ISAzvi2
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP001157 | ND | Azotobacter vinelandii | Azotobacter vinelandii DJ |
DNA section
IS Length : 1396 bp
Ends
IR Length : 13
IRL : aatgaaATGGACTCCCCCCTCCTACGGCGTCAATGCACCAGACTGAAGTT
IRR : ---tatATGGACTCCCCCCGGTTTGCCAGTGATTTGCCCTGCCGACGAAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
AATGAAATGGACTCCCCCCTCCTACGGCGTCAATGCACCAGACTGAAGTTGCTGGAAACAGCAGTCACCAGAGGAGAGCGTCCCCATGAAACTCAAGCGG
ATAGGCGTCGATTTGGCAAAGCAGGTGTTCCAGATTCATGGCGTCGACAGCCACGAGCAGGCGGTCTGTCGCAAACAGCTCAAACGCTCGCAAATGCTGG
ACTTCTTTCGTCAACTCGAACCCTGCACGGTGGCGATGGAGGCCTGCGGCAGCTCCCATTACTGGGCGCGCGAGCTGAGAGTCCTGGGACATGAGGTGCG
ACTGATCGCACCGCAGTTCGTCAAACCCTACGTCAAGAGCGGCAAGAACGACGCCAATGACGCCGAAGCGATCTGCGAGGCGGCCAGTCGACCGAACATG
CGTTACGTCGAGGTGAAGAGCGCCGAGCAGCAGGCCGGCCAGGCCCTGCACCGGATCAGGAGCCGCCTGATCCGGGCGCGGACGGCGCTGGTCAACGAGA
TTCGCGGGTTGCTGGGCGAGTTTGGCCTGGTCGAGAGCCGGCACGGCGTGGCGGCTACCCGCCGACTGGCACAGGCGGCCTTGGCGGATGCCGAGAACGG
CCTGCCGGGCCAGATGCGCGGGTTACTGGTGGAGTTGCAAGGCGAATTGGTCGAGCATGAAGCGCGCTTGTCTCGTCTCGACGAAACGATCCAGCGCCAG
GCTCGCGAGGACGAGCGGGTGGAACGCCTGATGCTGGTCGAGGGCATCGGTCCGATCACGGCCACCGCCCTGGTGTCGGCCGTGGGCGATGCTCGCCAGT
TCCGCAGCGCCCGGCAGTTCGCTGCCTGGCTGGGGTTGGTTCCTCGCCAACACTCCAGCGGCGGCAAGGAGCAGCTGGGCCCGATCAACAAGCGAGGCGA
TACTTACTTGCGCACGCTGCTGGTTCACGGAGCCCGAGCGGTGCTCAGGCACTGCCAGGACAAGGCGGATCGACGCAGCCAGTGGCTCCAGCGCTTGCTG
TGTCGACGCAACCCGAACATCGCCACGGTCGCTTTGGCGAACAAGAATGCCCGGATCGTCTGGGCGATCCTCAGTCGGGGAGATTGCTATCAACCTGGCT
GAAAATAAAATCGCAGGCTGCACAGCCTGCAAAATGTTGAGCAGTAACCAGTAGATCCACCCCGATTGCACAGTGACATGCAGCGATGACGAACCGGTCG
AACCGGCATGTGTAAAGCCTGCACAGTGCGTCGGCCCCCTGCAAGCAAGGCCGCTAGATCAATCAGGCACACATGAGCGGATTTTCATCATGGCTCGGGG
CAGCCAATGCCTCATCGGAAGCCGGATATACGCAAGCAGTCGTACCTACGTTCGTCGGCAGGGCAAATCACTGGCAAACCGGGGGGAGTCCATATA
ATAGGCGTCGATTTGGCAAAGCAGGTGTTCCAGATTCATGGCGTCGACAGCCACGAGCAGGCGGTCTGTCGCAAACAGCTCAAACGCTCGCAAATGCTGG
ACTTCTTTCGTCAACTCGAACCCTGCACGGTGGCGATGGAGGCCTGCGGCAGCTCCCATTACTGGGCGCGCGAGCTGAGAGTCCTGGGACATGAGGTGCG
ACTGATCGCACCGCAGTTCGTCAAACCCTACGTCAAGAGCGGCAAGAACGACGCCAATGACGCCGAAGCGATCTGCGAGGCGGCCAGTCGACCGAACATG
CGTTACGTCGAGGTGAAGAGCGCCGAGCAGCAGGCCGGCCAGGCCCTGCACCGGATCAGGAGCCGCCTGATCCGGGCGCGGACGGCGCTGGTCAACGAGA
TTCGCGGGTTGCTGGGCGAGTTTGGCCTGGTCGAGAGCCGGCACGGCGTGGCGGCTACCCGCCGACTGGCACAGGCGGCCTTGGCGGATGCCGAGAACGG
CCTGCCGGGCCAGATGCGCGGGTTACTGGTGGAGTTGCAAGGCGAATTGGTCGAGCATGAAGCGCGCTTGTCTCGTCTCGACGAAACGATCCAGCGCCAG
GCTCGCGAGGACGAGCGGGTGGAACGCCTGATGCTGGTCGAGGGCATCGGTCCGATCACGGCCACCGCCCTGGTGTCGGCCGTGGGCGATGCTCGCCAGT
TCCGCAGCGCCCGGCAGTTCGCTGCCTGGCTGGGGTTGGTTCCTCGCCAACACTCCAGCGGCGGCAAGGAGCAGCTGGGCCCGATCAACAAGCGAGGCGA
TACTTACTTGCGCACGCTGCTGGTTCACGGAGCCCGAGCGGTGCTCAGGCACTGCCAGGACAAGGCGGATCGACGCAGCCAGTGGCTCCAGCGCTTGCTG
TGTCGACGCAACCCGAACATCGCCACGGTCGCTTTGGCGAACAAGAATGCCCGGATCGTCTGGGCGATCCTCAGTCGGGGAGATTGCTATCAACCTGGCT
GAAAATAAAATCGCAGGCTGCACAGCCTGCAAAATGTTGAGCAGTAACCAGTAGATCCACCCCGATTGCACAGTGACATGCAGCGATGACGAACCGGTCG
AACCGGCATGTGTAAAGCCTGCACAGTGCGTCGGCCCCCTGCAAGCAAGGCCGCTAGATCAATCAGGCACACATGAGCGGATTTTCATCATGGCTCGGGG
CAGCCAATGCCTCATCGGAAGCCGGATATACGCAAGCAGTCGTACCTACGTTCGTCGGCAGGGCAAATCACTGGCAAACCGGGGGGAGTCCATATA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1017 bp | 338 aa | 86 | 1102 | + | No |
Chemistry : DEDD
ORF sequence :
MKLKRIGVDLAKQVFQIHGVDSHEQAVCRKQLKRSQMLDFFRQLEPCTVAMEACGSSHYWARELRVLGHEVRLIAPQFVKPYVKSGKNDANDAEAICEAA
SRPNMRYVEVKSAEQQAGQALHRIRSRLIRARTALVNEIRGLLGEFGLVESRHGVAATRRLAQAALADAENGLPGQMRGLLVELQGELVEHEARLSRLDE
TIQRQAREDERVERLMLVEGIGPITATALVSAVGDARQFRSARQFAAWLGLVPRQHSSGGKEQLGPINKRGDTYLRTLLVHGARAVLRHCQDKADRRSQW
LQRLLCRRNPNIATVALANKNARIVWAILSRGDCYQPG
SRPNMRYVEVKSAEQQAGQALHRIRSRLIRARTALVNEIRGLLGEFGLVESRHGVAATRRLAQAALADAENGLPGQMRGLLVELQGELVEHEARLSRLDE
TIQRQAREDERVERLMLVEGIGPITATALVSAVGDARQFRSARQFAAWLGLVPRQHSSGGKEQLGPINKRGDTYLRTLLVHGARAVLRHCQDKADRRSQW
LQRLLCRRNPNIATVALANKNARIVWAILSRGDCYQPG
Blast result :
Comments
The IR of this IS are not at its termini. In the IS sequence as given 6 nt separate IRl from the left-hand end of the element and 3 nt separate IRr from the right-hand end. ISAzvi2 appears to be missing the nt that could be derived from either end of the element seen in most other IS1111 family members.
The transposase protein is 69% identical to that of ISPa11 and 47% identical to that of IS1111.
The transposase protein is 69% identical to that of ISPa11 and 47% identical to that of IS1111.
References
1] US Department of Energy Joint Genome Institute http://jgi.doe.gov/JGI_microbial/html/
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384