result :
Comments
ISAba43 (transposase) is 69% aa similar to the transopsase of ISAeme19.
References
1] Repizo GD (2017) Direct submission to ISfinder
2] Mussi MA, Limansky AS, Viale AM. (2005) Antimicrob Agents Chemother. Apr;49(4):1432-40