ISAdh3
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Aeromonas dhakensis | Aeromonas dhakensis |
DNA section
IS Length : 1235 bp
Ends
IR Length : 26/30
IRL : TGAATCGCCCCCACTTTCCTAGACACCTCAAGAGCCTCAAACTGAGGTCA
IRR : TGAATCGCCCCGGCTTCTCTAGACACTTTTGAGCCGTTCGAAATATTGCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCTGAAGGTGTGAATCGCCCCCACTTTCCTAGACACCT | GTG | AAGTGTCTAGAGAAGCCGGGGCGATTCAGTGTTGATGT | 3 |
DNA sequence
TGAATCGCCCCCACTTTCCTAGACACCTCAAGAGCCTCAAACTGAGGTCATTCAGGAGGTGTTATGAGCAAGCAGCAACGTTACACGGAAGAATTCAAAC
TGGCCGCCGTCAAGCAAGTGACTGAGCGCCATTACCCAGTGGCAGAGGTCGCTAAGCGACTTGGTGTTTCCAGCCACAGTCTTTACGCCTGGATCAAACG
CTACAGCCAGCCCGAAGTGCAGCAACAGCATGACTCCAGCCAGCAAGCCGAAATTCGGCGCCTCAAGGCTGAACTGCGCCGCGTCACCGATGAGCGAGAT
ATCTTAAAAAAGGCCGCCGCGTACTTTGCCAAGCTGTCAGGTTAAGGTACGCCTTTATCCGGGAACACGAGTCCACGCATCCTGTCCGCAGACTGTGCCG
GATGATGAATGTCCACCCGAGCGGTTATTACGCTTGGCAACATCAGCCCTTATCTACCCGAGCACAGGAGGATCAACGCCTCCTGGGCCAGATCAAGCAA
TGTTGGCTAGAAAGCGGGGGCGTCTATGGCTATCGCAAGATCCGGGATGACCTGCGAGAAATGGGCGAGACCTGTGGCAAGCACCGGGTGGCGCGATTAA
TGCGACATGAAGGGTTACGTTCACAAACCGGCTATCACCGCCGTCCAGGCCATTATGGTGGCCGGCCAACGGTGGTTGCACCGAATCATTTGGAACGCCA
GTTCGATGTGACCGAGCCCAATAAGGTCTGGGTAACGGACATCACCTACATTCGCACGCATGAAGGCTGGCTCTATCTGGCTGTGGTATTGGATTTGTTC
TCACGACACGTCATTGGTTGGTCGATGGGGCAGCGGATCGACCGAGAGCTGGCCATCAGTGCGTTACTGATGGCGGTGTGGCGACGGCGACCCGAACAAG
AAGTGCTGGTTCATTCCGACCAAGGGAGCCAGTTTAGCAGCTATCACTGGCAGGATTTTTTGCGGGAAAACCAGCTCAAGGCCAGCATGAGTAGGCGCGG
CAATTGCCATGATAACGCGGTGGCGGAGAGTTTCTTCCAGCTCCTCAAACGCGAGCGGATCCGACGTAGAACCTATGCGGATCGCGAGGAAGCCCGCCAG
GATGTGTTCGATTACATTGAAATGTTTTACAACCCCAAGCGCCGGCACAGTTTCAACAATGGGCTATCGCCAGTAGAGTGTGAAAAGCAATATTTCGAAC
GGCTCAAAAGTGTCTAGAGAAGCCGGGGCGATTCA
TGGCCGCCGTCAAGCAAGTGACTGAGCGCCATTACCCAGTGGCAGAGGTCGCTAAGCGACTTGGTGTTTCCAGCCACAGTCTTTACGCCTGGATCAAACG
CTACAGCCAGCCCGAAGTGCAGCAACAGCATGACTCCAGCCAGCAAGCCGAAATTCGGCGCCTCAAGGCTGAACTGCGCCGCGTCACCGATGAGCGAGAT
ATCTTAAAAAAGGCCGCCGCGTACTTTGCCAAGCTGTCAGGTTAAGGTACGCCTTTATCCGGGAACACGAGTCCACGCATCCTGTCCGCAGACTGTGCCG
GATGATGAATGTCCACCCGAGCGGTTATTACGCTTGGCAACATCAGCCCTTATCTACCCGAGCACAGGAGGATCAACGCCTCCTGGGCCAGATCAAGCAA
TGTTGGCTAGAAAGCGGGGGCGTCTATGGCTATCGCAAGATCCGGGATGACCTGCGAGAAATGGGCGAGACCTGTGGCAAGCACCGGGTGGCGCGATTAA
TGCGACATGAAGGGTTACGTTCACAAACCGGCTATCACCGCCGTCCAGGCCATTATGGTGGCCGGCCAACGGTGGTTGCACCGAATCATTTGGAACGCCA
GTTCGATGTGACCGAGCCCAATAAGGTCTGGGTAACGGACATCACCTACATTCGCACGCATGAAGGCTGGCTCTATCTGGCTGTGGTATTGGATTTGTTC
TCACGACACGTCATTGGTTGGTCGATGGGGCAGCGGATCGACCGAGAGCTGGCCATCAGTGCGTTACTGATGGCGGTGTGGCGACGGCGACCCGAACAAG
AAGTGCTGGTTCATTCCGACCAAGGGAGCCAGTTTAGCAGCTATCACTGGCAGGATTTTTTGCGGGAAAACCAGCTCAAGGCCAGCATGAGTAGGCGCGG
CAATTGCCATGATAACGCGGTGGCGGAGAGTTTCTTCCAGCTCCTCAAACGCGAGCGGATCCGACGTAGAACCTATGCGGATCGCGAGGAAGCCCGCCAG
GATGTGTTCGATTACATTGAAATGTTTTACAACCCCAAGCGCCGGCACAGTTTCAACAATGGGCTATCGCCAGTAGAGTGTGAAAAGCAATATTTCGAAC
GGCTCAAAAGTGTCTAGAGAAGCCGGGGCGATTCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 282 bp | 92 aa | 64 | 345 | + | No |
Description : First part of the transposase
ORF sequence :
MSKQQRYTEEFKLAAVKQTERHYPVAEVAKRLGVSSHSLYAWIKRYSQPEVQQQHDSSQQAEIRRLKAELRRVTDERDILKKAAAYFAKLSG
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 924 bp | 288 aa | 294 | 1217 | + | No |
Description : Second part of the transposase
ORF sequence :
ARYLKKGRRVLCQAVRLRYAFIREHESTHPVRRLCRMMNVHPSGYYAWQHQPLSTRAQEDQRLLGQIKQCWLESGGVYGYRKIRDDLREMGETCGKHRVA
RLMRHEGLRSQTGYHRRPGHYGGRPTVVAPNHLERQFDVTEPNKVWVTDITYIRTHEGWLYLAVVLDLFSRHVIGWSMGQRIDRELAISALLMAVWRRRP
EQEVLVHSDQGSQFSSYHWQDFLRENQLKASMSRRGNCHDNAADREEARQDVFDYIEMFYNPKRRHSFNNGLSPVECEKQYFERLKSV
RLMRHEGLRSQTGYHRRPGHYGGRPTVVAPNHLERQFDVTEPNKVWVTDITYIRTHEGWLYLAVVLDLFSRHVIGWSMGQRIDRELAISALLMAVWRRRP
EQEVLVHSDQGSQFSSYHWQDFLRENQLKASMSRRGNCHDNAADREEARQDVFDYIEMFYNPKRRHSFNNGLSPVECEKQYFERLKSV
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1154 bp | 364 aa | 64 | 1217 | + | Yes |
Chemistry : DDE
ORF sequence :
MSKQQRYTEEFKLAAVKQTERHYPVAEVAKRLGVSSHSLYAWIKRYSQPEVQQQHDSSQQAEIRRLKAELRRVTDERDILKKGRRVLCQAVRLRYAFIRE
HESTHPVRRLCRMMNVHPSGYYAWQHQPLSTRAQEDQRLLGQIKQCWLESGGVYGYRKIRDDLREMGETCGKHRVARLMRHEGLRSQTGYHRRPGHYGGR
PTVVAPNHLERQFDVTEPNKVWVTDITYIRTHEGWLYLAVVLDLFSRHVIGWSMGQRIDRELAISALLMAVWRRRPEQEVLVHSDQGSQFSSYHWQDFLR
ENQLKASMSRRGNCHDNAADREEARQDVFDYIEMFYNPKRRHSFNNGLSPVECEKQYFERLKSV
HESTHPVRRLCRMMNVHPSGYYAWQHQPLSTRAQEDQRLLGQIKQCWLESGGVYGYRKIRDDLREMGETCGKHRVARLMRHEGLRSQTGYHRRPGHYGGR
PTVVAPNHLERQFDVTEPNKVWVTDITYIRTHEGWLYLAVVLDLFSRHVIGWSMGQRIDRELAISALLMAVWRRRPEQEVLVHSDQGSQFSSYHWQDFLR
ENQLKASMSRRGNCHDNAADREEARQDVFDYIEMFYNPKRRHSFNNGLSPVECEKQYFERLKSV
Blast result :
Comments
ISAdh3 is 92% (transposase) aa similar to ISAeca4. The third ORF is the putative ORFAB transposase reconstructed in silico by posible -1 frameshift.
References
1] Dongsheng Zhou (2020) Direct submission.