Difference between revisions of "General Information/ IS derivatives of Tn3 family transposons"
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|[[Image:1.15.1.png|thumb|center|510x510px|'''Fig.10.1.''' '''Tn''3'' family characteristics.'''<br />Transposons are shown as yellow boxes with their names on the left and their lengths on the right. Terminal inverted repeats are shown as black arrowheads within the box. Transposition-related genes (transposases and resolvase recombinases are shown as purple arrows), passenger genes as red arrows, for antibiotic resistance orange/yellow arrows for heavy metal resistance, bright yellow for plant pathogenicity genes orange for toxin/antitoxin genes and blue for integron integrase genes. Resolution sites are shown in green. | |[[Image:1.15.1.png|thumb|center|510x510px|'''Fig.10.1.''' '''Tn''3'' family characteristics.'''<br />Transposons are shown as yellow boxes with their names on the left and their lengths on the right. Terminal inverted repeats are shown as black arrowheads within the box. Transposition-related genes (transposases and resolvase recombinases are shown as purple arrows), passenger genes as red arrows, for antibiotic resistance orange/yellow arrows for heavy metal resistance, bright yellow for plant pathogenicity genes orange for toxin/antitoxin genes and blue for integron integrase genes. Resolution sites are shown in green. | ||
− | Note that [https://tncentral.ncc.unesp.br/ | + | Note that [https://tncentral.ncc.unesp.br/report/te/Tn1071-M65135 Tn''1071''] carries only a transposase gene and therefore could be considered an Insertion sequence.|alt=|border]] |
|[[Image:1.15.2.png|thumb|center|510x510px|'''Fig.10.2.''' Tn''3'' family resolvase configuration. | |[[Image:1.15.2.png|thumb|center|510x510px|'''Fig.10.2.''' Tn''3'' family resolvase configuration. | ||
'''A)''' Tn''3'' has divergent transposase and resolvase (serine recombinase) genes with a res (resolution, recombination site between the divergent genes. It includes 3 sites at which resolvase binds (I, II and III). | '''A)''' Tn''3'' has divergent transposase and resolvase (serine recombinase) genes with a res (resolution, recombination site between the divergent genes. It includes 3 sites at which resolvase binds (I, II and III). | ||
− | '''B)''' [https://tncentral.ncc.unesp.br/ | + | '''B)''' [https://tncentral.ncc.unesp.br/report/te/Tn501-Z00027 Tn''501''] carries co-linear transposase and resolvase genes with the res site upstream of the resolvase. |
− | '''C)''' [https://tncentral.ncc.unesp.br/ | + | '''C)''' [https://tncentral.ncc.unesp.br/report/te/Tn4430-X07651.1 Tn''4430''] has a similar configuration as [https://tncentral.ncc.unesp.br/report/te/Tn501-Z00027 Tn''501''] but its resolvase is a tyrosine recombinase and its res site, upstream of the resolvase has a more complex structure |
− | '''D)''' [https://tncentral.ncc.unesp.br/ | + | '''D)''' [https://tncentral.ncc.unesp.br/report/te/TnXax1-AE008925 Tn''Xax1''] carries two divergent genes, ''tnpS'' (a serine recombinase) and ''tnpT'' (a helper gene). The resolution site lies between the two genes and has a complex structure (figure derived from <ref><pubmed>32234815</pubmed></ref>). |alt=]] |
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Latest revision as of 04:51, 1 April 2024
Another source of ambiguity for classification purposes occurs in the Tn3 family (see section “Tn families” and "Tn3 family") (Fig.10.1 and 10.2) [1]. Tn3 family members are quite variable. They include a number of diverse passenger genes that can represent entire operons, notably mercury resistance, or individual genes involved in antibiotic resistance, breakdown of halogenated aromatics, or virulence [e.g. [2]]. They often carry integron recombination platforms, enabling them to incorporate additional resistance genes by recruiting integron cassettes [3]. Members are quite characteristic: they have long relatively well conserved IR and a particularly long Tpase (950 to 1025 aa). They also encode a site-specific recombination (“resolution”) system necessary for completion of their transposition (Grindley 2002 - Chapter 14 from Mobile DNA II book). There are a number of different resolution systems associated with different members of this family (Fig.10.2) IS1071, (Fig.10.1) composed of Tn3-like IR and Tpase gene but lacking both the site-specific recombination system and passenger genes was identified many years ago[4][5]. This clearly accords with the definition of an IS. Several other examples have now been identified (e.g. ISVsa19, ISShfr9, ISBusp1).
Bibliography
- ↑ Nicolas E, Lambin M, Dandoy D, Galloy C, Nguyen N, Oger CA, Hallet B . The Tn3-family of Replicative Transposons. - Microbiol Spectr: 2015 Aug, 3(4); [PubMed:26350313] [DOI]
- ↑ Liebert CA, Hall RM, Summers AO . Transposon Tn21, flagship of the floating genome. - Microbiol Mol Biol Rev: 1999 Sep, 63(3);507-22 [PubMed:10477306] [DOI]
- ↑ Mazel D . Integrons: agents of bacterial evolution. - Nat Rev Microbiol: 2006 Aug, 4(8);608-20 [PubMed:16845431] [DOI]
- ↑ Nakatsu C, Ng J, Singh R, Straus N, Wyndham C . Chlorobenzoate catabolic transposon Tn5271 is a composite class I element with flanking class II insertion sequences. - Proc Natl Acad Sci U S A: 1991 Oct 1, 88(19);8312-6 [PubMed:1656436] [DOI]
- ↑ Wyndham RC, Nakatsu C, Peel M, Cashore A, Ng J, Szilagyi F . Distribution of the catabolic transposon Tn5271 in a groundwater bioremediation system. - Appl Environ Microbiol: 1994 Jan, 60(1);86-93 [PubMed:8117095] [DOI]
- ↑ Lima-Mendez G, Oliveira Alvarenga D, Ross K, Hallet B, Van Melderen L, Varani AM, Chandler M . Toxin-Antitoxin Gene Pairs Found in Tn3 Family Transposons Appear To Be an Integral Part of the Transposition Module. - mBio: 2020 Mar 31, 11(2); [PubMed:32234815] [DOI]