ISAfe1
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Acidithiobacillus ferroxidans | Acidithiobacillus ferroxidans ATCC 23270 |
DNA section
IS Length : 1424 bp
Ends
IR Length : 12
IRL : agatgtaATGGATGCCTCCCCCTACAGGACCGGCAGCGGGGTGCTTCAGA
IRR : ----tatATGGATGCCTCCATGTGTGCAAGAGGATTGCGATGTCTGACAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
AGATGTAATGGATGCCTCCCCCTACAGGACCGGCAGCGGGGTGCTTCAGAAGGGAGTCCCCGCTTGATCCGGACGGCATCAAGGTGCCAAGGTGGAAATT
TATCCACGTAAAGGACAAGGAGGACTCGTCATGCAGGTTACAACATACGGATTGGATTTGGCAAAAAGGGTCATGCAACTGCACTGGGTAAACCTGGAGA
CCGGCGAGATTCACCGCAAACAGTTGAAGCGCCGTGCACTCCTGGAGTTTTTCGCCAACCGCCAACCGGGAATCGTGGCGATGGAGGCTTGCGGTAGCGC
CCACTATTGGGGACGGGAATTGCGTAAGCTGGGGCATGAGGTCCGCTTGATTGCTGCCCAGTTTGTGCGTCCCTTCGTGAAGACCAACAAGAACGATGCG
GCCGATGCGGCAGCGATCTGGGAAGCCGTACAGCGGCCTGATATGCGCTTCGTGGCGGTCAAGAGTGAGGAACAGCAATCCGTTCTGTCGCTGCACCGTA
TGCGGGAGCAATTGATCAAGATGCGCACTATGCAGGTGAACCAGATCCGGGGCCTGCTCTACGAATTTGGTGCTGATCTGCCGCAGGGCCGCCAGCGGGG
GCTGAAAGAGGTTCCTGATGCTTTGGCCGACCTGGAAAAATCGATATCGCCCATGCTGCGGGACACGGTTCGTCAGCAATTAAAACGTCTTGAAGAGATG
GACAAGGATATTGCGGATATCGAAAAGCGTTTGACGCTGTGGAAGAAAGGCCAAGAGGCCGTGACTCGATTGATGGCTATCCCCGGCGTGGGGCTACTTA
CGGCAACTGCCATTATCGCCACCGTGGGAGATATGAAGTCCTTTCGTTCAGGACGGGAGTTTGCGGCGTTTCTGGGGCTGGTTCCCCGCCAGAGTGGGAC
CGGTGGCAAGGTCCGATTACTGGGGATCAGCAAACGGGGGGATACCTATCTCCGAACATTGCTGACCCATGGAGCCAGGGCGGTGGTGAACTTCCAGATC
AAGAATCGGAATCCCTGGATTGACAAACTGCTCAGTCGGCGACCCCATAATGTGGTGGTCGTGGCACAGTCCAACAAGATGGCGCGAACCATCTGGGCGC
TGCTGGTGAAAAACACAGAATATGTGGCCAATCACGCTAGGAATGCGGCCGCCCTATAGTAACAGTTACAGGATTGTTGACGTTCAGCAAAAGGAGTTGC
GCAGGCGGTACACGATTGATGGCAAAACAGGTCAGACCGCAGGAAAGGGAACCTGGAAGCTCTCTGTCCTAATAAGGACGCTTTAGAGATGAGGACTTTC
CTGGCGGATTTCATCAGGGCCAGCAGGCTTAATGTCCTGCATCAAAGGCCGGATATAAGACTGCAACCCGACCTCAACATGTCAGACATCGCAATCCTCT
TGCACACATGGAGGCATCCATATA
TATCCACGTAAAGGACAAGGAGGACTCGTCATGCAGGTTACAACATACGGATTGGATTTGGCAAAAAGGGTCATGCAACTGCACTGGGTAAACCTGGAGA
CCGGCGAGATTCACCGCAAACAGTTGAAGCGCCGTGCACTCCTGGAGTTTTTCGCCAACCGCCAACCGGGAATCGTGGCGATGGAGGCTTGCGGTAGCGC
CCACTATTGGGGACGGGAATTGCGTAAGCTGGGGCATGAGGTCCGCTTGATTGCTGCCCAGTTTGTGCGTCCCTTCGTGAAGACCAACAAGAACGATGCG
GCCGATGCGGCAGCGATCTGGGAAGCCGTACAGCGGCCTGATATGCGCTTCGTGGCGGTCAAGAGTGAGGAACAGCAATCCGTTCTGTCGCTGCACCGTA
TGCGGGAGCAATTGATCAAGATGCGCACTATGCAGGTGAACCAGATCCGGGGCCTGCTCTACGAATTTGGTGCTGATCTGCCGCAGGGCCGCCAGCGGGG
GCTGAAAGAGGTTCCTGATGCTTTGGCCGACCTGGAAAAATCGATATCGCCCATGCTGCGGGACACGGTTCGTCAGCAATTAAAACGTCTTGAAGAGATG
GACAAGGATATTGCGGATATCGAAAAGCGTTTGACGCTGTGGAAGAAAGGCCAAGAGGCCGTGACTCGATTGATGGCTATCCCCGGCGTGGGGCTACTTA
CGGCAACTGCCATTATCGCCACCGTGGGAGATATGAAGTCCTTTCGTTCAGGACGGGAGTTTGCGGCGTTTCTGGGGCTGGTTCCCCGCCAGAGTGGGAC
CGGTGGCAAGGTCCGATTACTGGGGATCAGCAAACGGGGGGATACCTATCTCCGAACATTGCTGACCCATGGAGCCAGGGCGGTGGTGAACTTCCAGATC
AAGAATCGGAATCCCTGGATTGACAAACTGCTCAGTCGGCGACCCCATAATGTGGTGGTCGTGGCACAGTCCAACAAGATGGCGCGAACCATCTGGGCGC
TGCTGGTGAAAAACACAGAATATGTGGCCAATCACGCTAGGAATGCGGCCGCCCTATAGTAACAGTTACAGGATTGTTGACGTTCAGCAAAAGGAGTTGC
GCAGGCGGTACACGATTGATGGCAAAACAGGTCAGACCGCAGGAAAGGGAACCTGGAAGCTCTCTGTCCTAATAAGGACGCTTTAGAGATGAGGACTTTC
CTGGCGGATTTCATCAGGGCCAGCAGGCTTAATGTCCTGCATCAAAGGCCGGATATAAGACTGCAACCCGACCTCAACATGTCAGACATCGCAATCCTCT
TGCACACATGGAGGCATCCATATA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1029 bp | 342 aa | 131 | 1159 | + | No |
Chemistry : DEDD
ORF sequence :
MQVTTYGLDLAKRVMQLHWVNLETGEIHRKQLKRRALLEFFANRQPGIVAMEACGSAHYWGRELRKLGHEVRLIAAQFVRPFVKTNKNDAADAAAIWEAV
QRPDMRFVAVKSEEQQSVLSLHRMREQLIKMRTMQVNQIRGLLYEFGADLPQGRQRGLKEVPDALADLEKSISPMLRDTVRQQLKRLEEMDKDIADIEKR
LTLWKKGQEAVTRLMAIPGVGLLTATAIIATVGDMKSFRSGREFAAFLGLVPRQSGTGGKVRLLGISKRGDTYLRTLLTHGARAVVNFQIKNRNPWIDKL
LSRRPHNVVVVAQSNKMARTIWALLVKNTEYVANHARNAAAL
QRPDMRFVAVKSEEQQSVLSLHRMREQLIKMRTMQVNQIRGLLYEFGADLPQGRQRGLKEVPDALADLEKSISPMLRDTVRQQLKRLEEMDKDIADIEKR
LTLWKKGQEAVTRLMAIPGVGLLTATAIIATVGDMKSFRSGREFAAFLGLVPRQSGTGGKVRLLGISKRGDTYLRTLLTHGARAVVNFQIKNRNPWIDKL
LSRRPHNVVVVAQSNKMARTIWALLVKNTEYVANHARNAAAL
Blast result :
Comments
No uninterrupted target sequence was found, so the ends of the IS have been defined by analogy with other IS1111 family elements, assuming that 7 nt separate IRl from the left-hand end of the element and 4 nt separate IRr from the right-hand end. The first residue of the sequence may in fact belong as the final residue, giving 6 nt on the left and 4 on the right.
There are 2 copies of ISAfe1 in the Acidithiobacillus ferroxidans ATCC 23270 genome and the transposase protein is 42% identical to that of IS1111.
There are 2 copies of ISAfe1 in the Acidithiobacillus ferroxidans ATCC 23270 genome and the transposase protein is 42% identical to that of IS1111.
References
1] The Institute for Genomic Research http://www.tigr.org
2] Partridge and Hall (2003) J. Bacteriol. 185 6371-6384
2] Partridge and Hall (2003) J. Bacteriol. 185 6371-6384