ISAlw31
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP019144 | ND | Acinetobacter lwoffii | Acinetobacter lwoffii Acinetobacter lwoffii ZS207 plasmid pmZS |
DNA section
IS Length : 811 bp
Ends
IR Length : 17
IRL : GAGCCTGTCATCAATTAGCTTGAGAAAATCTAACCTGTCCTCATTATTTT
IRR : GGGCGTGTCATCAATTAAGCCATATCATGATTGAAGCAAGGTAGATTGCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TATTTAATAATAGCAAAC | TA | AGCATAATAGGCACAAA | 2 |
DNA sequence
GAGCCTGTCATCAATTAGCTTGAGAAAATCTAACCTGTCCTCATTATTTTTTCATGACTAAACGCTATGCATTAAGAGATGATCAGTGGGAGCAGATTAA
GGATCTGTTACCTGGACGAGCGAGTACTGTTGGAGTAACTGCCAAAGATAACAGGCTATTTGTTGAAGCAGTATTGTATCGGTATCGTTCAGGTATTCCA
TGGCGAGACTTACCCGAATGCTTTGGGGATTTTAGAGTTGTGCACACTCGCTTTAGCCGTTGGGTTAAAGCAGGCGTATGGCAGAAAGTTTTTGAAGTGC
TATCACATGAAAGTGATAATGAATATGCCATGCTAGACTCAACCATTGTGCGTGCTCATCAACACAGCGCCGGAAAAAAAAGAACAGGCAATCGGACGCA
GTAAAGGTGGTTTAAGTACCAAAATTCATGCCCGTACTGATGCTTTAGGCAACCCAACAGGATTTTATCTCACTGCCGGGCAGACTCATGACTTAAATGG
TGCTGATGTGCTGCTGGACTTATCGTTAAGTCAGACTTGGCTCATGGATAAAGCCTATAATTCTAAGGATCGGGTGATTGATCCAATCCATAAGATCAAG
GGTCAGATCGTGATGTCATCCAAAAGTAACGCGATTGATCAGCGTGATTATGACCAACATCTTTATAAAGCAAGACATCTGATCGAGAACTTCTTCGCCA
AGCTCAAGCAGTATCGAGGTATTGCTACGCGCTATGATAAATTGGCTCAAAACTTCCTGTCAGCAATCTACCTTGCTTCAATCATGATATGGCTTAATTG
ATGACACGCCC
GGATCTGTTACCTGGACGAGCGAGTACTGTTGGAGTAACTGCCAAAGATAACAGGCTATTTGTTGAAGCAGTATTGTATCGGTATCGTTCAGGTATTCCA
TGGCGAGACTTACCCGAATGCTTTGGGGATTTTAGAGTTGTGCACACTCGCTTTAGCCGTTGGGTTAAAGCAGGCGTATGGCAGAAAGTTTTTGAAGTGC
TATCACATGAAAGTGATAATGAATATGCCATGCTAGACTCAACCATTGTGCGTGCTCATCAACACAGCGCCGGAAAAAAAAGAACAGGCAATCGGACGCA
GTAAAGGTGGTTTAAGTACCAAAATTCATGCCCGTACTGATGCTTTAGGCAACCCAACAGGATTTTATCTCACTGCCGGGCAGACTCATGACTTAAATGG
TGCTGATGTGCTGCTGGACTTATCGTTAAGTCAGACTTGGCTCATGGATAAAGCCTATAATTCTAAGGATCGGGTGATTGATCCAATCCATAAGATCAAG
GGTCAGATCGTGATGTCATCCAAAAGTAACGCGATTGATCAGCGTGATTATGACCAACATCTTTATAAAGCAAGACATCTGATCGAGAACTTCTTCGCCA
AGCTCAAGCAGTATCGAGGTATTGCTACGCGCTATGATAAATTGGCTCAAAACTTCCTGTCAGCAATCTACCTTGCTTCAATCATGATATGGCTTAATTG
ATGACACGCCC
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 351 bp | 116 aa | 54 | 404 | + | No |
Chemistry : DDE
ORF sequence :
MTKRYALRDDQWEQIKDLLPGRASTVGVTAKDNRLFVEAVLYRYRSGIPWRDLPECFGDFRVVHTRFSRWVKAGVWQKVFEVLSHESDNEYAMLDSTIVR
AHQHSAGKKRTGNRTQ
AHQHSAGKKRTGNRTQ
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 465 bp | 154 aa | 337 | 801 | + | No |
Chemistry : DDE
ORF sequence :
TQPLCVLINTAPEKKEQAIGRSKGGLSTKIHARTDALGNPTGFYLTAGQTHDLNGADVLLDLSLSQTWLMDKAYNSKDRVIDPIHKIKGQIVMSSKSNAI
DQRDYDQHLYKARHLIENFFAKLKQYRGIATRYDKLAQNFLSAIYLASIMIWLN
DQRDYDQHLYKARHLIENFFAKLKQYRGIATRYDKLAQNFLSAIYLASIMIWLN
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 748 bp | 248 aa | 54 | 801 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKRYALRDDQWEQIKDLLPGRASTVGVTAKDNRLFVEAVLYRYRSGIPWRDLPECFGDFRVVHTRFSRWVKAGVWQKVFEVLSHESDNEYAMLDSTIVR
AHQHSAGKKEQAIGRSKGGLSTKIHARTDALGNPTGFYLTAGQTHDLNGADVLLDLSLSQTWLMDKAYNSKDRVIDPIHKIKGQIVMSSKSNAIDQRDYD
QHLYKARHLIENFFAKLKQYRGIATRYDKLAQNFLSAIYLASIMIWLN
AHQHSAGKKEQAIGRSKGGLSTKIHARTDALGNPTGFYLTAGQTHDLNGADVLLDLSLSQTWLMDKAYNSKDRVIDPIHKIKGQIVMSSKSNAIDQRDYD
QHLYKARHLIENFFAKLKQYRGIATRYDKLAQNFLSAIYLASIMIWLN
Blast result :
Comments
ISAlw31 is 86% aa similar to ISPssp5.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift. ISAlw31 is 87% similar to ISAlw13. Its closest relative (96% similar) occurs in Acinetobacter sp. ACNIH1 (CP026420.1).
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift. ISAlw31 is 87% similar to ISAlw13. Its closest relative (96% similar) occurs in Acinetobacter sp. ACNIH1 (CP026420.1).
References