Difference between revisions of "General Information/IS and Gene Expression"

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'''<big>A</big>'''nother important aspect of IS impact on their bacterial hosts is their ability to modulate gene expression. In addition to acting as vectors for gene transmission from one replicon to another in the form composite transposons (two IS flanking any gene; [[:Image:1.2.3.png|Fig.2.3]]) and tIS [[:Image:1.13.1.png|(Fig.8.1)]] and their ability to interrupt genes, it has been known for some time<ref><nowiki><pubmed>1101028</pubmed></nowiki></ref><ref name=":0"><pubmed>6271458</pubmed></nowiki></ref> that IS can also activate gene expression. This capacity has recently received much attention due to the increase in resistance to various antibacterials<ref name=":1"><pubmed>16952941</pubmed></nowiki></ref><ref name=":2"><pubmed>12923109</pubmed></nowiki></ref><ref name=":3"><pubmed>23158541</pubmed></nowiki></ref>, a worrying public health threat<ref><nowiki><pubmed>23887414</pubmed></nowiki></ref><ref><nowiki><pubmed>23887415</pubmed></nowiki></ref>.  
 
'''<big>A</big>'''nother important aspect of IS impact on their bacterial hosts is their ability to modulate gene expression. In addition to acting as vectors for gene transmission from one replicon to another in the form composite transposons (two IS flanking any gene; [[:Image:1.2.3.png|Fig.2.3]]) and tIS [[:Image:1.13.1.png|(Fig.8.1)]] and their ability to interrupt genes, it has been known for some time<ref><nowiki><pubmed>1101028</pubmed></nowiki></ref><ref name=":0"><pubmed>6271458</pubmed></nowiki></ref> that IS can also activate gene expression. This capacity has recently received much attention due to the increase in resistance to various antibacterials<ref name=":1"><pubmed>16952941</pubmed></nowiki></ref><ref name=":2"><pubmed>12923109</pubmed></nowiki></ref><ref name=":3"><pubmed>23158541</pubmed></nowiki></ref>, a worrying public health threat<ref><nowiki><pubmed>23887414</pubmed></nowiki></ref><ref><nowiki><pubmed>23887415</pubmed></nowiki></ref>.  
  
They can accomplish this in two ways: either by providing internal promoters whose transcripts escape into neighboring DNA<ref name=":0" /><ref name=":4"><pubmed>6292860</pubmed></nowiki></ref><ref><nowiki><pubmed>6260746</pubmed></nowiki></ref><ref><nowiki><pubmed>6311437</pubmed></nowiki></ref> or by hybrid promoter formation. Many IS carry -35 promoter components oriented towards the flanking DNA [[:Image:1.24.1.png|(Fig.18.1)]]. In a number of cases this plays an important part in their transposition since a significant number of IS transpose using an excised transposon circle [[:Image:1.24.1.png|(Fig.18.1)]] with abutted left and right ends. For these IS, the other end carries a -10 element oriented inwards towards the Tpase gene. Together with the -35, this generates a strong promoter on formation of the circle junction to drive Tpase expression required for catalysis of integration [[:Image:1.24.2.png|(Fig.18.2)]] <ref><nowiki><pubmed>26350305</pubmed></nowiki></ref><ref><nowiki><pubmed>9214651</pubmed></nowiki></ref><ref><nowiki><pubmed>10438765</pubmed></nowiki></ref><ref><nowiki><pubmed>11598022</pubmed></nowiki></ref>. Thus if integration occurs next to a resident -10 sequence, the IS -35 sequence can contribute to a hybrid promoter to drive expression of neighboring genes [see <ref name=":5"><pubmed>3029382</pubmed></nowiki></ref>]. At present this phenomenon had been reported to occur with over 30 different IS in more than 17 bacterial species<ref><nowiki><pubmed>17223624</pubmed></nowiki></ref><ref><nowiki><pubmed>24499397</pubmed></nowiki></ref> ([[General Information/IS and Gene Expression#IS and Gene Expression|Table IS and Gene Expression below]]). Indeed, specific vector plasmids have been designed to identify activating insertions (e.g. <ref><nowiki><pubmed>8863735</pubmed></nowiki></ref>).
+
They can accomplish this in two ways: either by providing internal promoters whose transcripts escape into neighboring DNA<ref name=":0" /><ref name=":4"><pubmed>6292860</pubmed></nowiki></ref><ref><nowiki><pubmed>6260746</pubmed></nowiki></ref><ref><nowiki><pubmed>6311437</pubmed></nowiki></ref> or by hybrid promoter formation. Many IS carry -35 promoter components oriented towards the flanking DNA [[:Image:1.24.1.png|(Fig.18.1)]]. In a number of cases this plays an important part in their transposition since a significant number of IS transposes using an excised transposon circle [[:Image:1.24.1.png|(Fig.18.1)]] with abutted left and right ends. For these IS, the other end carries a -10 element oriented inwards towards the Tpase gene. Together with the -35, this generates a strong promoter on formation of the circle junction to drive Tpase expression required for catalysis of integration [[:Image:1.24.2.png|(Fig.18.2)]] <ref><nowiki><pubmed>26350305</pubmed></nowiki></ref><ref><nowiki><pubmed>9214651</pubmed></nowiki></ref><ref><nowiki><pubmed>10438765</pubmed></nowiki></ref><ref><nowiki><pubmed>11598022</pubmed></nowiki></ref>. Thus if integration occurs next to a resident -10 sequence, the IS -35 sequence can contribute to a hybrid promoter to drive expression of neighboring genes [see <ref name=":5"><pubmed>3029382</pubmed></nowiki></ref>]. At present this phenomenon had been reported to occur with over 30 different IS in more than 17 bacterial species<ref><nowiki><pubmed>17223624</pubmed></nowiki></ref><ref><nowiki><pubmed>24499397</pubmed></nowiki></ref> ([[General Information/IS and Gene Expression#IS and Gene Expression|Table IS and Gene Expression below]]). Indeed, specific vector plasmids have been designed to identify activating insertions (e.g. <ref><nowiki><pubmed>8863735</pubmed></nowiki></ref>).
  
 
IS activity can affect efflux mechanisms resulting in increased resistance: IS''1'' or IS''10'' insertion can up-regulate the [[wikipedia:Efflux_(microbiology)|AcrAB-TolC]] pump in ''[[wikipedia:Salmonella_enterica|Salmonella enterica]]''<ref name=":6"><pubmed>15616308</pubmed></nowiki></ref>; IS''1'' or IS''2'' insertion upstream of AcrEF<ref name=":7"><pubmed>11302812</pubmed></nowiki></ref><ref><nowiki><pubmed>11274125</pubmed></nowiki></ref> and IS''186'' insertional inactivation of the AcrAB repressor, AcrR, in ''[[wikipedia:Escherichia_coli|Escherichia coli]] <ref name=":7" />'', all lead to increased resistance to [[wikipedia:Quinolone_antibiotic|fluoroquinolones]]. Insertional inactivation of specific [[wikipedia:Porin_(protein)|porins]] can also play a significant role<ref><nowiki><pubmed>15212803</pubmed></nowiki></ref>.<center>
 
IS activity can affect efflux mechanisms resulting in increased resistance: IS''1'' or IS''10'' insertion can up-regulate the [[wikipedia:Efflux_(microbiology)|AcrAB-TolC]] pump in ''[[wikipedia:Salmonella_enterica|Salmonella enterica]]''<ref name=":6"><pubmed>15616308</pubmed></nowiki></ref>; IS''1'' or IS''2'' insertion upstream of AcrEF<ref name=":7"><pubmed>11302812</pubmed></nowiki></ref><ref><nowiki><pubmed>11274125</pubmed></nowiki></ref> and IS''186'' insertional inactivation of the AcrAB repressor, AcrR, in ''[[wikipedia:Escherichia_coli|Escherichia coli]] <ref name=":7" />'', all lead to increased resistance to [[wikipedia:Quinolone_antibiotic|fluoroquinolones]]. Insertional inactivation of specific [[wikipedia:Porin_(protein)|porins]] can also play a significant role<ref><nowiki><pubmed>15212803</pubmed></nowiki></ref>.<center>
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! scope="col" |IS family||IS name||Mechanism||Gene(s) affected||Organism||Reference||Clinical/Experimental
 
! scope="col" |IS family||IS name||Mechanism||Gene(s) affected||Organism||Reference||Clinical/Experimental
 
|-
 
|-
| rowspan="3" |[[IS Families/IS1 family|IS<i>1</i>]]|| rowspan="3" |IS<i>1</i>||Cointegrate||<i>Em<sup>R</sup></i>|| rowspan="2" |<i>Escherichia coli</i>||<ref name=":8"><pubmed>6283551</pubmed></nowiki></ref>||C?
+
| rowspan="3" |[[IS Families/IS1 family|IS<i>1</i>]]|| rowspan="3" |[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS1A IS<i>1</i>]||Cointegrate||<i>Em<sup>R</sup></i>|| rowspan="2" |<i>Escherichia coli</i>||<ref name=":8"><pubmed>6283551</pubmed></nowiki></ref>||C?
 
|-
 
|-
 
|Copy-paste circles||<i>bla<sub>TEM-1</sub></i>||<ref name=":5" />||E
 
|Copy-paste circles||<i>bla<sub>TEM-1</sub></i>||<ref name=":5" />||E
Line 25: Line 25:
 
|—||<i>acrEF</i> pump||<i>Salmonella enterica</i>||<ref name=":6" />||E
 
|—||<i>acrEF</i> pump||<i>Salmonella enterica</i>||<ref name=":6" />||E
 
|-
 
|-
| rowspan="12" |[[IS Families/IS3 family|IS<i>3</i>]]|| rowspan="6" |IS<i>2</i>|| rowspan="6" |Copy-paste circles||<i>gal</i>|| rowspan="6" |<i>Escherichia coli</i>||<ref><nowiki><pubmed>4610339</pubmed></nowiki></ref><ref><nowiki><pubmed>339095</pubmed></nowiki></ref>||E
+
| rowspan="12" |[[IS Families/IS3 family|IS<i>3</i>]]|| rowspan="6" |[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS2 IS<i>2</i>]|| rowspan="6" |Copy-paste circles||<i>gal</i>|| rowspan="6" |<i>Escherichia coli</i>||<ref><nowiki><pubmed>4610339</pubmed></nowiki></ref><ref><nowiki><pubmed>339095</pubmed></nowiki></ref>||E
 
|-
 
|-
 
|<i>gal</i>|||<ref name=":8" /><ref name=":0" />||E
 
|<i>gal</i>|||<ref name=":8" /><ref name=":0" />||E
Line 37: Line 37:
 
|<i>acrEF</i> pump||E
 
|<i>acrEF</i> pump||E
 
|-
 
|-
| rowspan="3" |IS<i>3</i>|| rowspan="3" |Copy-paste circles||<i>argE</i>|| rowspan="3" |<i>Escherichia coli</i>||<ref name=":0" />||E
+
| rowspan="3" |[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS3 IS<i>3</i>]|| rowspan="3" |Copy-paste circles||<i>argE</i>|| rowspan="3" |<i>Escherichia coli</i>||<ref name=":0" />||E
 
|-
 
|-
 
|<i>argE</i>||<ref name=":4" />||E
 
|<i>argE</i>||<ref name=":4" />||E
Line 43: Line 43:
 
|<i>citT</i>||<ref><nowiki><pubmed>22992527</pubmed></nowiki></ref>||E
 
|<i>citT</i>||<ref><nowiki><pubmed>22992527</pubmed></nowiki></ref>||E
 
|-
 
|-
|IS<i>981</i>||Copy-paste circles||<i>ldhB</i>||<i>Lactococcus lactis</i>||<ref><nowiki><pubmed>12867459</pubmed></nowiki></ref>||E
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS981 IS<i>981</i>]||Copy-paste circles||<i>ldhB</i>||<i>Lactococcus lactis</i>||<ref><nowiki><pubmed>12867459</pubmed></nowiki></ref>||E
 
|-
 
|-
|IS<i>6110</i>||Copy-paste circles||Rv2280 and PE-PGRS gene, Rv1468c||<i>Mycobacterium tuberculosis</i>||<ref><nowiki><pubmed>15130120</pubmed></nowiki></ref>||E
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS6110 IS<i>6110</i>]||Copy-paste circles||Rv2280 and PE-PGRS gene, Rv1468c||<i>Mycobacterium tuberculosis</i>||<ref><nowiki><pubmed>15130120</pubmed></nowiki></ref>||E
 
|-
 
|-
|IS<i>Kpn8</i>||Copy-paste circles||<i>blaKCP-2</i>||<i>Escherichia coli</i>, <i>Citrobacter freundii</i>, <i>Enterobacter cloacae</i>, <i>Enterobacter aerogenes</i>, and <i>Klebsiella oxytoca||<ref><nowiki><pubmed>24433026</pubmed></nowiki></ref>||C
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=ISKpn8 IS<i>Kpn8</i>]||Copy-paste circles||<i>blaKCP-2</i>||<i>Escherichia coli</i>, <i>Citrobacter freundii</i>, <i>Enterobacter cloacae</i>, <i>Enterobacter aerogenes</i>, and <i>Klebsiella oxytoca||<ref><nowiki><pubmed>24433026</pubmed></nowiki></ref>||C
 
|-
 
|-
| rowspan="9" |[[IS Families/IS4 and related families|IS<i>4</i>]]|| rowspan="3" |IS<i>10</i>||Cut-paste (hairpin)||<i>his</i>||<i>Salmonella typhimurium</i>||<ref><nowiki><pubmed>6289329</pubmed></nowiki></ref>||E
+
| rowspan="9" |[[IS Families/IS4 and related families|IS<i>4</i>]]|| rowspan="3" |[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS10 IS<i>10</i>]||Cut-paste (hairpin)||<i>his</i>||<i>Salmonella typhimurium</i>||<ref><nowiki><pubmed>6289329</pubmed></nowiki></ref>||E
 
|-
 
|-
 
|Cut-paste||—||<i>Escherichia coli</i>||<ref><nowiki><pubmed>6311437</pubmed></nowiki></ref>||E
 
|Cut-paste||—||<i>Escherichia coli</i>||<ref><nowiki><pubmed>6311437</pubmed></nowiki></ref>||E
Line 55: Line 55:
 
|Cut-paste||<i>acrEF</i> pump||<i>Salmonella enterica</i>||<ref name=":6" />||E
 
|Cut-paste||<i>acrEF</i> pump||<i>Salmonella enterica</i>||<ref name=":6" />||E
 
|-
 
|-
|IS<i>50</i>||Cut-paste (hairpin)||<i>aph3’II</i>||<i>Escherichia coli</i>||<ref><nowiki><pubmed>6260374</pubmed></nowiki></ref>||E
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS50 IS<i>50</i>]||Cut-paste (hairpin)||<i>aph3’II</i>||<i>Escherichia coli</i>||<ref><nowiki><pubmed>6260374</pubmed></nowiki></ref>||E
 
|-
 
|-
| rowspan="2" |IS<i>1999</i>|| rowspan="2" |—||<i>bla<sub>VEB-1</sub></i>||<i>Pseudomonas aeruginosa</i>||<ref name=":2" />||C
+
| rowspan="2" |[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS1999 IS<i>1999</i>]|| rowspan="2" |—||<i>bla<sub>VEB-1</sub></i>||<i>Pseudomonas aeruginosa</i>||<ref name=":2" />||C
 
|-
 
|-
 
|<i>bla<sub>VEB-1</sub></i>/<i>bla<sub>OXA-48</sub></i>||<i>Escherichia coli</i>||<ref name=":1" />||C
 
|<i>bla<sub>VEB-1</sub></i>/<i>bla<sub>OXA-48</sub></i>||<i>Escherichia coli</i>||<ref name=":1" />||C
 
|-
 
|-
|IS<i>Pa12</i>||—||<i>blaPER-1</i>||<i>Salmonella enterica</i>, <i>Pseudomonas aeruginosa</i>, <i>Providencia stuartii</i>, <i>Acinetobacter baumannii</i>||<ref name=":9"><pubmed>12936998</pubmed></nowiki></ref>||C
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=ISPa12 IS<i>Pa12</i>]||—||<i>blaPER-1</i>||<i>Salmonella enterica</i>, <i>Pseudomonas aeruginosa</i>, <i>Providencia stuartii</i>, <i>Acinetobacter baumannii</i>||<ref name=":9"><pubmed>12936998</pubmed></nowiki></ref>||C
 
|-
 
|-
| rowspan="2" |IS<i>Aba1</i>|| rowspan="2" |—||<i>bla<sub>ampC</sub></i>|| rowspan="2" |<i>Acinetobacter baumannii</i>||<ref name=":10"><pubmed>12951337</pubmed></nowiki></ref><ref><nowiki><pubmed>14742218</pubmed></nowiki></ref><ref><nowiki><pubmed>16441449</pubmed></nowiki></ref>||C
+
| rowspan="2" |[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=ISAba1 IS<i>Aba1</i>]|| rowspan="2" |—||<i>bla<sub>ampC</sub></i>|| rowspan="2" |<i>Acinetobacter baumannii</i>||<ref name=":10"><pubmed>12951337</pubmed></nowiki></ref><ref><nowiki><pubmed>14742218</pubmed></nowiki></ref><ref><nowiki><pubmed>16441449</pubmed></nowiki></ref>||C
 
|-
 
|-
 
|<i>bla<sub>OXA-51</sub></i>/<i>bla<sub>OXA-23</sub></i>||<ref><nowiki><pubmed>16630258</pubmed></nowiki></ref>||C
 
|<i>bla<sub>OXA-51</sub></i>/<i>bla<sub>OXA-23</sub></i>||<ref><nowiki><pubmed>16630258</pubmed></nowiki></ref>||C
 
|-
 
|-
 
| rowspan="6" |[[IS Families/IS5 and related IS1182 families|IS<i>5</i>]]
 
| rowspan="6" |[[IS Families/IS5 and related IS1182 families|IS<i>5</i>]]
|IS<i>5</i>|| rowspan="6" |—||<i>EmR</i>||<i>Escherichia coli</i>||<ref name=":8" />||C?
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS5 IS<i>5</i>]|| rowspan="6" |—||<i>EmR</i>||<i>Escherichia coli</i>||<ref name=":8" />||C?
 
|-
 
|-
|ISFtu2||general||<i>Francisella tularensis</i>||<ref name=":11"><pubmed>19749055</pubmed></nowiki></ref>||Natural isolate
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=ISFtu2 IS''Ftu2'']||general||<i>Francisella tularensis</i>||<ref name=":11"><pubmed>19749055</pubmed></nowiki></ref>||Natural isolate
 
|-
 
|-
|ISVa1||(iron uptake)||<i>Vibrio anguilarum</i>||<ref><nowiki><pubmed>7568465</pubmed></nowiki></ref>||Natural isolate
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=ISVa1 IS''Va1'']||(iron uptake)||<i>Vibrio anguilarum</i>||<ref><nowiki><pubmed>7568465</pubmed></nowiki></ref>||Natural isolate
 
|-
 
|-
|IS<i>1168</i> (IS<i>1186</i>)||<i>nimA</i>, <i>nimB</i>||<i>Bacteroides</i> sp.||<ref><nowiki><pubmed>8067736</pubmed></nowiki></ref>||C
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS1168 IS<i>1168</i>] ||<i>nimA</i>, <i>nimB</i>||<i>Bacteroides</i> sp.||<ref><nowiki><pubmed>8067736</pubmed></nowiki></ref>||C
 
|-
 
|-
|IS<i>1186</i>||<i>cfiA</i>||<i>Bacteroides fragilis</i>||<ref name=":12"><pubmed>8057831</pubmed></nowiki></ref><ref><nowiki><pubmed>7545155</pubmed></nowiki></ref>||C
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS1186 IS<i>1186</i>]||<i>cfiA</i>||<i>Bacteroides fragilis</i>||<ref name=":12"><pubmed>8057831</pubmed></nowiki></ref><ref><nowiki><pubmed>7545155</pubmed></nowiki></ref>||C
 
|-
 
|-
|IS<i>402</i>||<i>bla</i>||<i>Pseudomonas cepacia</i>||<ref name=":13"><pubmed>3025189</pubmed></nowiki></ref>||E
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS402 IS<i>402</i>]||<i>bla</i>||<i>Pseudomonas cepacia</i>||<ref name=":13"><pubmed>3025189</pubmed></nowiki></ref>||E
 
|-
 
|-
 
| rowspan="6" |[[IS Families/IS6 family|IS<i>6</i>]]
 
| rowspan="6" |[[IS Families/IS6 family|IS<i>6</i>]]
|IS<i>257</i>||Cointegrate||<i>dfrA</i>||<i>Staphylococcus aureus</i>||<ref><nowiki><pubmed>7840551</pubmed></nowiki></ref>|| rowspan="6" |C
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS257 IS<i>257</i>]||Cointegrate||<i>dfrA</i>||<i>Staphylococcus aureus</i>||<ref><nowiki><pubmed>7840551</pubmed></nowiki></ref>|| rowspan="6" |C
 
|-
 
|-
|IS<i>257</i>||Cointegrate||<i>tet</i>||<i>Staphylococcus aureus</i>||<ref><nowiki><pubmed>10852863</pubmed></nowiki></ref>
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS257 IS<i>257</i>]||Cointegrate||<i>tet</i>||<i>Staphylococcus aureus</i>||<ref><nowiki><pubmed>10852863</pubmed></nowiki></ref>
 
|-
 
|-
|IS<i>1008</i>||—||<i>bla<sub>OXA-58</sub></i>||<i>Acinetobacter baumannii</i>||<ref><nowiki><pubmed>18443121</pubmed></nowiki></ref>
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS1008 IS<i>1008</i>]||—||<i>bla<sub>OXA-58</sub></i>||<i>Acinetobacter baumannii</i>||<ref><nowiki><pubmed>18443121</pubmed></nowiki></ref>
 
|-
 
|-
| rowspan="2" |IS<i>26</i>|| rowspan="2" |—||<i>aphA7</i>, <i>bla<sub>S2A</sub></i>||<i>Klebsiella pneumoniae</i>||<ref><nowiki><pubmed>2160941</pubmed></nowiki></ref>
+
| rowspan="2" |[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS26 IS<i>26</i>]|| rowspan="2" |—||<i>aphA7</i>, <i>bla<sub>S2A</sub></i>||<i>Klebsiella pneumoniae</i>||<ref><nowiki><pubmed>2160941</pubmed></nowiki></ref>
 
|-
 
|-
 
|<i>bla<sub>SHV-2a</sub></i>||<i>Pseudomonas aeruginosa</i>||<ref><nowiki><pubmed>10223953</pubmed></nowiki></ref>
 
|<i>bla<sub>SHV-2a</sub></i>||<i>Pseudomonas aeruginosa</i>||<ref><nowiki><pubmed>10223953</pubmed></nowiki></ref>
 
|-
 
|-
|IS<i>140</i>||—||<i>aac(3)</i>-III and -IV||—||<ref><nowiki><pubmed>6318050</pubmed></nowiki></ref>
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS140 IS<i>140</i>]||—||<i>aac(3)</i>-III and -IV||—||<ref><nowiki><pubmed>6318050</pubmed></nowiki></ref>
 
|-
 
|-
 
| rowspan="2" |[[IS Families/IS21 family|IS<i>21</i>]]
 
| rowspan="2" |[[IS Families/IS21 family|IS<i>21</i>]]
|IS<i>Bf1</i>|| rowspan="2" |Copy-paste circle||<i>cepA</i>||<i>Bacteroides fragilis</i>||<ref><nowiki><pubmed>7517394</pubmed></nowiki></ref>|| rowspan="2" |C
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=ISBf1 IS<i>Bf1</i>]|| rowspan="2" |Copy-paste circle||<i>cepA</i>||<i>Bacteroides fragilis</i>||<ref><nowiki><pubmed>7517394</pubmed></nowiki></ref>|| rowspan="2" |C
 
|-
 
|-
|IS<i>Kpn7</i>||<i>blaKPC</i>||<i>Klebsiellea pneumonia</i>||<ref><nowiki><pubmed>18227185</pubmed></nowiki></ref>
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=ISKpn7 IS<i>Kpn7</i>]||<i>blaKPC</i>||<i>Klebsiellea pneumonia</i>||<ref><nowiki><pubmed>18227185</pubmed></nowiki></ref>
 
|-
 
|-
 
| rowspan="6" |[[IS Families/IS30 family|IS<i>30</i>]]
 
| rowspan="6" |[[IS Families/IS30 family|IS<i>30</i>]]
|IS<i>30</i>|| rowspan="6" |Copy-paste circle||<i>galK</i>||<i>Escherichia coli</i>||<ref><nowiki><pubmed>3039299</pubmed></nowiki></ref>||E
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS30 IS<i>30</i>]|| rowspan="6" |Copy-paste circle||<i>galK</i>||<i>Escherichia coli</i>||<ref><nowiki><pubmed>3039299</pubmed></nowiki></ref>||E
 
|-
 
|-
| rowspan="2" |IS<i>18</i>||<i>aac(6’)-Ij</i>|| rowspan="2" |<i>Acinetobacter</i> sp.||<ref><nowiki><pubmed>9756793</pubmed></nowiki></ref>|| rowspan="2" |C
+
| rowspan="2" |[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS18 IS<i>18</i>]||<i>aac(6’)-Ij</i>|| rowspan="2" |<i>Acinetobacter</i> sp.||<ref><nowiki><pubmed>9756793</pubmed></nowiki></ref>|| rowspan="2" |C
 
|-
 
|-
 
|<i>blaOXA257</i>||<ref><nowiki><pubmed>23014718</pubmed></nowiki></ref>
 
|<i>blaOXA257</i>||<ref><nowiki><pubmed>23014718</pubmed></nowiki></ref>
 
|-
 
|-
| rowspan="2" |IS<i>4351</i>||<i>ermF</i>|| rowspan="2" |<i>Bacteroides fragilis</i>||<ref><nowiki><pubmed>3038844</pubmed></nowiki></ref>|| rowspan="2" |C
+
| rowspan="2" |[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS4351 IS<i>4351</i>]||<i>ermF</i>|| rowspan="2" |<i>Bacteroides fragilis</i>||<ref><nowiki><pubmed>3038844</pubmed></nowiki></ref>|| rowspan="2" |C
 
|-
 
|-
 
|<i>cfiA</i>||<ref name=":3" />
 
|<i>cfiA</i>||<ref name=":3" />
 
|-
 
|-
|IS<i>1086</i>||<i>cnrCBAT</i> (Zn<sup>R</sup>)||<i>Cupriavidus metallidurans</i>|| ||E
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS1086 IS<i>1086</i>]||<i>cnrCBAT</i> (Zn<sup>R</sup>)||<i>Cupriavidus metallidurans</i>|| ||E
 
|-
 
|-
 
| rowspan="4" |[[IS Families/IS256 family|IS<i>256</i>]]
 
| rowspan="4" |[[IS Families/IS256 family|IS<i>256</i>]]
| rowspan="2" |IS<i>256</i>|| rowspan="4" |Copy-paste circles||<i>mecA</i>||<i>Staphylococcus sciuri</i>|| rowspan="2" |<ref><nowiki><pubmed>9371438</pubmed></nowiki></ref><ref><nowiki><pubmed>12511511</pubmed></nowiki></ref>|| rowspan="2" |—
+
| rowspan="2" |[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS256 IS<i>256</i>]|| rowspan="4" |Copy-paste circles||<i>mecA</i>||<i>Staphylococcus sciuri</i>|| rowspan="2" |<ref><nowiki><pubmed>9371438</pubmed></nowiki></ref><ref><nowiki><pubmed>12511511</pubmed></nowiki></ref>|| rowspan="2" |—
 
|-
 
|-
 
|<i>llm</i>||<i>Staphylococcus aureus</i>
 
|<i>llm</i>||<i>Staphylococcus aureus</i>
 
|-
 
|-
|IS<i>1490</i>||—||<i>Burkholderia cepacia</i>||<ref><nowiki><pubmed>9098071</pubmed></nowiki></ref>||—
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS1490 IS<i>1490</i>]||—||<i>Burkholderia cepacia</i>||<ref><nowiki><pubmed>9098071</pubmed></nowiki></ref>||—
 
|-
 
|-
|IS<i>406</i>||—||<i>Burkholderia cepacia</i>||<ref name=":13" />||E
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS406 IS<i>406</i>]||—||<i>Burkholderia cepacia</i>||<ref name=":13" />||E
 
|-
 
|-
| rowspan="2" |[[IS Families/IS481 family|IS<i>481</i>]]||IS<i>481</i>|| rowspan="2" |Copy-paste circles||<i>katA</i>||<i>Bordetella pertussis</i>||<ref><nowiki><pubmed>7830550</pubmed></nowiki></ref>||—
+
| rowspan="2" |[[IS Families/IS481 family|IS<i>481</i>]]||[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS481 IS<i>481</i>]|| rowspan="2" |Copy-paste circles||<i>katA</i>||<i>Bordetella pertussis</i>||<ref><nowiki><pubmed>7830550</pubmed></nowiki></ref>||—
 
|-
 
|-
|IS<i>Rme5</i>||<i>cnrCBAT</i> (Zn R)||<i>Cupriavidus metallidurans</i>||<ref><nowiki><pubmed>27047473</pubmed></nowiki></ref>||E
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=ISRme5 IS<i>Rme5</i>]||<i>cnrCBAT</i> (Zn R)||<i>Cupriavidus metallidurans</i>||<ref><nowiki><pubmed>27047473</pubmed></nowiki></ref>||E
 
|-
 
|-
|[[IS Families/IS630 family|IS<i>630</i>]]||IS<i>Ftu1</i>||Tc-like||general||<i>Francisella tularensis</i>||<ref name=":11" />||Natural isolate
+
|[[IS Families/IS630 family|IS<i>630</i>]]||[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=ISFtu1 IS<i>Ftu1</i>]||Tc-like||general||<i>Francisella tularensis</i>||<ref name=":11" />||Natural isolate
 
|-
 
|-
 
| rowspan="3" |[[IS Families/IS982 family|IS<i>982</i>]]
 
| rowspan="3" |[[IS Families/IS982 family|IS<i>982</i>]]
| rowspan="2" |IS<i>1187</i>|| rowspan="3" |—|| rowspan="2" |<i>cfiA</i>|| rowspan="2" |<i>Bacteroides fragilis</i>||<ref name=":12" />||E+C
+
| rowspan="2" |[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS1187 IS<i>1187</i>]|| rowspan="3" |—|| rowspan="2" |<i>cfiA</i>|| rowspan="2" |<i>Bacteroides fragilis</i>||<ref name=":12" />||E+C
 
|-
 
|-
 
|<ref name=":3" /><ref name=":14"><pubmed>11344163</pubmed></nowiki></ref><ref name=":15"><pubmed>12604530</pubmed></nowiki></ref>||C
 
|<ref name=":3" /><ref name=":14"><pubmed>11344163</pubmed></nowiki></ref><ref name=":15"><pubmed>12604530</pubmed></nowiki></ref>||C
 
|-
 
|-
|IS<i>982</i>||<i>citQRP</i>||<i>Lactococcus lactis</i>||<ref><nowiki><pubmed>8602160</pubmed></nowiki></ref>||—
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS982 IS<i>982</i>]||<i>citQRP</i>||<i>Lactococcus lactis</i>||<ref><nowiki><pubmed>8602160</pubmed></nowiki></ref>||—
 
|-
 
|-
 
| rowspan="12" |[[IS Families/IS1380 family|IS<i>1380</i>]]
 
| rowspan="12" |[[IS Families/IS1380 family|IS<i>1380</i>]]
|IS<i>Bf12</i>|| rowspan="12" |—|| rowspan="6" |<i>cfiA</i>|| rowspan="6" |<i>Bacteroides fragilis</i>||<ref name=":3" />|| rowspan="12" |C
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=ISBf12 IS<i>Bf12</i>]|| rowspan="12" |—|| rowspan="6" |<i>cfiA</i>|| rowspan="6" |<i>Bacteroides fragilis</i>||<ref name=":3" />|| rowspan="12" |C
 
|-
 
|-
|IS<i>612</i>||<ref name=":15" />
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS612 IS<i>612</i>]||<ref name=":15" />
 
|-
 
|-
|IS<i>613</i>||<ref name=":15" />
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS613 IS<i>613</i>]||<ref name=":15" />
 
|-
 
|-
|IS<i>614</i>||<ref name=":3" /><ref name=":15" /><ref><nowiki><pubmed>19744834</pubmed></nowiki></ref>
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS614 IS<i>614</i>]||<ref name=":3" /><ref name=":15" /><ref><nowiki><pubmed>19744834</pubmed></nowiki></ref>
 
|-
 
|-
|IS<i>1188</i>||<ref name=":14" />
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS1188 IS<i>1188</i>]||<ref name=":14" />
 
|-
 
|-
|IS<i>942</i>||<ref name=":14" />
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS942 IS<i>942</i>]||<ref name=":14" />
 
|-
 
|-
| rowspan="5" |IS<i>Ecp1</i>||<i>bla<sub>CTX-M-15</sub></i>||Enterobacteriaceae||<ref><nowiki><pubmed>11470367</pubmed></nowiki></ref>
+
| rowspan="5" |[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=ISEcp1 IS<i>Ecp1</i>]||<i>bla<sub>CTX-M-15</sub></i>||Enterobacteriaceae||<ref><nowiki><pubmed>11470367</pubmed></nowiki></ref>
 
|-
 
|-
 
|<i>bla<sub>CTX-M-17</sub></i>||<i>Klebsiella pneumoniae</i>||<ref><nowiki><pubmed>12435670</pubmed></nowiki></ref>
 
|<i>bla<sub>CTX-M-17</sub></i>||<i>Klebsiella pneumoniae</i>||<ref><nowiki><pubmed>12435670</pubmed></nowiki></ref>
Line 156: Line 156:
 
|<i>rmtC</i>||<i>Escherichia coli</i>||<ref><nowiki><pubmed>16940134</pubmed></nowiki></ref>
 
|<i>rmtC</i>||<i>Escherichia coli</i>||<ref><nowiki><pubmed>16940134</pubmed></nowiki></ref>
 
|-
 
|-
|IS<i>1187</i>||<i>cfiA</i>||<i>Bacteroides fragilis</i>||<ref name=":14" />
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS1187 IS<i>1187</i>]||<i>cfiA</i>||<i>Bacteroides fragilis</i>||<ref name=":14" />
 
|-
 
|-
 
| rowspan="2" |[[IS Families/ISL3 family|IS<i>L3</i>]]
 
| rowspan="2" |[[IS Families/ISL3 family|IS<i>L3</i>]]
|IS<i>Sg1</i>|| rowspan="2" |Copy-paste circles||<i>sspB</i> (surface antigen)||<i>Streptococcus gordonii</i>||<ref><nowiki><pubmed>9202480</pubmed></nowiki></ref>|| rowspan="2" |—
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=ISSg1 IS<i>Sg1</i>]|| rowspan="2" |Copy-paste circles||<i>sspB</i> (surface antigen)||<i>Streptococcus gordonii</i>||<ref><nowiki><pubmed>9202480</pubmed></nowiki></ref>|| rowspan="2" |—
 
|-
 
|-
|IS<i>1411</i>||<i>pheBA</i>||<i>Pseudomonas putida</i>||<ref><nowiki><pubmed>9765560</pubmed></nowiki></ref>
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS1411 IS<i>1411</i>]||<i>pheBA</i>||<i>Pseudomonas putida</i>||<ref><nowiki><pubmed>9765560</pubmed></nowiki></ref>
 
|-
 
|-
 
| rowspan="2" |[[IS Families/ISAs1 family|IS<i>As1</i>]]
 
| rowspan="2" |[[IS Families/ISAs1 family|IS<i>As1</i>]]
|IS<i>1548</i>|| rowspan="2" |—||<i>lmb</i> (lamelin binding)||<i>Streptococcus agalactiae</i>||<ref><nowiki><pubmed>20520730</pubmed></nowiki></ref>||—
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS1548 IS<i>1548</i>]|| rowspan="2" |—||<i>lmb</i> (lamelin binding)||<i>Streptococcus agalactiae</i>||<ref><nowiki><pubmed>20520730</pubmed></nowiki></ref>||—
 
|-
 
|-
 
|nd||—||<i>Acinetobacter baumannii</i>||<ref name=":10" />||C
 
|nd||—||<i>Acinetobacter baumannii</i>||<ref name=":10" />||C
 
|-
 
|-
 
| rowspan="3" |IS<i>NYC</i>
 
| rowspan="3" |IS<i>NYC</i>
|IS<i>403</i>|| rowspan="3" |—|| rowspan="3" |bla|| rowspan="3" |<i>Burkholderia cepacia</i>|| rowspan="3" |<ref name=":13" />|| rowspan="3" |E
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS403 IS<i>403</i>]|| rowspan="3" |—|| rowspan="3" |''bla''|| rowspan="3" |<i>Burkholderia cepacia</i>|| rowspan="3" |<ref name=":13" />|| rowspan="3" |E
 
|-
 
|-
|IS<i>404</i>
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS404 IS<i>404</i>]
 
|-
 
|-
|IS<i>405</i>
+
|[https://tncentral.ncc.unesp.br/ISfinder/scripts/ficheIS.php?name=IS405 IS<i>405</i>]
 
|}
 
|}
  

Revision as of 12:10, 9 August 2021

Another important aspect of IS impact on their bacterial hosts is their ability to modulate gene expression. In addition to acting as vectors for gene transmission from one replicon to another in the form composite transposons (two IS flanking any gene; Fig.2.3) and tIS (Fig.8.1) and their ability to interrupt genes, it has been known for some time[1][2] that IS can also activate gene expression. This capacity has recently received much attention due to the increase in resistance to various antibacterials[3][4][5], a worrying public health threat[6][7].

They can accomplish this in two ways: either by providing internal promoters whose transcripts escape into neighboring DNA[2][8][9][10] or by hybrid promoter formation. Many IS carry -35 promoter components oriented towards the flanking DNA (Fig.18.1). In a number of cases this plays an important part in their transposition since a significant number of IS transposes using an excised transposon circle (Fig.18.1) with abutted left and right ends. For these IS, the other end carries a -10 element oriented inwards towards the Tpase gene. Together with the -35, this generates a strong promoter on formation of the circle junction to drive Tpase expression required for catalysis of integration (Fig.18.2) [11][12][13][14]. Thus if integration occurs next to a resident -10 sequence, the IS -35 sequence can contribute to a hybrid promoter to drive expression of neighboring genes [see [15]]. At present this phenomenon had been reported to occur with over 30 different IS in more than 17 bacterial species[16][17] (Table IS and Gene Expression below). Indeed, specific vector plasmids have been designed to identify activating insertions (e.g. [18]).

IS activity can affect efflux mechanisms resulting in increased resistance: IS1 or IS10 insertion can up-regulate the AcrAB-TolC pump in Salmonella enterica[19]; IS1 or IS2 insertion upstream of AcrEF[20][21] and IS186 insertional inactivation of the AcrAB repressor, AcrR, in Escherichia coli [20], all lead to increased resistance to fluoroquinolones. Insertional inactivation of specific porins can also play a significant role[22].

Fig.18.1. Copy out paste in (DDE).Left column. Illustrates the copy out paste in the transposition mechanism. The transposon is represented as a yellow line. Flanking sequences in the donor molecule are blue. Flanking sequences in the target molecule are green. Red circles indicate 3′OH moieties generated by Tpase-catalyzed hydrolysis at the transposon end(s). Red boxes indicate target DNA flanks that are duplicated on insertion. Top to bottom: Tpase catalyzed cleavage at the 3′ transposon ends using H2O as the nucleophile. Liberated 3′OH attack the opposite end to create a bridged molecule which then undergoes replication (dotted line) to generate a circular transponso copy and regenerate the donor molecule. The circle intermediate undergoes cleavage to generate two 3'OH ends which attack the target DNA in a staggered way resulting in integration. Repair at each end then generates the direct target repeat characteristic of many transposons. Right column. Formation of hybrid promoters by copy out -paste in transposition. The left and right terminal inverted repeats of the IS, IRL ,and IRR, are shown as a square and pointed box respectively. The component –10 and –35 promoter elements of pjunc within these ends are also shown together with the weak pIRL promoter and the direction of transcription of the transposase. In a first step, single-strand cleavage and transfer from one IR to the other is catalyzed by OrfAB produced from the weak pIRL promoter. This is indicated at the top of the figure by the curved arrow. In this case, the right end (IRR) is shown attacking the left end (IRL). The resulting figure-eight form is drawn below and shows the free 3’OH group generated on the flanking donor DNA sequence. In a second step, second-strand circularization occurs by an as yet undetermined mechanism involving host functions but independently of transposon proteins [Turlan et al., 2000 ]. The resulting IRR-IRL junction carries suitably placed -35 and –10 hexamers, separated by a canonical 17 bp spacer and form a strong pjunc (bold arrow) promoter able to promote high levels of production of IS911 proteins. Integration of the circle results in disassembly of the promoter restoring low levels of expression from pIRL. Insertion upstream of a resident -10 promoter element can bring the IR-associated -35 element at the correct distance to form a promoter and activate a downstream gene.
Fig.18.2. Promoter Activities of junction fragments from different IS circles.Results of measurements of activities of 9 IS circle junctions when placed upstream of a beta-galactosidase gene.























IS and Gene Expression

Table. IS and gene expression.
IS family IS name Mechanism Gene(s) affected Organism Reference Clinical/Experimental
IS1 IS1 Cointegrate EmR Escherichia coli [23] C?
Copy-paste circles blaTEM-1 [15] E
acrEF pump Salmonella enterica [19] E
IS3 IS2 Copy-paste circles gal Escherichia coli [24][25] E
gal [23][2] E
argE [26][20] E
EmR C?
blaampC E
acrEF pump E
IS3 Copy-paste circles argE Escherichia coli [2] E
argE [8] E
citT [27] E
IS981 Copy-paste circles ldhB Lactococcus lactis [28] E
IS6110 Copy-paste circles Rv2280 and PE-PGRS gene, Rv1468c Mycobacterium tuberculosis [29] E
ISKpn8 Copy-paste circles blaKCP-2 Escherichia coli, Citrobacter freundii, Enterobacter cloacae, Enterobacter aerogenes, and Klebsiella oxytoca [30] C
IS4 IS10 Cut-paste (hairpin) his Salmonella typhimurium [31] E
Cut-paste Escherichia coli [32] E
Cut-paste acrEF pump Salmonella enterica [19] E
IS50 Cut-paste (hairpin) aph3’II Escherichia coli [33] E
IS1999 blaVEB-1 Pseudomonas aeruginosa [4] C
blaVEB-1/blaOXA-48 Escherichia coli [3] C
ISPa12 blaPER-1 Salmonella enterica, Pseudomonas aeruginosa, Providencia stuartii, Acinetobacter baumannii [34] C
ISAba1 blaampC Acinetobacter baumannii [35][36][37] C
blaOXA-51/blaOXA-23 [38] C
IS5 IS5 EmR Escherichia coli [23] C?
ISFtu2 general Francisella tularensis [39] Natural isolate
ISVa1 (iron uptake) Vibrio anguilarum [40] Natural isolate
IS1168 nimA, nimB Bacteroides sp. [41] C
IS1186 cfiA Bacteroides fragilis [42][43] C
IS402 bla Pseudomonas cepacia [44] E
IS6 IS257 Cointegrate dfrA Staphylococcus aureus [45] C
IS257 Cointegrate tet Staphylococcus aureus [46]
IS1008 blaOXA-58 Acinetobacter baumannii [47]
IS26 aphA7, blaS2A Klebsiella pneumoniae [48]
blaSHV-2a Pseudomonas aeruginosa [49]
IS140 aac(3)-III and -IV [50]
IS21 ISBf1 Copy-paste circle cepA Bacteroides fragilis [51] C
ISKpn7 blaKPC Klebsiellea pneumonia [52]
IS30 IS30 Copy-paste circle galK Escherichia coli [53] E
IS18 aac(6’)-Ij Acinetobacter sp. [54] C
blaOXA257 [55]
IS4351 ermF Bacteroides fragilis [56] C
cfiA [5]
IS1086 cnrCBAT (ZnR) Cupriavidus metallidurans E
IS256 IS256 Copy-paste circles mecA Staphylococcus sciuri [57][58]
llm Staphylococcus aureus
IS1490 Burkholderia cepacia [59]
IS406 Burkholderia cepacia [44] E
IS481 IS481 Copy-paste circles katA Bordetella pertussis [60]
ISRme5 cnrCBAT (Zn R) Cupriavidus metallidurans [61] E
IS630 ISFtu1 Tc-like general Francisella tularensis [39] Natural isolate
IS982 IS1187 cfiA Bacteroides fragilis [42] E+C
[5][62][63] C
IS982 citQRP Lactococcus lactis [64]
IS1380 ISBf12 cfiA Bacteroides fragilis [5] C
IS612 [63]
IS613 [63]
IS614 [5][63][65]
IS1188 [62]
IS942 [62]
ISEcp1 blaCTX-M-15 Enterobacteriaceae [66]
blaCTX-M-17 Klebsiella pneumoniae [67]
blaCTX-M-19 Klebsiella pneumoniae [34]
blaCTX-M Kluyvera ascorbata [68]
rmtC Escherichia coli [69]
IS1187 cfiA Bacteroides fragilis [62]
ISL3 ISSg1 Copy-paste circles sspB (surface antigen) Streptococcus gordonii [70]
IS1411 pheBA Pseudomonas putida [71]
ISAs1 IS1548 lmb (lamelin binding) Streptococcus agalactiae [72]
nd Acinetobacter baumannii [35] C
ISNYC IS403 bla Burkholderia cepacia [44] E
IS404
IS405


Bibliography

  1. <pubmed>1101028</pubmed>
  2. 2.0 2.1 2.2 2.3 Glansdorff N, Charlier D, Zafarullah M . Activation of gene expression by IS2 and IS3. - Cold Spring Harb Symp Quant Biol: 1981, 45 Pt 1;153-6 [PubMed:6271458] [DOI] </nowiki>
  3. 3.0 3.1 Aubert D, Naas T, Héritier C, Poirel L, Nordmann P . Functional characterization of IS1999, an IS4 family element involved in mobilization and expression of beta-lactam resistance genes. - J Bacteriol: 2006 Sep, 188(18);6506-14 [PubMed:16952941] [DOI] </nowiki>
  4. 4.0 4.1 Aubert D, Naas T, Nordmann P . IS1999 increases expression of the extended-spectrum beta-lactamase VEB-1 in Pseudomonas aeruginosa. - J Bacteriol: 2003 Sep, 185(17);5314-9 [PubMed:12923109] [DOI] </nowiki>
  5. 5.0 5.1 5.2 5.3 5.4 Sóki J, Eitel Z, Urbán E, Nagy E, ESCMID Study Group on Anaerobic Infections. . Molecular analysis of the carbapenem and metronidazole resistance mechanisms of Bacteroides strains reported in a Europe-wide antibiotic resistance survey. - Int J Antimicrob Agents: 2013 Feb, 41(2);122-5 [PubMed:23158541] [DOI] </nowiki>
  6. <pubmed>23887414</pubmed>
  7. <pubmed>23887415</pubmed>
  8. 8.0 8.1 Charlier D, Piette J, Glansdorff N . IS3 can function as a mobile promoter in E. coli. - Nucleic Acids Res: 1982 Oct 11, 10(19);5935-48 [PubMed:6292860] [DOI] </nowiki>
  9. <pubmed>6260746</pubmed>
  10. <pubmed>6311437</pubmed>
  11. <pubmed>26350305</pubmed>
  12. <pubmed>9214651</pubmed>
  13. <pubmed>10438765</pubmed>
  14. <pubmed>11598022</pubmed>
  15. 15.0 15.1 Prentki P, Teter B, Chandler M, Galas DJ . Functional promoters created by the insertion of transposable element IS1. - J Mol Biol: 1986 Oct 5, 191(3);383-93 [PubMed:3029382] [DOI] </nowiki>
  16. <pubmed>17223624</pubmed>
  17. <pubmed>24499397</pubmed>
  18. <pubmed>8863735</pubmed>
  19. 19.0 19.1 19.2 Olliver A, Vallé M, Chaslus-Dancla E, Cloeckaert A . Overexpression of the multidrug efflux operon acrEF by insertional activation with IS1 or IS10 elements in Salmonella enterica serovar typhimurium DT204 acrB mutants selected with fluoroquinolones. - Antimicrob Agents Chemother: 2005 Jan, 49(1);289-301 [PubMed:15616308] [DOI] </nowiki>
  20. 20.0 20.1 20.2 Jellen-Ritter AS, Kern WV . Enhanced expression of the multidrug efflux pumps AcrAB and AcrEF associated with insertion element transposition in Escherichia coli mutants Selected with a fluoroquinolone. - Antimicrob Agents Chemother: 2001 May, 45(5);1467-72 [PubMed:11302812] [DOI] </nowiki>
  21. <pubmed>11274125</pubmed>
  22. <pubmed>15212803</pubmed>
  23. 23.0 23.1 23.2 Barany F, Boeke JD, Tomasz A . Staphylococcal plasmids that replicate and express erythromycin resistance in both Streptococcus pneumoniae and Escherichia coli. - Proc Natl Acad Sci U S A: 1982 May, 79(9);2991-5 [PubMed:6283551] [DOI] </nowiki>
  24. <pubmed>4610339</pubmed>
  25. <pubmed>339095</pubmed>
  26. <pubmed>6187472</pubmed>
  27. <pubmed>22992527</pubmed>
  28. <pubmed>12867459</pubmed>
  29. <pubmed>15130120</pubmed>
  30. <pubmed>24433026</pubmed>
  31. <pubmed>6289329</pubmed>
  32. <pubmed>6311437</pubmed>
  33. <pubmed>6260374</pubmed>
  34. 34.0 34.1 Poirel L, Decousser JW, Nordmann P . Insertion sequence ISEcp1B is involved in expression and mobilization of a bla(CTX-M) beta-lactamase gene. - Antimicrob Agents Chemother: 2003 Sep, 47(9);2938-45 [PubMed:12936998] [DOI] </nowiki>
  35. 35.0 35.1 Corvec S, Caroff N, Espaze E, Giraudeau C, Drugeon H, Reynaud A . AmpC cephalosporinase hyperproduction in Acinetobacter baumannii clinical strains. - J Antimicrob Chemother: 2003 Oct, 52(4);629-35 [PubMed:12951337] [DOI] </nowiki>
  36. <pubmed>14742218</pubmed>
  37. <pubmed>16441449</pubmed>
  38. <pubmed>16630258</pubmed>
  39. 39.0 39.1 Carlson PE Jr, Horzempa J, O'Dee DM, Robinson CM, Neophytou P, Labrinidis A, Nau GJ . Global transcriptional response to spermine, a component of the intramacrophage environment, reveals regulation of Francisella gene expression through insertion sequence elements. - J Bacteriol: 2009 Nov, 191(22);6855-64 [PubMed:19749055] [DOI] </nowiki>
  40. <pubmed>7568465</pubmed>
  41. <pubmed>8067736</pubmed>
  42. 42.0 42.1 Podglajen I, Breuil J, Collatz E . Insertion of a novel DNA sequence, 1S1186, upstream of the silent carbapenemase gene cfiA, promotes expression of carbapenem resistance in clinical isolates of Bacteroides fragilis. - Mol Microbiol: 1994 Apr, 12(1);105-14 [PubMed:8057831] [DOI] </nowiki>
  43. <pubmed>7545155</pubmed>
  44. 44.0 44.1 44.2 Scordilis GE, Ree H, Lessie TG . Identification of transposable elements which activate gene expression in Pseudomonas cepacia. - J Bacteriol: 1987 Jan, 169(1);8-13 [PubMed:3025189] [DOI] </nowiki>
  45. <pubmed>7840551</pubmed>
  46. <pubmed>10852863</pubmed>
  47. <pubmed>18443121</pubmed>
  48. <pubmed>2160941</pubmed>
  49. <pubmed>10223953</pubmed>
  50. <pubmed>6318050</pubmed>
  51. <pubmed>7517394</pubmed>
  52. <pubmed>18227185</pubmed>
  53. <pubmed>3039299</pubmed>
  54. <pubmed>9756793</pubmed>
  55. <pubmed>23014718</pubmed>
  56. <pubmed>3038844</pubmed>
  57. <pubmed>9371438</pubmed>
  58. <pubmed>12511511</pubmed>
  59. <pubmed>9098071</pubmed>
  60. <pubmed>7830550</pubmed>
  61. <pubmed>27047473</pubmed>
  62. 62.0 62.1 62.2 62.3 Podglajen I, Breuil J, Rohaut A, Monsempes C, Collatz E . Multiple mobile promoter regions for the rare carbapenem resistance gene of Bacteroides fragilis. - J Bacteriol: 2001 Jun, 183(11);3531-5 [PubMed:11344163] [DOI] </nowiki>
  63. 63.0 63.1 63.2 63.3 Kato N, Yamazoe K, Han CG, Ohtsubo E . New insertion sequence elements in the upstream region of cfiA in imipenem-resistant Bacteroides fragilis strains. - Antimicrob Agents Chemother: 2003 Mar, 47(3);979-85 [PubMed:12604530] [DOI] </nowiki>
  64. <pubmed>8602160</pubmed>
  65. <pubmed>19744834</pubmed>
  66. <pubmed>11470367</pubmed>
  67. <pubmed>12435670</pubmed>
  68. <pubmed>16569841</pubmed>
  69. <pubmed>16940134</pubmed>
  70. <pubmed>9202480</pubmed>
  71. <pubmed>9765560</pubmed>
  72. <pubmed>20520730</pubmed>