IS Families

Fig.1.6.1. IS distribution in the prokaryotic world.

Prokaryotic Insertion Sequences (IS)

1. IS1 family 2. IS1595 family 3. IS3 family [updated on 09/22/23] 3a. Excision: A dedicated enzyme 4. IS481 family 5. IS1202 family [New Family - online on 07/02/23] 6. IS4 and related families 6a. IS701 family 6b. ISH3 family 6c. IS1634 family 7. IS5 and related IS1182 families 8. IS6 family [updated on 07/02/23] 9. IS21 family [updated on 06/20/23] 10. IS30 family [updated on 05/21/22] 11. IS66 family 12. IS110 and IS1111 families 13. IS256 family 14. IS630 family 15. IS982 family 16. IS1380 family 17. ISAs1 family 18. ISL3 family 19. ISAzo13 family 20. IS607 family [updated on 06/06/24] 21. IS91 and related ISCR families 22. IS200/IS605 family [updated on 12/09/24]

Quick Access Table: Main Characteristics and Summary of Each IS family and Sub-Groups

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Characteristics of insertion sequence families. Abbreviations: DR, duplication repeat; IS, insertion sequence; ORF, open reading frame.
Families	Sub-Groups	Typical size-range (bp)	DR (bp)	Ends	IRs	No ORFs	Frameshift	Catalytic residues	Mechanism
IS1	—	740–1180	8–9	GGnnnTG	Y	2	ORFAB	DDE	copy-and-paste and cointegrate
	single ORF	800–1200	0–9		N	1	—
	ISMhu11	900–4600	0–10		Y	2	ORFAB
IS1595	ISPna2	1000–1150	8	GGCnnTG	Y	1	—	DDNK	copy-and-paste (?)
	ISPna2+pass	1500–2600	8	—		1+pass		—
	ISH4	1000	8	CGCTCTT		1		DDNK
	IS1016	700–745	7–9	GGGgctg				DDEK
	IS1595	900–1100	8	CcTGATT				DDNK+ER4R7
	ISSod11	1000–1100	8	nnnGcnTATC				DDHK+ER4R7
	ISNwi1	1080–1200	8	ggnnatTAT				DDEK+ER4
	ISNwi1+pass	1750–4750	8	—		1+pass		—
	ISNha5	3450–7900	8	CGGnnTT		1		DDER/K
IS3	IS150	1200–1600	3–4	TG	Y	2	ORFAB	DDE	copy-and-paste
	IS407	1100–1400	4	TG
	IS51	1000–1400	3–4	TG
	IS3	1150–1750	3–4	TGa/g
	IS2	1300–1400	5	TG
IS481	—	950–1300	4–15	TGT	Y	1	—	DDE	copy-and-paste (?)
IS1202	ISAba32	1450-1870	5-6	TGT	Y	1	—	DDE	copy-and-paste
	ISTde1	1320-1780	16-17	TAT/TGT
	IS1202	1440-1900	27-28	TGT
IS4	IS10	1200–1350	9	CT	Y	1	DDE	hairpin intermediate	cut-and-paste
	IS50	1350–1550	8–9	C				hairpin intermediate
	ISPepr1	1500–1600	7–8	-T-AA				?
	IS4	1400–1600	10–13	-AAT				?
	IS4Sa	1150–1750	8–10	CA				?
	ISH8	1400–1800	10	?
	IS231	1450–5400	10–12	CAT		1 or + *		*passenger genes
IS701	—	1400–1550	4	—	Y	1	—	DDE	—
	ISAba11	—	—
ISH3	—	1225–1500	4–5	C-GT	Y	1	—	DDE	—
IS1634	—	1500–2000	5–6	C	Y	1	—	DDE	—
IS5	IS903	950–1150	9	GG	Y	1	—	DDE	—
	ISL2	850–1200	2–3	—
	ISH1	900–1150	8	-GC
	IS5	1000–1500	4	Ga/g
	IS1031	850–1050	3	GAa/g
	IS427	800–1000	2–4	Ga/g		2	ORFAB
IS1182	—	1330–1950	0–60	—	Y	1	—	DDE	—
IS6	—	700–900	8	GG	Y	1	—	DDE	co-integrate
IS21	—	1750–2600	4–8	TG	Y	2 *	—	DDE	—
IS30	—	1000–1700	2–3	—	Y	1	—	DDE	copy-and-paste
IS66	—	2000–3000	8–9	GTAA	Y	3*	—	DDE*	—
	ISBst12	1350–1900				1		DDE
IS256	—	1200–1500	8–9	Ga/g	Y	1	—	DDE	copy-and-paste
	IS1249	1300	0–10	GG
	ISC1250	1250	0–9	GG
ISH6	—	1450	8	GGT	Y	1	—	DDE	—
ISLre2	—	1500–2000	9	—	Y	1	—	DDE	—
ISKra4	ISAzba1	1400–2900	0	—	Y	1 or + *	—	DDE	—
	ISMich2	1250–1400	8	GGG		1 or 2	ORFAB
	ISKra4	1400–3700	9	GGG		1 or + *	—
IS630	—	1000–1400	2*	—	Y	1 or 2	ORFAB	DDE	cut-and-paste
IS982	—	1000	3–9	AC	Y	1	—	DDE	—
IS1380	—	1550–2000	4–5	CC	Y	1	—	DDE	—
ISAs1	—	1200–1500	8–10	CAGGG	Y	1	—	—	—
ISL3	—	1300–2300	8	GG	Y	1	—	—	—
Tn3	—	>3000	0	GGGG	Y	>1	—	DDE	co-integrate
ISAzo13	—	1250–2200	0–4	Ga/g	Y	1	—	—	—
IS110	—	1200–1550	0	—	N	1	—	DEDD	—
	IS1111	—	—	—	Y*	—	—	—	—
IS91	—	1500–2000	0	—	N	1	—	HUH/Y2	rolling-circle
IS200/IS605	IS200	600–750	0	—	0	1*	—	HUH/Y1	peel-and-paste
	IS605	1300–2000	—	—	—	2*	—	HUH/Y1**
IS607	—	1700–2500	0	—	N	2*	—	Serine**	—
ISNCY *	IS892	1600	0–8	CTAG	Y	2	ORFAB	—	—
	ISLbi1	1400–1500	5	—	Y	1
	ISMae2	1400–2400	9	CAG	Y	1
	ISPlu15	800–1000	0	—	N	1
	ISA1214	1000–1200	8–12	—	Y	2
	ISC1217	1200	6–8	TAG	Y	1
	ISM1	1300–1600	8–9	—	Y	1
	ISDol1	1600–1900	6–7	—	Y	1	—	DDE	—

* ISNCY = Insertion Sequence Not Classified Yet