ISAeca6
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Aeromonas caviae | Aeromonas caviae |
DNA section
IS Length : 1198 bp
Ends
IR Length : 11/12
IRL : GGTAATCCCCCCGAAAAACTGAGGTGCTCATAAGTAGAATTTTCCCGTAC
IRR : GGTATTCCCCCCATTCTTAGGGGCACTCAAAAGTAGAGGTCATGCGGCCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGTGGTAGTG | TTAT | CATCAATAAT | 4 |
DNA sequence
GGTAATCCCCCCGAAAAACTGAGGTGCTCATAAGTAGAATTTTCCCGTACCTTGATACCCGGAGGTTCTACATGAAAACATCACGCTTCAGCGATGCCCA
GATCATCGCCATTCTTAACCAGGCCGAGGCTGGCTCCCCCGTTCCAGAGCTCTGCCGCGAACATGGCATCAGCTCCGCCACCTTCTACAAGTGGCGCTCT
AAATACGGCGGAATGGATGCCTCCCTCATGGCTCGCCTCAAAGAGCTGGAGGACGAGAACCGCCGCCTCAAGAAGATGTACGCCGAGGAGCGCCTCAAAG
CCGAGATCATCGCTGAGGCCATGGCAAAAAAGTGGTGACGCCATCTGCCCGCCGGGAGATGGCAACCAGGGCCGTAGCGGATTACGCCATCTCCATCCGC
CGGGCTTGCCAGGTGTTCAAGGTCAGTGAGGGCTGCTATCGCTATCAGCCGGTGCTGGCCGATGAAAATCAGGAGATTGCCGATTGGCTGCTGCGCCTCA
CCGCTAATCAGCGTAATTGGGGTTTTGGACTGTGCTTTCTGTACCTGCGTAACGTCAAAGGCTTTGGCTGGAACCACAAGCGGGTGTACCGGATTTATCG
CGCCCTTGAACTTAATCTGAGGATAAAGCCGCGAAAACGGCTGGTACGGGCCAAACCGGAGCCGCTGGCGGTCCCTGAAAAACCCAATCACTGCTGGTCG
ATGGACTTCATGCATGACCAGCTCGGTGATGGCCGTTCGGTGCGGCTGTTTAATGTGATCGACGACTTCAACCGCGAAGCACTGTGCATCGAAGTGGATT
TCTCCTTGCCTGCCACACGGGTCAAACGGGCGCTGGAGCAAGTGATCGCGTGGCGGGGAAAGCCGGTGGAGATCCGCTGCGATAATGGCCCGGAATATAT
CAGCGAGGAGCTCAAGCGCTGGGCTCAGCAGGAAGGGATAGCGCTACGGTATATCCAGCCGGGCAATCCCCAACAGAACGCGTATATCGAGCGGTTTAAC
CGCACGGTACGTTACGACTGGCTGGGTCATTACCTGTTTGAGTCACTGAATGAATTGCAGGCATTTGCCACAAACTGGTTGTGGACGTATAACCATGAGC
GGCCCAATATGGCGCTGGGTGGCTACACACCAAAACAGCGCCTAATGCAGGCCGCATGACCTCTACTTTTGAGTGCCCCTAAGAATGGGGGGAATACC
GATCATCGCCATTCTTAACCAGGCCGAGGCTGGCTCCCCCGTTCCAGAGCTCTGCCGCGAACATGGCATCAGCTCCGCCACCTTCTACAAGTGGCGCTCT
AAATACGGCGGAATGGATGCCTCCCTCATGGCTCGCCTCAAAGAGCTGGAGGACGAGAACCGCCGCCTCAAGAAGATGTACGCCGAGGAGCGCCTCAAAG
CCGAGATCATCGCTGAGGCCATGGCAAAAAAGTGGTGACGCCATCTGCCCGCCGGGAGATGGCAACCAGGGCCGTAGCGGATTACGCCATCTCCATCCGC
CGGGCTTGCCAGGTGTTCAAGGTCAGTGAGGGCTGCTATCGCTATCAGCCGGTGCTGGCCGATGAAAATCAGGAGATTGCCGATTGGCTGCTGCGCCTCA
CCGCTAATCAGCGTAATTGGGGTTTTGGACTGTGCTTTCTGTACCTGCGTAACGTCAAAGGCTTTGGCTGGAACCACAAGCGGGTGTACCGGATTTATCG
CGCCCTTGAACTTAATCTGAGGATAAAGCCGCGAAAACGGCTGGTACGGGCCAAACCGGAGCCGCTGGCGGTCCCTGAAAAACCCAATCACTGCTGGTCG
ATGGACTTCATGCATGACCAGCTCGGTGATGGCCGTTCGGTGCGGCTGTTTAATGTGATCGACGACTTCAACCGCGAAGCACTGTGCATCGAAGTGGATT
TCTCCTTGCCTGCCACACGGGTCAAACGGGCGCTGGAGCAAGTGATCGCGTGGCGGGGAAAGCCGGTGGAGATCCGCTGCGATAATGGCCCGGAATATAT
CAGCGAGGAGCTCAAGCGCTGGGCTCAGCAGGAAGGGATAGCGCTACGGTATATCCAGCCGGGCAATCCCCAACAGAACGCGTATATCGAGCGGTTTAAC
CGCACGGTACGTTACGACTGGCTGGGTCATTACCTGTTTGAGTCACTGAATGAATTGCAGGCATTTGCCACAAACTGGTTGTGGACGTATAACCATGAGC
GGCCCAATATGGCGCTGGGTGGCTACACACCAAAACAGCGCCTAATGCAGGCCGCATGACCTCTACTTTTGAGTGCCCCTAAGAATGGGGGGAATACC
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 72 | 338 | + | No |
Description : First part of the transposase
ORF sequence :
ATGAAAACATCACGCTTCAGCGATGCCCAGATCATCGCCATTCTTAACCAGGCCGAGGCTGGCTCCCCCGTTCCAGAGCTCTGCCGCGAACATGGCATCA
GCTCCGCCACCTTCTACAAGTGGCGCTCTAAATACGGCGGAATGGATGCCTCCCTCATGGCTCGCCTCAAAGAGCTGGAGGACGAGAACCGCCGCCTCAA
GAAGATGTACGCCGAGGAGCGCCTCAAAGCCGAGATCATCGCTGAGGCCATGGCAAAAAAGTGGTGA
GCTCCGCCACCTTCTACAAGTGGCGCTCTAAATACGGCGGAATGGATGCCTCCCTCATGGCTCGCCTCAAAGAGCTGGAGGACGAGAACCGCCGCCTCAA
GAAGATGTACGCCGAGGAGCGCCTCAAAGCCGAGATCATCGCTGAGGCCATGGCAAAAAAGTGGTGA
Blast result :Comments : First part of the transposaseORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 843 bp | 280 aa | 317 | 1159 | + | No |
Description : Second part of the transposase
ORF sequence :
ATGGCAACCAGGGCCGTAGCGGATTACGCCATCTCCATCCGCCGGGCTTGCCAGGTGTTCAAGGTCAGTGAGGGCTGCTATCGCTATCAGCCGGTGCTGG
CCGATGAAAATCAGGAGATTGCCGATTGGCTGCTGCGCCTCACCGCTAATCAGCGTAATTGGGGTTTTGGACTGTGCTTTCTGTACCTGCGTAACGTCAA
AGGCTTTGGCTGGAACCACAAGCGGGTGTACCGGATTTATCGCGCCCTTGAACTTAATCTGAGGATAAAGCCGCGAAAACGGCTGGTACGGGCCAAACCG
GAGCCGCTGGCGGTCCCTGAAAAACCCAATCACTGCTGGTCGATGGACTTCATGCATGACCAGCTCGGTGATGGCCGTTCGGTGCGGCTGTTTAATGTGA
TCGACGACTTCAACCGCGAAGCACTGTGCATCGAAGTGGATTTCTCCTTGCCTGCCACACGGGTCAAACGGGCGCTGGAGCAAGTGATCGCGTGGCGGGG
AAAGCCGGTGGAGATCCGCTGCGATAATGGCCCGGAATATATCAGCGAGGAGCTCAAGCGCTGGGCTCAGCAGGAAGGGATAGCGCTACGGTATATCCAG
CCGGGCAATCCCCAACAGAACGCGTATATCGAGCGGTTTAACCGCACGGTACGTTACGACTGGCTGGGTCATTACCTGTTTGAGTCACTGAATGAATTGC
AGGCATTTGCCACAAACTGGTTGTGGACGTATAACCATGAGCGGCCCAATATGGCGCTGGGTGGCTACACACCAAAACAGCGCCTAATGCAGGCCGCATG
A
CCGATGAAAATCAGGAGATTGCCGATTGGCTGCTGCGCCTCACCGCTAATCAGCGTAATTGGGGTTTTGGACTGTGCTTTCTGTACCTGCGTAACGTCAA
AGGCTTTGGCTGGAACCACAAGCGGGTGTACCGGATTTATCGCGCCCTTGAACTTAATCTGAGGATAAAGCCGCGAAAACGGCTGGTACGGGCCAAACCG
GAGCCGCTGGCGGTCCCTGAAAAACCCAATCACTGCTGGTCGATGGACTTCATGCATGACCAGCTCGGTGATGGCCGTTCGGTGCGGCTGTTTAATGTGA
TCGACGACTTCAACCGCGAAGCACTGTGCATCGAAGTGGATTTCTCCTTGCCTGCCACACGGGTCAAACGGGCGCTGGAGCAAGTGATCGCGTGGCGGGG
AAAGCCGGTGGAGATCCGCTGCGATAATGGCCCGGAATATATCAGCGAGGAGCTCAAGCGCTGGGCTCAGCAGGAAGGGATAGCGCTACGGTATATCCAG
CCGGGCAATCCCCAACAGAACGCGTATATCGAGCGGTTTAACCGCACGGTACGTTACGACTGGCTGGGTCATTACCTGTTTGAGTCACTGAATGAATTGC
AGGCATTTGCCACAAACTGGTTGTGGACGTATAACCATGAGCGGCCCAATATGGCGCTGGGTGGCTACACACCAAAACAGCGCCTAATGCAGGCCGCATG
A
Blast result :Comments : Second part of the transposaseORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1088 bp | 362 aa | 72 | 1159 | + | Yes |
Chemistry : DDE
ORF sequence :
MKTSRFSDAQIIAILNQAEAGSPVPELCREHGISSATFYKWRSKYGGMDASLMARLKELEDENRRLKKMYAEERLKAEIIAEAMAKKVVTPSARREMATR
AVADYAISIRRACQVFKVSEGCYRYQPVLADENQEIADWLLRLTANQRNWGFGLCFLYLRNVKGFGWNHKRVYRIYRALELNLRIKPRKRLVRAKPEPLA
VPEKPNHCWSMDFMHDQLGDGRSVRLFNVIDDFNREALCIEVDFSLPATRVKRALEQVIAWRGKPVEIRCDNGPEYISEELKRWAQQEGIALRYIQPGNP
QQNAYIERFNRTVRYDWLGHYLFESLNELQAFATNWLWTYNHERPNMALGGYTPKQRLMQAA
AVADYAISIRRACQVFKVSEGCYRYQPVLADENQEIADWLLRLTANQRNWGFGLCFLYLRNVKGFGWNHKRVYRIYRALELNLRIKPRKRLVRAKPEPLA
VPEKPNHCWSMDFMHDQLGDGRSVRLFNVIDDFNREALCIEVDFSLPATRVKRALEQVIAWRGKPVEIRCDNGPEYISEELKRWAQQEGIALRYIQPGNP
QQNAYIERFNRTVRYDWLGHYLFESLNELQAFATNWLWTYNHERPNMALGGYTPKQRLMQAA
Blast result :
Comments
ISAeca6 is 97% aa similar to ISAs22.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] F. Pfeiffer (2017) Direct submission
2] M. Blettinger, M.A. Zamora-Lagos, F. Pfeiffer, B. Habermann, S. Gruber (in preparation)
2] M. Blettinger, M.A. Zamora-Lagos, F. Pfeiffer, B. Habermann, S. Gruber (in preparation)