ISAlw13
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Acinetobacter lwoffii | Acinetobacter lwoffii Acinetobacter lwoffii M2a |
DNA section
IS Length : 811 bp
Ends
IR Length : 17
IRL : GGGCGTGTCATCAATTACCTTGAGGAAAACCACCTTGTCCCCATTATACT
IRR : GGGCGTGTCATCAATTAATGGAGATGATTGTTGATGCTAAGTAGATCGCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ctcgaaatatggatcgaatc | ta | cataagatctataaaaagct | 2 |
| tgaaataaatcatggggccc | TA | gatagcacagcatcttattt | 2 |
DNA sequence
GGGCGTGTCATCAATTACCTTGAGGAAAACCACCTTGTCCCCATTATACTTTTATGACTAAACGCTATGCCTTAAGAGATGATCAATGGGAACAGATTAA
AGATCTGTTACCTGGACGAATGGGTACTGTTGGAGTAACGGCCAAAGACAACCGACTATTTGTTGAAGCAGTATTGTATCGGTATCGTTCAGGTATCCCG
TGGCGAGACTTACCAGAACGCTTTGGTGATTTTAGAGTCGTGCATACTCGTTTTAGCCGTTGGGCTAAAACAGGTGTGTGGCAGAGGGTCTTTGAAGTGC
TGTCACATGACAGTGATAATGAATACGCCATGTTAGACTCCACCATTGTACGTGCTCATCAACACAGCGCTGGGAAAAAAAAATCAGGCGATTGGACGCA
GTAAAGGTGGCTTAAGCACCAAAATTCATGCCCGTACTGATGCTTTAGGCAACCCAACAGGATTTTATCTCACTGATGGGCAGGCCCATGACTTAAACGG
TTCAGATGTGTTGCTGGACTTATCGTTGAGCCAGACCTGGCTTATGGATAAAGCCTACAACTCCAGGGATCGGGTCATTGCTCCAATTTTAGCGGCGAAG
GGTCAAATTGTGATGCCAGCGAAAAGTAATTCTATTGATCAGCGTGATTATGACCGACATCTCTACAAGGCAAGACATCTGATCGAGAACTTTTTTGCCA
AGCTCAAGCAGTATCGTGCTATTGCCACACGCTATGATAAATTGGCTCAAAACTTCTTATCAGCGATCTACTTAGCATCAACAATCATCTCCATTAATTG
ATGACACGCCC
AGATCTGTTACCTGGACGAATGGGTACTGTTGGAGTAACGGCCAAAGACAACCGACTATTTGTTGAAGCAGTATTGTATCGGTATCGTTCAGGTATCCCG
TGGCGAGACTTACCAGAACGCTTTGGTGATTTTAGAGTCGTGCATACTCGTTTTAGCCGTTGGGCTAAAACAGGTGTGTGGCAGAGGGTCTTTGAAGTGC
TGTCACATGACAGTGATAATGAATACGCCATGTTAGACTCCACCATTGTACGTGCTCATCAACACAGCGCTGGGAAAAAAAAATCAGGCGATTGGACGCA
GTAAAGGTGGCTTAAGCACCAAAATTCATGCCCGTACTGATGCTTTAGGCAACCCAACAGGATTTTATCTCACTGATGGGCAGGCCCATGACTTAAACGG
TTCAGATGTGTTGCTGGACTTATCGTTGAGCCAGACCTGGCTTATGGATAAAGCCTACAACTCCAGGGATCGGGTCATTGCTCCAATTTTAGCGGCGAAG
GGTCAAATTGTGATGCCAGCGAAAAGTAATTCTATTGATCAGCGTGATTATGACCGACATCTCTACAAGGCAAGACATCTGATCGAGAACTTTTTTGCCA
AGCTCAAGCAGTATCGTGCTATTGCCACACGCTATGATAAATTGGCTCAAAACTTCTTATCAGCGATCTACTTAGCATCAACAATCATCTCCATTAATTG
ATGACACGCCC
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 351 bp | 116 aa | 54 | 404 | + | No |
Description : First part of the transposase
ORF sequence :
MTKRYALRDDQWEQIKDLLPGRMGTVGVTAKDNRLFVEAVLYRYRSGIPWRDLPERFGDFRVVHTRFSRWAKTGVWQRVFEVLSHDSDNEYAMLDSTIVR
AHQHSAGKKKSGDWTQ
AHQHSAGKKKSGDWTQ
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 465 bp | 168 aa | 337 | 801 | + | No |
Description : Second part of the transposase
ORF sequence :
TPPLYVLINTALGKKNQAIGRSKGGLSTKIHARTDALGNPTGFYLTDGQAHDLNGSDVLLDLSLSQTWLMDKAYNSRDRVIAPILAAKGQIVMPAKSNSI
DQRDYDRHLYKARHLIENFFAKLKQYRAIATRYDKLAQNFLSAIYLASTIISIN
DQRDYDRHLYKARHLIENFFAKLKQYRAIATRYDKLAQNFLSAIYLASTIISIN
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 748 bp | 263 aa | 54 | 801 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKRYALRDDQWEQIKDLLPGRMGTVGVTAKDNRLFVEAVLYRYRSGIPWRDLPERFGDFRVVHTRFSRWAKTGVWQRVFEVLSHDSDNEYAMLDSTIVR
AHQHSAGKKTPPLYVLINTALGKKNQAIGRSKGGLSTKIHARTDALGNPTGFYLTDGQAHDLNGSDVLLDLSLSQTWLMDKAYNSRDRVIAPILAAKGQI
VMPAKSNSIDQRDYDRHLYKARHLIENFFAKLKQYRAIATRYDKLAQNFLSAIYLASTIISIN
AHQHSAGKKTPPLYVLINTALGKKNQAIGRSKGGLSTKIHARTDALGNPTGFYLTDGQAHDLNGSDVLLDLSLSQTWLMDKAYNSRDRVIAPILAAKGQI
VMPAKSNSIDQRDYDRHLYKARHLIENFFAKLKQYRAIATRYDKLAQNFLSAIYLASTIISIN
Blast result :Comments : The third ORF is a putative ORFAB transposase reconstructed in silico by a possible +1 frameshift.
Comments
ISAlw13 is 83% similar to ISPssp5.
References