ISAfd1

  • Family IS701
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
MF993499 Y Acidithiobacillus ferridurans
Acidithiobacillus ferridurans
Acidithiobacillus ferridurans ATCC 33020 plasmid pTF5
DNA section
IS Length : 1548 bp

Ends


IR Length : 20/21

IRL : CTGAAGTTTAGACATACAGCCCGGCTCCGCCGGGTTTTTTCTATTTTTGC
IRR : CTGAAGTTTAGACATGCAGCCTCCTCGCCAGCTTCTATGTGTGGCGATAT

Insertion site


Left flankDirect repeatRight flankDR Length
TAGAACGTGTGGGTATTGTTAACAACGCAACAACAT9
CTCAACTTCATATGGTTATAGAAGAGCGATGGTA8

DNA sequence

CTGAAGTTTAGACATACAGCCCGGCTCCGCCGGGTTTTTTCTATTTTTGCTACGAAAGTGCTTGACACAGTGATAGTGCCGTGCGGCCGACCCTTGTAAA
AAAGGTCGGCCAATGAGAAGCGTTTTAGGCTTCTCGGAATCACTGTCATGCCCTTTCATCGTCTCGTTGCCCTCCGCCTCTCGGAGTGGATTTCCTACTT
TCTGACGGTCGTGCCGCCCCGCTCGCGGCGCAGTTTTGTGGAGCTGCTTTGCGGTTGCCTGATCTCGCCCGAAGGCTGGGTCACCCGCGCCCTCAGCAGC
ATCTCCCTCCGCTGCCACTGGACCACCTATTACAAACTCCTGGAGCGTGCGTCCTTGCGTACACAGGCGTTGGCCATAACCCAGGTACGCTGGCTCCTGC
AGTCCTTGCCCAAGCCCCTGATCGTCGAGTTAGTCGTCGACGACACGATGGTCCTGCGGCACTCTACCAAGGCCCCAGGCGCGGCCATCCGCTTCGACCA
CAGTCATAAGCACAACCGCCCCAGTTATGTCCTCGCCCAGAATTGGGTGGGTCTGGCGTTGACCCTGCGTACCCGTTCCGGCAAAGCCATTTCCTTTCCC
ATCCGCTTGCGCCTGGTGCCCAGTACCGGCAACACCCACAAGCTGAAAATCGCCGGAGCATTGCTGCGCGCCTTTATACCCCACATCCCCGTACCCGTCC
GGTTGCTCACCGATGCTTGGTTCATGCGGCGGCGCCTGCTCTTGCCCCTGTTGCGTCAGCACGGCCTGCAGCCGGCAGGCCAGTTCATCGGCCAGGTGCG
CAAAGATACTGTACTGCTCCGCGACCCACCGCCGAGGACATCCCAACGCGGGCGTCCTCGGATATATGGAAACAAGTACACCGTGGAAGACATGGCTGCG
CTGCCCACCGAGATCGTCTCTCTGGATCTCTATGGGAAACGGCAGAAGGTCCGTCTTCAATCCATCATCGCCAATGCACGATTCCTGAACGGCCATCCGG
TCCGTGCCGTATGGAGTGCGATCTACGATGAAAAGCATCATCGGTGGTCTCCCACCCGCCTCTATCTCGCTTCCGAGACGGATCTCACCGCCAGCGAAAT
CGTCGTCCTCTACAGCAACCGCTGGCAGATCGAACCGATCTTCCACAATCTCAAACGCTGGTGGGGCATCCATAACCTCTGGCAGCAAAAACGCACCGTC
TTGGAACGCTGGATGCAAATCCGCTGCATCGCTTGGTCCATGGTGCAACTCCTCGCCGAAACCCTATCCGAAGATTTCCCCATGACTGCAGTAGCGCCCT
GGCGTATCGCTACTCCTGTCACCGCAGGACTCATGGCCCAATGGCTTCATCTGCAATTTCTGCGGGTTCCATTCTGGAAGGGCTACGACCCGAAGTCCGG
GAAATTCCAGTGCCCCGCCCCCGCCTTTTGGGCCGATACCGATGACCCACCCCGCAAAAAGGTCGCCTGACTCAATCCTGCTCCACATTTACTACGCCAT
ATCGCCACACATAGAAGCTGGCGAGGAGGCTGCATGTCTAAACTTCAG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1323 bp440 aa1481470+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPFHRLVALRLSEWISYFLTVVPPRSRRSFVELLCGCLISPEGWVTRALSSISLRCHWTTYYKLLERASLRTQALAITQVRWLLQSLPKPLIVELVVDDT
MVLRHSTKAPGAAIRFDHSHKHNRPSYVLAQNWVGLALTLRTRSGKAISFPIRLRLVPSTGNTHKLKIAGALLRAFIPHIPVPVRLLTDAWFMRRRLLLP
LLRQHGLQPAGQFIGQVRKDTVLLRDPPPRTSQRGRPRIYGNKYTVEDMAALPTEIVSLDLYGKRQKVRLQSIIANARFLNGHPVRAVWSAIYDEKHHRW
SPTRLYLASETDLTASEIVVLYSNRWQIEPIFHNLKRWWGIHNLWQQKRTVLERWMQIRCIAWSMVQLLAETLSEDFPMTAVAPWRIATPVTAGLMAQWL
HLQFLRVPFWKGYDPKSGKFQCPAPAFWADTDDPPRKKVA

 

Blast result :
Comments
ISAfd1 is 40% aa similar to ISMco3.
References
1] D. Barrie Johnson, Barry M. Grail and Violaine Bonnefoy. (2015) Adv. Mat. Res.
2] Violaine Bonnefoy, Barry M. Grail and D. Barrie Johnson. (2018) Appl Environ Microbiol. 2018.