Glossary

C

• Class I Elements: (see also)
• Class II Elements: (see also)
• Cre Recombinase: The Cre Recombinase is a DNA breaking and rejoining enzyme (from tyrosine recombinase family) derived from the P1 bacteriophage. It is widely used for conditional mutagenesis of transgenes and insert DNA cassettes into eukaryotic chromosomes. (see also).
• Co-integrate:
• Copy-and-paste transposition model:
• Copy-out–Paste-in transposition model:
• Cut-and-paste transposition model:

D

• Direct Repeats (DRs):
• DDE Domain:
• Donor Primed Replicative Transposition (DPRT):

G

• Group II introns: The group II introns are mobile retroelements that use the combined activities of an autocatalytic RNA and an intron-encoded reverse transcriptase (RT) to propagate efficiently within genomes. They are intimately related with the evolution of eukaryotes, being ancestrally related to nuclear spliceosomal introns, retrotransposons and telomerase (see also).
• Genome decay:
• Genome Rearrangements:

H

• Horizontal gene transfer (or Lateral gene transfer):
• Homologous recombination:

I

• Inverted Repeats (IR):
• Integron: Integrons one class of Mobile Genetic Elements (MGEs) are specific genetic structure composed by genes (named as integron cassettes) that generally allow bacteria to adapt and rapidly evolve through the acquisition, stockpiling, excision, and reordering of open reading frames found the integron cassettes. The integron mobilization is mediated by site-specific recombination reactions by the integrase (see also).
• Invertases:

M

• Mobilome:
• Methylase (Methylation):

P

• Plasmidome:
• plasmid F:
• Pseudogenisation:
• Peel-and-paste (Single-strand) transposition model:
• Phage tyrosine integrase:
• Phage serine integrase:

R

• Resolution site:
• Resolvase:
• Rolling-circle transposition model:

S

• Synaptic complex:
• Serine recombinase: The serine recombinases are a family of DNA breaking and rejoining enzymes. Unlike homologous recombination, the serine recombinases promote rearrangements of DNA by breaking and rejoining strands at precisely defined sequence positions (see also).
• Serine Resolvases: The serine resolvases and the closely related invertases, are a group of site-specific recombinases that, in their native contexts, resolve large fused replicons into smaller separated ones
• Single-strand transposition:
• Site-specific recombination:

T

• Target specificity:
• Transposase:
• Transposome:
• Target Primed Replicative Transposition (TPRT):
• Tyrosine recombinase: Tyrosine site-specific recombinases (YRs) are a family of DNA breaking and rejoining enzymes which use the active site tyrosine nucleophile for DNA strand breakage (see also).

V

• V(D)J Recombination:

X

• Xer Site-Specific Recombination:

See also

The logic behind the TEs nomenclature and naming attribution

A committee assembled during the meeting on DNA Insertions at Cold Spring Harbor in 1976 proposed a set of rules to be used for the nomenclature of Transposable Elements . The first attempt to create an concise nomenclature system for Transposable Elements started at Stanford University by Campbell and colleagues in 1979 . They classified the prokaryotes elements as simple IS elements to more complex Tn transposons, and self-replicating episomes. In addition, definitions and nomenclature rules for these three classes of prokaryotic TEs were specified (see bellow).

TE nomenclature rules defined by Campbell (1979 and colleagues)

Classes	Definition	Naming rule
IS elements	IS elements contain no known genes unrelated to insertion functions, and are shorter than 2kb.	IS1, IS2, IS3, IS4, IS5, etc, with the numbers italicized
Tn elements	Tn are more complex, often containing two copies of an IS element. They are generally larger than 2kb, and contain additional genes unrelated to insertion functions.	Tn1, Tn2, Tn3, etc, with the numbers italicized
Episomes	Episomes are complex, self-replicating elements, often containing IS and Tn elements. Examples include the phage λ and plasmid F of E. coli.	-

The allocation of numbers and database administration was carried out by the late Dr Esther Lederberg from Stanford University Medical School, CA, USA . Lists for the registry of Tn number allocations were subsequently published .

Revised Nomenclature for Transposable Genetic Elements proposed by Roberts et al., 2008.

Type of transposable element a	Definition
Composite transposons	Flanked by IS elements. The transposase of the IS element is responsible for the catalysis of insertion and excision.
Unit transposons	Typical unit elements encode an enzyme involved in excision and integration (DD(35)E or tyrosine) often a site specific recombinase or resolvase and one or several accessory (e.g. resistance) genes in one genetic unit.
Conjugative transposons (CTns) / Integrative conjugative elements (ICEs)	The conjugative transposons (CTns), also known as integrative conjugative elements (ICEs), carry genes for excision, conjugative transfer and for integration within the new host genome. They carry a wide range of accessory genes, including antibiotic resistance
Mobilisable transposons (MTns) / Integrative mobilisable elements (IMEs)	The mobilizable transposons (MTns), also known as integrative mobilizable elements (IMEs), can be mobilized between bacterial cells by other “helper”elements that encode proteins involved in the formation of the conjugation pore or mating bridge. The MTns exploit these conjugation pores and generally provide their own DNA processing functions for intercellular transfer and subsequent transposition.
Mobile genomic islands	Some chromosomally integrated genomic islands encode tyrosine or serine site-specific recombinases that catalyze their own excision and integration but do not harbor genes involved in transfer. They carry genes encoding for a range of phenotypes. The name of a genomic island reflects the phenotype it confers, e.g. pathogenicity islands encode virulence determinants (toxins, adhesins etc).
Integrated or transposable prophage	An integrated or transposable prophage is a phage genome inserted as part of the linear structure of the chromosome of a bacterium which is able to excise and insert from and into the genome.
Integrated satellite prophage	Bacteriophage genome inserted into that of the host which requires gene products from “helper” phages to complete its replication cycle
Group I intron	Small post-transcriptionally splicing (splicing occurs in the pre-mRNA), endonuclease encoding element. Will home to allelic site
Group II intron	Small post-transcriptionally splicing (splicing occurs in the pre-mRNA), restriction endonuclease encoding element
IStron intein	Chimeric ribozyme consisting of a group I intron linked to an IS605 like transposase Small post-translational splicing (splicing occurs in the polypeptide), endonuclease encoding element. Will home to allelic site.

^a; not all reported elements have been shown to be mobile

Proposed classification system for eukaryotic Transposable Elements (TEs) (extracted from Wicker et al., 2007).